Savvas N. Savvides
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View article: ACLY inhibition promotes tumour immunity and suppresses liver cancer
ACLY inhibition promotes tumour immunity and suppresses liver cancer Open
Immunosuppressive tumour microenvironments are common in cancers such as metabolic dysfunction-associated steatohepatitis (MASH)-driven hepatocellular carcinoma (HCC) (MASH-HCC) 1–3 . Although immune cell metabolism influences effector fun…
View article: Themis and Grb2 form a constitutive structural hub in T cell receptor signalling
Themis and Grb2 form a constitutive structural hub in T cell receptor signalling Open
Positive selection of thymocytes is essential for laying the foundations of the mammalian immune system that include the T cell repertoire, self-tolerance, and prevention of autoimmunity. Themis, the archetypal member of a metazoan protein…
View article: Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries
Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries Open
Activation of Anaplastic lymphoma kinase (ALK) and leukocyte tyrosine kinase (LTK) by their cognate cytokines ALKAL2 and ALKAL1 plays important roles in development, metabolism, and cancer. Recent structural studies revealed ALK/LTK-cytoki…
View article: Structural basis of pro-inflammatory signaling via the Interleukin-36 receptor (IL-36R) mediated by IL-36γ and IL-37
Structural basis of pro-inflammatory signaling via the Interleukin-36 receptor (IL-36R) mediated by IL-36γ and IL-37 Open
Interleukin-36 receptor (IL-36R) is activated by IL-36α, IL-36β, and IL-36γ to elicit pro-inflammatory signaling and is targeted in acute skin inflammation by the approved antibody spesolimab. IL-37 was recently proposed as a new IL-36R ag…
View article: Improved protein binder design using beta-pairing targeted RFdiffusion
Improved protein binder design using beta-pairing targeted RFdiffusion Open
Despite recent advances in the computational design of protein binders, designing proteins that bind with high affinity to polar protein targets remains an outstanding problem. Here we show that RFdiffusion can be conditioned to efficientl…
View article: Development of anti-murine IL-18 binding protein antibodies to stimulate IL-18 bioactivity
Development of anti-murine IL-18 binding protein antibodies to stimulate IL-18 bioactivity Open
Interleukin (IL)-18 is an immunoregulatory cytokine that acts as a potent inducer of T helper 1 and cytotoxic responses. IL-18 activity is regulated by its decoy receptor IL-18 binding protein (IL-18BP), which forms a high-affinity complex…
View article: Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody
Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody Open
Monoclonal antibodies recognizing nonprotein antigens remain largely underrepresented in our understanding of the molecular repertoire of innate and adaptive immunity. One such antibody is Mannitou, a murine IgM that recognizes paucimannos…
View article: The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode
The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode Open
Nictaba is a (GlcNAc)n-binding, stress-inducible lectin from Nicotiana tabacum that serves as a representative for the Nictaba-related lectins, a group of proteins that play pivotal roles in plant defense mechanisms and stress response pat…
View article: Improved protein binder design using beta-pairing targeted RFdiffusion
Improved protein binder design using beta-pairing targeted RFdiffusion Open
Despite recent advances in the computational design of protein binders, designing proteins that bind with high affinity to polar protein targets remains an outstanding problem. Here we show that RFdiffusion can be conditioned to efficientl…
View article: Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with distinct stoichiometries
Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with distinct stoichiometries Open
Anaplastic lymphoma kinase (ALK) and leukocyte tyrosine kinase (LTK) are receptor tyrosine kinases activated by their cognate ligands ALKAL2 and ALKAL1 resulting in pleiotropic signalling outputs in development, metabolism and cancer. Stru…
View article: Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens
Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens Open
The interaction between bacteria and the intestinal mucus is crucial during the early pathogenesis of many enteric diseases in mammals. A critical step in this process employed by both commensal and pathogenic bacteria focuses on the break…
View article: Overcoming cryo-EM map anisotropy reveals ALK-cytokine assemblies with distinct stoichiometries
Overcoming cryo-EM map anisotropy reveals ALK-cytokine assemblies with distinct stoichiometries Open
Activation of Anaplastic lymphoma kinase (ALK) and leukocyte tyrosine kinase (LTK) by their cognate cytokines ALKAL2 and ALKAL1 play important roles in development, metabolism, and cancer. Recent structural studies revealed ALK/LTK-cytokin…
View article: A Game-Based Cognitive Intervention for Young Learners with Reading Difficulties
A Game-Based Cognitive Intervention for Young Learners with Reading Difficulties Open
The study’s primary contribution is a game-based cognitive intervention tool for young learners with reading difficulties. The second contribution is the game designer's guide tool, which provides a set of recommended design guidelines for…
View article: The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode
The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode Open
Nictaba is a (GlcNAc)n-binding, stress-inducible lectin from Nicotiana tabacum that serves as a representative for the family of Nictaba-related lectins, a group of proteins that play pivotal roles in plant defense mechanisms and stress re…
View article: <i>In silico</i>design of stable single-domain antibodies with high affinity
<i>In silico</i>design of stable single-domain antibodies with high affinity Open
Antibody-based therapeutics have become indispensable in modern medicine, but traditional methods of antibody discovery often present with limitations in developability, cross-reactivity, and ethical concerns. While deep learning and gener…
View article: Case Studies on Sustainability-Oriented Innovations and Smart Farming Technologies in the Wine Industry: A Comparative Analysis of Pilots in Cyprus and Italy
Case Studies on Sustainability-Oriented Innovations and Smart Farming Technologies in the Wine Industry: A Comparative Analysis of Pilots in Cyprus and Italy Open
Addressing the urgent sustainability challenges in the wine industry, this study explores the efficacy of sustainability-oriented innovations (SOIs) and smart farming technologies (SFTs) across wine value chains in Cyprus and Italy. Utilis…
View article: Sustainability-Oriented Innovations and Smart Farming Technologies in Wine Value Chains: Assessing their Impact on Sustainability Performance
Sustainability-Oriented Innovations and Smart Farming Technologies in Wine Value Chains: Assessing their Impact on Sustainability Performance Open
Addressing the urgent sustainability challenges in the wine industry, this study explores the efficacy of sustainability-oriented innovations (SOIs) and smart farming technologies (SFTs) across wine value chains in Cyprus and Italy. Employ…
View article: Ym1 protein crystals promote type 2 immunity
Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…
View article: Ym1 protein crystals promote type 2 immunity
Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…
View article: Reviewer #1 (Public Review): Ym1 protein crystals promote type 2 immunity
Reviewer #1 (Public Review): Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…
View article: Reviewer #2 (Public Review): Ym1 protein crystals promote type 2 immunity
Reviewer #2 (Public Review): Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…
View article: Author Response: Ym1 protein crystals promote type 2 immunity
Author Response: Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…
View article: Ym1 protein crystals promote type 2 immunity
Ym1 protein crystals promote type 2 immunity Open
Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eos…