Scott E. Boyken
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View article: 1047 Protein design and inducible expression allow context-dependent, localized IL-12 activity to enhance solid tumor T cell therapies
1047 Protein design and inducible expression allow context-dependent, localized IL-12 activity to enhance solid tumor T cell therapies Open
Background IL-12 is a pleiotropic immune-stimulatory cytokine that can modulate the tumor microenvironment to promote innate and adaptive immune responses and support cytotoxic activity of T and NK cells. However, systemic delivery of IL-1…
View article: 185 Tuning the cell-cell distance via the non-signaling extracellular spacer domain of chimeric antigen receptors is critical for optimal activity
185 Tuning the cell-cell distance via the non-signaling extracellular spacer domain of chimeric antigen receptors is critical for optimal activity Open
Background Chimeric antigen receptors (CARs) are synthetic receptors that target engineered immune cell effector functions against target cells expressing specific antigens. CAR activity can be significantly improved by optimization of mul…
View article: Computational design of mechanically coupled axle-rotor protein assemblies
Computational design of mechanically coupled axle-rotor protein assemblies Open
Natural molecular machines contain protein components that undergo motion relative to each other. Designing such mechanically constrained nanoscale protein architectures with internal degrees of freedom is an outstanding challenge for comp…
View article: Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice
Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice Open
New variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue to arise and prolong the coronavirus disease 2019 (COVID-19) pandemic. Here, we used a cell-free expression workflow to rapidly screen and optimize const…
View article: Computational design of nanoscale rotational mechanics in <i>de novo</i> protein assemblies
Computational design of nanoscale rotational mechanics in <i>de novo</i> protein assemblies Open
Natural nanomachines like the F 1 /F 0 -ATPase contain protein components that undergo rotation relative to each other. Designing such mechanically constrained nanoscale protein architectures with internal degrees of freedom is an outstand…
View article: Multivalent designed proteins protect against SARS-CoV-2 variants of concern
Multivalent designed proteins protect against SARS-CoV-2 variants of concern Open
Escape variants of SARS-CoV-2 are threatening to prolong the COVID-19 pandemic. To address this challenge, we developed multivalent protein-based minibinders as potential prophylactic and therapeutic agents. Homotrimers of single minibinde…
View article: Designed protein logic to target cells with precise combinations of surface antigens
Designed protein logic to target cells with precise combinations of surface antigens Open
Logic at the cell surface A major challenge in medical interventions is to target only diseased cells. Although there are biomarkers characteristic of certain cancers, for example, it is unlikely that a single marker can specify a particul…
View article: Conditional Recruitment to a DNA-Bound CRISPR–Cas Complex Using a Colocalization-Dependent Protein Switch
Conditional Recruitment to a DNA-Bound CRISPR–Cas Complex Using a Colocalization-Dependent Protein Switch Open
To spatially control biochemical functions at specific sites within a genome, we have engineered a synthetic switch that activates when bound to its DNA target site. The system uses two CRISPR-Cas complexes to colocalize components of a de…
View article: <i>De novo</i>design of modular and tunable allosteric biosensors
<i>De novo</i>design of modular and tunable allosteric biosensors Open
Naturally occurring allosteric protein switches have been repurposed for developing novel biosensors and reporters for cellular and clinical applications 1 , but the number of such switches is limited, and engineering them is often challen…
View article: De novo design of protein logic gates
De novo design of protein logic gates Open
Designer gates Signaling in cells can occur through protein-protein interactions. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a simi…
View article: Computational design of closely related proteins that adopt two well-defined but structurally divergent folds
Computational design of closely related proteins that adopt two well-defined but structurally divergent folds Open
The plasticity of naturally occurring protein structures, which can change shape considerably in response to changes in environmental conditions, is critical to biological function. While computational methods have been used for de novo de…
View article: De novo design of a homo-trimeric amantadine-binding protein
De novo design of a homo-trimeric amantadine-binding protein Open
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C…
View article: Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering
Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering Open
In recent years, new protein engineering methods have produced more than a dozen symmetric, self‐assembling protein cages whose structures have been validated to match their design models with near‐atomic accuracy. However, many protein ca…
View article: Author response: De novo design of a homo-trimeric amantadine-binding protein
Author response: De novo design of a homo-trimeric amantadine-binding protein Open
HClNH 2 SYMMETREL® (Amantadine Hydrochloride, USP)Tablets and Syrup Rx onlyDESCRIPTION SYMMETREL (Amantadine Hydrochloride, USP) is designated generically as amantadine hydrochloride and chemically as 1-adamantanamine hydrochloride.Amantad…
View article: Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry Open
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as …
View article: Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry Open
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as …
View article: Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry Open
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as …
View article: Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry Open
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as …
View article: Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry Open
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as …
View article: De novo design of tunable, pH-driven conformational changes
De novo design of tunable, pH-driven conformational changes Open
Designed to respond Protein design has achieved success in finding sequences that fold to very stable target structures. Protein function, however, often requires conformational dynamics. Boyken et al. describe designed proteins that under…
View article: Self-Assembling 2D Arrays with <i>de Novo</i> Protein Building Blocks
Self-Assembling 2D Arrays with <i>de Novo</i> Protein Building Blocks Open
Modular self-assembly of biomolecules in two dimensions (2D) is straightforward with DNA but has been difficult to realize with proteins, due to the lack of modular specificity similar to Watson-Crick base pairing. Here we describe a gener…