Scott H. Brewer
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View article: Modulating the pH dependent photophysical properties of green fluorescent protein
Modulating the pH dependent photophysical properties of green fluorescent protein Open
The photophysical properties of superfolder green fluorescent protein (sfGFP) were successfully modulated by the replacement of tyrosine 66 in the internal chromophore with 3-nitro- l -tyrosine.
View article: Inhibition of vibrational energy flow within an aromatic scaffold via heavy atom effect
Inhibition of vibrational energy flow within an aromatic scaffold via heavy atom effect Open
The regulation of intramolecular vibrational energy redistribution (IVR) to influence energy flow within molecular scaffolds provides a way to steer fundamental processes of chemistry, such as chemical reactivity in proteins and design of …
View article: Unraveling Complex Local Protein Environments with 4-Cyano-<scp>l</scp>-phenylalanine
Unraveling Complex Local Protein Environments with 4-Cyano-<span>l</span>-phenylalanine Open
We present a multifaceted approach to effectively probe complex local protein environments utilizing the vibrational reporter unnatural amino acid (UAA) 4-cyano-l-phenylalanine (pCNPhe) in the model system superfolder green fluorescent pro…
View article: Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein
Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein Open
The spectrophotometric properties of the green fluorescent protein (GFP) result from the post-translationally cyclized chromophore composed of three amino acids including a tyrosine at the center of the β-barrel protein. Altering the amino…
View article: Employing an Unnatural Amino Acid to Examine Local Environments in Multiple Protein Systems
Employing an Unnatural Amino Acid to Examine Local Environments in Multiple Protein Systems Open
View article: 2D-IR studies of cyanamides (NCN) as spectroscopic reporters of dynamics in biomolecules: Uncovering the origin of mysterious peaks
2D-IR studies of cyanamides (NCN) as spectroscopic reporters of dynamics in biomolecules: Uncovering the origin of mysterious peaks Open
Cyanamides (NCN) have been shown to have a larger transition dipole strength than cyano-probes. In addition, they have similar structural characteristics and vibrational lifetimes to the azido-group, suggesting their utility as infrared (I…
View article: Using Molecular Dynamics Simulations to Understand IR Spectroscopy Results in Green Fluorescent Protein
Using Molecular Dynamics Simulations to Understand IR Spectroscopy Results in Green Fluorescent Protein Open
View article: Utilizing Tyrosine Analogs to Alter Photophysical Properties of Green Fluorescent Protein
Utilizing Tyrosine Analogs to Alter Photophysical Properties of Green Fluorescent Protein Open
View article: Unraveling Complex Protein Environments in Green Fluorescent Protein using the Unnatural Amino Acid 4-cyano-L-phenylalanine
Unraveling Complex Protein Environments in Green Fluorescent Protein using the Unnatural Amino Acid 4-cyano-L-phenylalanine Open
View article: Probing Local Environments of Adenylate Kinase with Unnatural Amino Acids
Probing Local Environments of Adenylate Kinase with Unnatural Amino Acids Open
View article: Extending the vibrational lifetime of azides with heavy atoms
Extending the vibrational lifetime of azides with heavy atoms Open
The vibrational lifetime of an azide reporter can be significantly extended by isotopic labeling and a heavy atom effect.
View article: Paired Spectroscopic and Crystallographic Studies of Proteases
Paired Spectroscopic and Crystallographic Studies of Proteases Open
The active sites of subtilisin and trypsin have been studied by paired IR spectroscopic and X‐ray crystallographic studies. The active site serines of the proteases were reacted with 4‐cyanobenzenesulfonyl fluoride (CBSF), an inhibitor tha…
View article: Crystal structures of green fluorescent protein with the unnatural amino acid 4-nitro-<scp>L</scp>-phenylalanine
Crystal structures of green fluorescent protein with the unnatural amino acid 4-nitro-<span>L</span>-phenylalanine Open
The X-ray crystal structures of two superfolder green fluorescent protein (sfGFP) constructs containing a genetically incorporated spectroscopic reporter unnatural amino acid, 4-nitro-L-phenylalanine (pNO 2 F), at two unique sites in the p…
View article: Probing Local Protein Environments with a Vibrational Reporter Unnatural Amino Acid
Probing Local Protein Environments with a Vibrational Reporter Unnatural Amino Acid Open
View article: Probing Local Solvation Environments in H-NOX Proteins using Unnatural Amino Acids
Probing Local Solvation Environments in H-NOX Proteins using Unnatural Amino Acids Open
View article: Investigating the Protease Active Site Environment with Vibrational Reporters and X-Ray Crystallography
Investigating the Protease Active Site Environment with Vibrational Reporters and X-Ray Crystallography Open
View article: Probing Local Solvation Environments in H-NOX Proteins using 4-Cyano-L-Phenylalanine
Probing Local Solvation Environments in H-NOX Proteins using 4-Cyano-L-Phenylalanine Open
View article: Probing Protease Active Sites with Vibrational Reporters
Probing Protease Active Sites with Vibrational Reporters Open
View article: Exploring local solvation environments of a heme protein using the spectroscopic reporter 4-cyano-<scp>l</scp>-phenylalanine
Exploring local solvation environments of a heme protein using the spectroscopic reporter 4-cyano-<span>l</span>-phenylalanine Open
Elucidating hydration status of local environments in H-NOX using a genetically encoded vibrational reporter.
View article: Two-Dimensional Infrared Study of Vibrational Coupling between Azide and Nitrile Reporters in a RNA Nucleoside
Two-Dimensional Infrared Study of Vibrational Coupling between Azide and Nitrile Reporters in a RNA Nucleoside Open
The vibrations in the azide, N3, asymmetric stretching region and nitrile, CN, symmetric stretching region of 2'-azido-5-cyano-2'-deoxyuridine (N3CNdU) are examined by two-dimensional infrared (2D IR) spectroscopy. At earlier waiting times…
View article: Kinetic Isotope Effect Provides Insight into the Vibrational Relaxation Mechanism of Aromatic Molecules: Application to Cyano-phenylalanine
Kinetic Isotope Effect Provides Insight into the Vibrational Relaxation Mechanism of Aromatic Molecules: Application to Cyano-phenylalanine Open
Varying the reduced mass of an oscillator via isotopic substitution provides a convenient means to alter its vibrational frequency and hence has found wide applications. Herein, we show that this method can also help delineate the vibratio…
View article: Synthetic Optimization of an Effective Vibrational Reporter Unnatural Amino Acid: 4-(Azidomethyl)-L-Phenylalanine
Synthetic Optimization of an Effective Vibrational Reporter Unnatural Amino Acid: 4-(Azidomethyl)-L-Phenylalanine Open
View article: Synthesis and Evaluation of Thiocyanate and Selenocyanate Derivatives of Adenosine and Phenylalanine as Vibrational Reporters
Synthesis and Evaluation of Thiocyanate and Selenocyanate Derivatives of Adenosine and Phenylalanine as Vibrational Reporters Open
View article: Synthesis and evaluation of the sensitivity and vibrational lifetimes of thiocyanate and selenocyanate infrared reporters
Synthesis and evaluation of the sensitivity and vibrational lifetimes of thiocyanate and selenocyanate infrared reporters Open
Infrared spectroscopy demonstrates that thiocyanate and selenocyanate groups are sensitive reporters with vibrational lifetimes in the 100–300 ps range.
View article: Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study
Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study Open
The X-ray crystal structures of superfolder green fluorescent protein (sfGFP) containing the spectroscopic reporter unnatural amino acids (UAAs) 4-cyano-L-phenylalanine (pCNF) or 4-ethynyl-L-phenylalanine (pCCF) at two unique sites in the …
View article: Azidoethoxyphenylalanine as a Vibrational Reporter and Click Chemistry Partner in Proteins
Azidoethoxyphenylalanine as a Vibrational Reporter and Click Chemistry Partner in Proteins Open
An unnatural amino acid, 4‐(2‐azidoethoxy)‐ L ‐phenylalanine (AePhe, 1 ), was designed and synthesized in three steps from known compounds in 54 % overall yield. The sensitivity of the IR absorption of the azide of AePhe was established by…
View article: Development of a Vibrational Hydration Ruler
Development of a Vibrational Hydration Ruler Open
View article: Tuning the Photophysical Properties of the Green Fluorescent Protein with Unnatural Amino Acids
Tuning the Photophysical Properties of the Green Fluorescent Protein with Unnatural Amino Acids Open
View article: Probing Structural Implications of Unnatural Amino Acid Incorporation into Green Fluorescent Protein
Probing Structural Implications of Unnatural Amino Acid Incorporation into Green Fluorescent Protein Open