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Guard Cell-Enriched Phosphoproteome Reveals Phosphorylation of Endomembrane Proteins in Closed Stomata Open

Anne-Marie Pullen, Scott K. Lyons, Angie L. Mordant, Laura E. Herring, Belinda S. Akpa , et al. · 2025

Control of the stomatal aperture is multifaceted, involving a complex interplay of environmental cues and intracellular signaling pathways. It is well established that changes in ion gradients drive water movement into and out of the guard…

A Covalent PFKL Activator Suppresses Tumor Growth Open

Xiaoding Jiang, Eric M. Lynch, Congcong Lyu, Crystal N. Wilson, Lauren E. Salay , et al. · 2025

Glycolysis fuels vital cellular functions and its dysregulation is implicated in cancer, neurodegeneration, antibiotic resistance and diabetes. The glycolytic dependency of cancer, known as the Warburg effect, presents a key vulnerability …

Chemoproteomic Profiling of <i>C. albicans</i> for Characterization of Antifungal Kinase Inhibitors Open

David J. Shirley, Meganathan Nandakumar, Aurora Cabrera, Bonnie Yiu, Emily Puumala , et al. · 2025

Candida albicans is a major cause of systemic candidiasis, a severe fungal infection with a ∼40% mortality rate. Yck2, a casein kinase 1 (CK1) in C. albicans, is targeted by antifungal inhibitors YK-I-02 (YK) and MN-I-157 (MN…

The regulation of reporter transgene expression for diverse biological imaging applications Open

Alexandria S. Battison, Joseph R. Merrill, Jeremy C. Borniger, Scott K. Lyons · 2025

MAPK14/p38α shapes the molecular landscape of endometrial cancer and promotes tumorigenic characteristics Open

Sayali Joseph, Xingyuan Zhang, Gaith N. Droby, Di Wu, Victoria Bae‐Jump , et al. · 2024

The molecular underpinnings of high-grade endometrial carcinoma (HGEC) metastatic growth and survival are poorly understood. Here, we show that ascites-derived and primary tumor HGEC cell lines in 3D spheroid culture faithfully recapitulat…

Supplementary Table S10A from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

RNA-seq results. Combined tables for DESeq2 output of Ctrl, MARK2/3, YAP/TAZ double knockout in 20 cancer cell lines.

Supplementary Table S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

P value for synergy score paralog combinations. Table of P value corresponding to synergy scores calculated using GEMINI.

Supplementary Figure S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, IP–western blot analysis evaluating the interaction between 14-3-3e and YAPWT and YAP5D mutants or TAZWT and TAZ4D mutants in HEK-293T cells. Aspartate mutant reversion to wild-type S/T is indicated. Data are representative of two indep…

Supplementary Table S10B from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

RNA-seq results. Combined tables for DESeq2 output of Ctrl, MARK2/3, YAP/TAZ double knockout in 20 cancer cell lines.

Supplementary Table S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Paralog CRISPR screening data 2. Table of calculated gene level log2(fold-changes) of Paralog

Supplementary Table S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Cancer cell line model information. Table of mutations and cancer driver of cell lines used in this study.

Data from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

The Hippo signaling pathway is commonly dysregulated in human cancer, which leads to a powerful tumor dependency on the YAP/TAZ transcriptional coactivators. In this study, we used paralog cotargeting CRISPR screens to identify kinases MAR…

Supplementary Figure S10 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Western blot analysis of YAP localization following doxycycline (Dox) induced empty vector control, MKIWT or MKIMUT expression for 24h in YAPC cells. Nuclear (Nuc) and cytosolic (Cyto) fractionation are indicated. (Data shown are an ext…

Supplementary Table S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Paralog CRISPR screening data. Table for sgRNA sequences and raw sequencing counts of paralog CRISPR screens in 22 cancer cell lines.

Supplementary Table S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

FDR for synergy score paralog combinations. Table of FDR corresponding to synergy scores calculated using GEMINI.

Supplementary Table S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Synergy score paralog combinations. Table of synergy scores calculated using GEMINI.

Supplementary Table S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

sgRNA sequences. Table of all sgRNA-sequences used in this study.

Supplementary Figure S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Sanger sequencing Chromatogram of NF2 locus in MDA-MB231 cells. B, C, Rescue experiment of MARK2+3dKO in Cas9+ YAPC cells following knockout of indicated genes. Data shown are the mean ± SD of %GFP+ (normalized to day 3 after infection)…

Supplementary Table S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Phosphosite identification using mass spectrometry. Combined table of peptides identified using mass spectrometry.

Supplementary Figure S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Rescue experiment of MARK2+3dKO in Cas9+ YAPC cells following knockout of indicated genes. Data shown are the mean ± SD of %GFP+ (normalized to day 3 after infection). n=3-6. P values are calculated using a mixed effects model (consider…

Supplementary Table S11 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

YAP:TEAD4 bound YAP/TAZ dependent enhancers. Peak location file for enhancers with YAP,TEAD4 binding, and reduction of signal upon YAP/TAZ knockout.

Supplementary Figure S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A,B, CRISPR screening results from 22 cancer cell lines. A, Abundance fold-change of positive controls (dgRNAs targeting essential genes n=28 paired with control) and negative controls (dgRNAs targeting non-coding regions n=54 and nontarge…

Supplementary Figure S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Western blot analysis of Cas9+ YAPC cells. B, Representative immunofluorescence (IF) images of YAP and DAPI following MARK2+3dKO in A549 cancer cells. scale bar = 30 µm. C, Comparison of mean immunofluorescence signal of YAP across the …

Supplementary Data MS from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

Mass spectrometric identification and quantification of MARK2 substrate peptides This document contains mass spectrometric data supporting phosphorylation of specific phosphorylation sites on CDC25C, YAP, TAZ, and NF2. For each considered …

Supplementary Figure S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Mutational two-class comparison between 19 MARK2/3-dependent and 12 MARK2/3-independent human cancer cell lines (shown in B). Mutation data were obtained from the CCLE database. The effect size and P values were calculated for every mut…

Supplementary Figure S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A, Growth kinetics of subcutaneous YAPC xenografts implanted in immunodeficient mice. Indicated genes were knocked out just before injection. Data are shown as mean ± SEM n=5 per group. P values are calculated using a mixed effects model (…

Supplementary Table S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

YAP/TAZ signature. Table of genes used as YAP/TAZ signature.

Supplementary Figure S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A-E, Western blot analysis of MARK2 specific substrates phosphorylation. Labeling as described in Fig 3D. Data are representative of two independent experiments. F, Lolli-pop illustration of MARK2-dependent phosphorylation sites on MST1/2 …

Supplementary Figure S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open

Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy , et al. · 2024

A-K, Fragmentation spectrum supporting peptide identity and phosphosites on YAP, TAZ, and NF2. Precursor ion chromatogram (XIC) and corresponding ions are provided in supplementary materials.

Aldehydes alter TGF-β signaling and induce obesity and cancer Open

Xiaochun Yang, Krishanu Bhowmick, Shuyun Rao, Xiyan Xiang, Kazufumi Ohshiro , et al. · 2024

Obesity and fatty liver diseases-metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH)-affect over one-third of the global population and are exacerbated in individuals…

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