Scott T. Magness
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View article: Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity
Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity Open
Summary The Planar Cell Polarity (PCP) complex regulates many diverse phenotypes. While recent literature has elucidated key mechanisms underlying PCP, a mechanistic understanding of how these components function as a system to drive polar…
View article: Replicating Host–Microbiome Interactions: Harnessing Organ-on-a-Chip and Organoid Technologies to Model Vaginal and Lung Physiology
Replicating Host–Microbiome Interactions: Harnessing Organ-on-a-Chip and Organoid Technologies to Model Vaginal and Lung Physiology Open
Organ-on-a-chip (OOC) and organoid technologies are at the forefront of developing sophisticated in vitro systems that replicate complex host–microbiome interactions, including those associated with vaginal health and lung infection. We ex…
View article: Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine
Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine Open
These studies validate an approach to identify and predict upstream regulation of restituting epithelium in a unique pig intestinal ischemic injury model. Identification of potential molecular mediators of restitution, such as CSF-1, will …
View article: Pioneering a New Frontier: Modeling the Epithelial-immune Cell Axis Using Human Intestinal Organoids
Pioneering a New Frontier: Modeling the Epithelial-immune Cell Axis Using Human Intestinal Organoids Open
View article: Sex Differences in Colonic Inflammation are Driven by Epithelial-Specific Expression of Estrogen Receptor Alpha
Sex Differences in Colonic Inflammation are Driven by Epithelial-Specific Expression of Estrogen Receptor Alpha Open
ERα signaling within IECs drives opposing sex-dependent effects on the development, regenerative capacity, and inflammatory susceptibility of the intestinal epithelium.
View article: A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics
A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics Open
BACKGROUND & AIMS: Single-cell transcriptomics offer unprecedented resolution of tissue function at the cellular level, yet studies analyzing healthy adult human small intestine and colon are sparse. Here, we present single-cell transcript…
View article: An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal epithelial cells
An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal epithelial cells Open
The intestinal epithelium dynamically controls cell cycle, yet no experimental platform exists for directly analyzing cell cycle phases in non-immortalized human intestinal epithelial cells (IECs). Here, we present two reporters and a comp…
View article: Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine
Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine Open
Intestinal ischemic injury damages the epithelial barrier predisposes patients to life-threatening sepsis unless that barrier is rapidly restored. There is an age-dependency of intestinal recovery in that neonates are the most susceptible …
View article: Comparative Approaches for Quantification of Product Yield in the Production of Recombinant Green Fluorescent Protein (GFPuv) Expressed in <i>E. coli</i>
Comparative Approaches for Quantification of Product Yield in the Production of Recombinant Green Fluorescent Protein (GFPuv) Expressed in <i>E. coli</i> Open
Process Analytical Technologies (PAT) are critical for efficient and automated bioprocessing; moreover, sensor and assay-driven automation will be necessary for the realization of Industry 4.0. Herein, we demonstrate methods for analyzing …
View article: Preparing Chamber Slides With Pressed Collagen for Live Imaging Monolayers of Primary Human Intestinal Stem Cells
Preparing Chamber Slides With Pressed Collagen for Live Imaging Monolayers of Primary Human Intestinal Stem Cells Open
Primary human intestinal stem cells (ISCs) can be cultured and passaged indefinitely as two-dimensional monolayers grown on soft collagen. Culturing ISCs as monolayers enables easy access to the luminal side for chemical treatments and pro…
View article: Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period
Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period Open
This study refines a powerful translational pig model, defining an age-dependent relationship between enteric glia and the intestinal epithelium during intestinal ischemic injury and confirming an important role for enteric glial cell (EGC…
View article: TGFB1 induces fetal reprogramming and enhances intestinal regeneration
TGFB1 induces fetal reprogramming and enhances intestinal regeneration Open
View article: An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal stem cells
An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal stem cells Open
Background and Aims The intestinal epithelium exhibits dynamic control of cell cycle phase lengths, yet no experimental platform exists for directly analyzing cell cycle phases in living human intestinal stem cells (ISCs). Here, we develop…
View article: Design of 8-mer peptides that block Clostridioides difficile toxin A in intestinal cells
Design of 8-mer peptides that block Clostridioides difficile toxin A in intestinal cells Open
View article: P30-014-23 Immunomodulatory Effects of Galactooligosaccharides
P30-014-23 Immunomodulatory Effects of Galactooligosaccharides Open
View article: A Roadmap for the Human Gut Cell Atlas
A Roadmap for the Human Gut Cell Atlas Open
View article: A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early <i>Clostridioides difficile</i> toxin exposure
A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early <i>Clostridioides difficile</i> toxin exposure Open
Novel human colonocyte monolayer cultures, benchmarked by transcriptomics for physiological relevance, detect early cytopathic impacts of Clostridioides difficile toxins TcdA and TcdB. A fluorescent ZO-1 reporter in primary human colonocyt…
View article: A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity
A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity Open
Background & Aims Hypoxia in the intestinal epithelium can be caused by acute ischemic events or conditions like Inflammatory Bowel Disease (IBD) where immune cell infiltration produces ‘inflammatory hypoxia’, a chronic condition that star…
View article: Design of 8-mer Peptides that Block<i>Clostridioides difficile</i>Toxin A in Intestinal Cells
Design of 8-mer Peptides that Block<i>Clostridioides difficile</i>Toxin A in Intestinal Cells Open
Clostridioides difficile ( C. diff .) is a bacterium that causes severe diarrhea and inflammation of the colon. The pathogenicity of C. diff . infection is derived from two major toxins, toxins A (TcdA) and B (TcdB). Peptide inhibitors tha…
View article: Hypoxia Primes Human ISCs for Interleukin-Dependent Rescue of Stem Cell Activity
Hypoxia Primes Human ISCs for Interleukin-Dependent Rescue of Stem Cell Activity Open
Hypoxia pushes hISCs into a dormant but reversible proliferative state and primes hISCs to respond to a subset of ILs that preserves hISC activity. These findings have important implications for understanding intestinal epithelial regenera…
View article: Supplemental Table S2
Supplemental Table S2 Open
Article Title: A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early Clostridioides difficile Toxin Exposure Journal: AJP-GI Supplemental Table S2. Bulk R…
View article: Supplemental Table S1
Supplemental Table S1 Open
Article Title: A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early Clostridioides difficile Toxin Exposure Journal: AJP-GI Supplemental Table S1. Bulk R…
View article: SOX9 elongates cell cycle phases and biases fate decisions in human intestinal stem cells
SOX9 elongates cell cycle phases and biases fate decisions in human intestinal stem cells Open
Background and Aims The transcription factor SOX9 is expressed in many stem/progenitor cell populations and has biphasic correlations with proliferation rates across different biological systems. In murine intestinal crypts, distinct Sox9 …
View article: A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early<i>Clostridioides difficile</i>Toxin Exposure
A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early<i>Clostridioides difficile</i>Toxin Exposure Open
Background & Aims Clostridioides difficile ( C. difficile ) toxins A (TcdA) and B (TcdB) cause antibiotic-associated colitis and increase morbidity and mortality. Accurate in vitro models are necessary to detect early toxicity kinetics, in…
View article: Dysplastic Stem Cell Plasticity Functions as a Driving Force for Neoplastic Transformation of Precancerous Gastric Mucosa
Dysplastic Stem Cell Plasticity Functions as a Driving Force for Neoplastic Transformation of Precancerous Gastric Mucosa Open
View article: Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells
Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells Open
View article: Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection
Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection Open
Significance A potential outbreak of swine acute diarrhea syndrome coronavirus (SADS-CoV) in the human population could be devastating. Using genomewide CRISPR knockout screening, we identified the placenta-associated 8 protein (PLAC8) as …
View article: Transcriptomics informs design of a planar human enterocyte culture system that reveals metformin enhances fatty acid export
Transcriptomics informs design of a planar human enterocyte culture system that reveals metformin enhances fatty acid export Open
Background & Aims Absorption, metabolism, and export of dietary lipids occurs in the small intestinal epithelium. Caco-2 and organoids have been used to study these processes but are limited in physiological relevance or preclude simultane…
View article: A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and Export
A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and Export Open
Primary human ISCs in culture undergo programmed maturation. Absorptive enterocyte monolayers show in vivo maturational states and lipid-handling gene expression profiles. Absorptive enterocytes create strong epithelial barriers in 96-Tran…
View article: Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection
Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection Open
Zoonotic transmission of coronaviruses poses an ongoing threat to human populations. Endemic outbreaks of swine acute diarrhea syndrome coronavirus (SADS-CoV) have caused severe economic losses in the pig industry and have the potential to…