Sebastian Behrens
YOU?
Author Swipe
View article: Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities
Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities Open
Quantitative real-time PCR of phylogenetic and functional marker genes is among the most commonly used techniques to quantify the abundance of microbial taxa in environmental samples. However, in most environmental applications, the approa…
View article: Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets
Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets Open
In vitro compartmentalization (IVC) is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)–phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinat…
View article: Ammonia-Oxidizing Bacteria Maintain Abundance but Lower<i>amoA</i>-Gene Expression during Cold Temperature Nitrification Failure in a Full-Scale Municipal Wastewater Treatment Plant
Ammonia-Oxidizing Bacteria Maintain Abundance but Lower<i>amoA</i>-Gene Expression during Cold Temperature Nitrification Failure in a Full-Scale Municipal Wastewater Treatment Plant Open
The diverse microbial community of activated sludge used in biological treatment systems exhibits dynamic seasonal shifts in community composition and activity. Many wastewater treatment plants in temperate/continental climates experience …
View article: Microbial Community Composition in Municipal Wastewater Treatment Bioreactors Follows a Distance Decay Pattern Primarily Controlled by Environmental Heterogeneity
Microbial Community Composition in Municipal Wastewater Treatment Bioreactors Follows a Distance Decay Pattern Primarily Controlled by Environmental Heterogeneity Open
Understanding the spatiotemporal patterns of biodiversity is a central goal of ecology. The distance decay of community similarity is one of the spatial scaling patterns observed in many forms of life, including plants, animals, and microb…
View article: Direct Evidence for Deterministic Assembly of Bacterial Communities in Full-Scale Municipal Wastewater Treatment Facilities
Direct Evidence for Deterministic Assembly of Bacterial Communities in Full-Scale Municipal Wastewater Treatment Facilities Open
Understanding the mechanisms of bacterial community assembly is one of the grand challenges of microbial ecology. In environmental systems, this challenge is exacerbated because replicate experiments are typically impossible; that is, micr…
View article: Different Engineering Designs Have Profoundly Different Impacts on the Microbiome and Nitrifying Bacterial Populations in Municipal Wastewater Treatment Bioreactors
Different Engineering Designs Have Profoundly Different Impacts on the Microbiome and Nitrifying Bacterial Populations in Municipal Wastewater Treatment Bioreactors Open
Municipal wastewater treatment facilities rely on the application of numerous “activated sludge” process designs to achieve site-specific treatment goals. A plethora of microbiome studies on municipal wastewater treatment bioreactors have …
View article: Seasonal Dynamics of the Activated Sludge Microbiome in Sequencing Batch Reactors, Assessed Using 16S rRNA Transcript Amplicon Sequencing
Seasonal Dynamics of the Activated Sludge Microbiome in Sequencing Batch Reactors, Assessed Using 16S rRNA Transcript Amplicon Sequencing Open
Sequencing batch reactors are a common design for wastewater treatment plants, particularly in smaller municipalities, due to their low footprint and ease of operations. However, like for most treatment plants in temperate/continental clim…
View article: Composition and Dynamics of the Activated Sludge Microbiome during Seasonal Nitrification Failure
Composition and Dynamics of the Activated Sludge Microbiome during Seasonal Nitrification Failure Open
Wastewater treatment plants in temperate climate zones frequently undergo seasonal nitrification failure in the winter month yet maintain removal efficiency for other contaminants. We tested the hypothesis that nitrification failure can be…
View article: Insights into Carbon Metabolism Provided by Fluorescence<i>In Situ</i>Hybridization-Secondary Ion Mass Spectrometry Imaging of an Autotrophic, Nitrate-Reducing, Fe(II)-Oxidizing Enrichment Culture
Insights into Carbon Metabolism Provided by Fluorescence<i>In Situ</i>Hybridization-Secondary Ion Mass Spectrometry Imaging of an Autotrophic, Nitrate-Reducing, Fe(II)-Oxidizing Enrichment Culture Open
The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter …
View article: Growth and Population Dynamics of the Anaerobic Fe(II)-Oxidizing and Nitrate-Reducing Enrichment Culture KS
Growth and Population Dynamics of the Anaerobic Fe(II)-Oxidizing and Nitrate-Reducing Enrichment Culture KS Open
Most isolated nitrate-reducing Fe(II)-oxidizing microorganisms are mixotrophic, meaning that Fe(II) is chemically oxidized by nitrite that forms during heterotrophic denitrification, and it is debated to which extent Fe(II) is enzymaticall…
View article: Insights into Nitrate-Reducing Fe(II) Oxidation Mechanisms through Analysis of Cell-Mineral Associations, Cell Encrustation, and Mineralogy in the Chemolithoautotrophic Enrichment Culture KS
Insights into Nitrate-Reducing Fe(II) Oxidation Mechanisms through Analysis of Cell-Mineral Associations, Cell Encrustation, and Mineralogy in the Chemolithoautotrophic Enrichment Culture KS Open
Most described nitrate-reducing Fe(II)-oxidizing bacteria (NRFeOB) are mixotrophic and depend on organic cosubstrates for growth. Encrustation of cells in Fe(III) minerals has been observed for mixotrophic NRFeOB but not for autotrophic ph…
View article: Nitrate capture and slow release in biochar amended compost and soil
Nitrate capture and slow release in biochar amended compost and soil Open
Slow release of nitrate by charred organic matter used as a soil amendment (i.e. biochar) was recently suggested as potential mechanism of nutrient delivery to plants which may explain some agronomic benefits of biochar. So far, isolated s…
View article: Gas entrapment and microbial N2O reduction reduce N2O emissions from a biochar-amended sandy clay loam soil
Gas entrapment and microbial N2O reduction reduce N2O emissions from a biochar-amended sandy clay loam soil Open
Nitrous oxide (N 2 O) is a potent greenhouse gas that is produced during microbial nitrogen transformation processes such as nitrification and denitrification. Soils represent the largest sources of N 2 O emissions with nitrogen fertilizer…
View article: Does soil aging affect the N<sub>2</sub>O mitigation potential of biochar? A combined microcosm and field study
Does soil aging affect the N<sub>2</sub>O mitigation potential of biochar? A combined microcosm and field study Open
The application of biochar as a soil amendment to improve soil fertility has been suggested as a tool to reduce soil‐borne CO 2 and non‐ CO 2 greenhouse gas emissions, especially nitrous oxide (N 2 O). Both laboratory and field trials have…
View article: A metagenomic-based survey of microbial (de)halogenation potential in a German forest soil
A metagenomic-based survey of microbial (de)halogenation potential in a German forest soil Open
In soils halogens (fluorine, chlorine, bromine, iodine) are cycled through the transformation of inorganic halides into organohalogen compounds and vice versa. There is evidence that these reactions are microbially driven but the key enzym…
View article: Erratum for Laufer et al., Coexistence of Microaerophilic, Nitrate-Reducing, and Phototrophic Fe(II) Oxidizers and Fe(III) Reducers in Coastal Marine Sediment
Erratum for Laufer et al., Coexistence of Microaerophilic, Nitrate-Reducing, and Phototrophic Fe(II) Oxidizers and Fe(III) Reducers in Coastal Marine Sediment Open
Volume 82, no. 5, p. 1433–1447, 2016. Page 1436, column 2, lines 51–52: “accession numbers LN986435 to LN986485” should be “accession numbers [LN896435][1] to [LN896485][2].”
Supplemental material, Table S2: Accession numbers LN986435 to …
View article: Metagenomic Analyses of the Autotrophic Fe(II)-Oxidizing, Nitrate-Reducing Enrichment Culture KS
Metagenomic Analyses of the Autotrophic Fe(II)-Oxidizing, Nitrate-Reducing Enrichment Culture KS Open
Nitrate-dependent ferrous iron [Fe(II)] oxidation (NDFO) is a well-recognized chemolithotrophic pathway in anoxic sediments. The neutrophilic chemolithoautotrophic enrichment culture KS originally obtained from a freshwater sediment (K. L.…
View article: Localized Plasticity in the Streamlined Genomes of Vinyl Chloride Respiring <em>Dehalococcoides</em>
Localized Plasticity in the Streamlined Genomes of Vinyl Chloride Respiring <em>Dehalococcoides</em> Open
Vinyl chloride (VC) is a human carcinogen and widespread priority pollutant. Here we report the first, to our knowledge, complete genome sequences of microorganisms able to respire VC, Dehalococcoides sp. strains VS and BAV1. Notably, the …
View article: Nitrate and ammonium concentration of compost, biochar-amended composts, soil and biochar-amended soil during repeated extractions
Nitrate and ammonium concentration of compost, biochar-amended composts, soil and biochar-amended soil during repeated extractions Open
Repeated extractions of nitrate and ammonium were performed on compost (Con), biochar-amended composts (CB1, CB2, CB3), soil and biochar-amended soil. Data presents the amount of N released during each step of extraction from each replicat…