Sebastian Bitsch
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View article: A Recognition Tag of Human Origin for Bioorthogonal Generation of Antibody‐Drug Conjugates using Microbial Biotin Ligase
A Recognition Tag of Human Origin for Bioorthogonal Generation of Antibody‐Drug Conjugates using Microbial Biotin Ligase Open
The use of enzymes, such as microbial transglutaminase, lipoate protein ligase A, or sortase A, for the generation of antibody‐drug conjugates has proven to be a powerful tool for the site‐specific payload conjugation to tumor‐specific ant…
View article: Evaluation of Potency and Specificity of Cryptophycin‐Loaded Antibody‐Drug Conjugates
Evaluation of Potency and Specificity of Cryptophycin‐Loaded Antibody‐Drug Conjugates Open
An enhanced variant of the antimitotic toxin cryptophycin was conjugated to the anti‐Her2 monoclonal antibody (mAb) Trastuzumab upon Michael addition. Either antibodies with freed hinge‐region cysteines or THIOMAB formats with engineered c…
View article: Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids
Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids Open
Here we report the generation of nanobody dextran polymer conjugates (dextraknobs) that are loaded with small molecules, i.e., fluorophores or photosensitizers, for potential applications in cancer diagnostics and therapy. To this end, the…
View article: Hell’s Kitchen: The Banquet in the Hereafter and the Reflexion of Zoroastrian Eschatological Motifs in the Qurʾān
Hell’s Kitchen: The Banquet in the Hereafter and the Reflexion of Zoroastrian Eschatological Motifs in the Qurʾān Open
This article discusses potential Zoroastrian prefigurations concerning the Qurʾānic imagination of tormenting and distasteful food in hell. Although research on paradise and hell in the Qurʾān and the Islamic tradition has recently undergo…
View article: Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase
Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase Open
Sactipeptides are ribosomally synthesized peptides containing a unique sulfur to α ‐carbon crosslink. Catalyzed by sactisynthases, this thioether pattern endows sactipeptides with enhanced structural, thermal, and proteolytic stability, wh…
View article: Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase
Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase Open
Substitution I253Q on a glycosylated IgG1 antibody allows microbial transglutaminase-mediated conjugation of a fluorophore or a clickable auristatin. The resulting antibody–drug conjugate showed excellent cell toxicity and no FcRn-mediated…
View article: ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells
ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells Open
Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of th…
View article: Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays Open
Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test dev…
View article: Multivalent dextran hybrids for efficient cytosolic delivery of biomolecular cargoes
Multivalent dextran hybrids for efficient cytosolic delivery of biomolecular cargoes Open
The development of novel biotherapeutics based on peptides and proteins is often limited to extracellular targets, because these molecules are not able to reach the cytosol. In recent years, several approaches were proposed to overcome thi…
View article: A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well‐Defined Protein–Protein Conjugates
A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well‐Defined Protein–Protein Conjugates Open
Bioorthogonal chemistry holds great potential to generate difficult‐to‐access protein–protein conjugate architectures. Current applications are hampered by challenging protein expression systems, slow conjugation chemistry, use of undesira…
View article: A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well‐Defined Protein–Protein Conjugates
A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well‐Defined Protein–Protein Conjugates Open
Bioorthogonal chemistry holds great potential to generate difficult‐to‐access protein–protein conjugate architectures. Current applications are hampered by challenging protein expression systems, slow conjugation chemistry, use of undesira…
View article: A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates
A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates Open
A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DAinv). With the aim of precisely generating branched protein chimeras, we syst…
View article: A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates
A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates Open
A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA inv ). With the aim of precisely generating branched protein chimeras, we sy…