Sebastian Triesch
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View article: Single-nuclei sequencing of <i>Moricandia arvensis</i> reveals bundle sheath cell function in the photorespiratory shuttle of C3-C4 intermediate Brassicaceae
Single-nuclei sequencing of <i>Moricandia arvensis</i> reveals bundle sheath cell function in the photorespiratory shuttle of C3-C4 intermediate Brassicaceae Open
Spatially confined gene expression determines cell identity and is fundamental to complex plant traits. In the evolutionary transition from C3 to the more efficient C4 photosynthesis, restricting the glycine decarboxylase reaction to bundl…
View article: A transcription factor module mediating C2 photosynthesis in the Brassicaceae
A transcription factor module mediating C2 photosynthesis in the Brassicaceae Open
C 4 photosynthesis has arisen from the ancestral C 3 state in over sixty lineages of angiosperms. It is widely accepted that an early step in C 4 evolution is associated with the appearance of so-called C 2 photosynthesis caused by loss of…
View article: Single-nuclei sequencing of<i>Moricandia arvensis</i>reveals bundle sheath cell function in the photorespiratory shuttle of C<sub>3</sub>-C<sub>4</sub>intermediate Brassicaceae
Single-nuclei sequencing of<i>Moricandia arvensis</i>reveals bundle sheath cell function in the photorespiratory shuttle of C<sub>3</sub>-C<sub>4</sub>intermediate Brassicaceae Open
Spatially confined gene expression determines cell identity and is fundamental to complex plant traits. In the evolutionary transition from C 3 to the more efficient C 4 photosynthesis, restricting the glycine decarboxylase reaction to bun…
View article: Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species
Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species Open
C 3 ‐C 4 intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking bona fide C 4 species. The establishment of this carbon concentrating mechanism is known to require a comple…
View article: Predmoter—cross-species prediction of plant promoter and enhancer regions
Predmoter—cross-species prediction of plant promoter and enhancer regions Open
Motivation Identifying cis-regulatory elements (CREs) is crucial for analyzing gene regulatory networks. Next generation sequencing methods were developed to identify CREs but represent a considerable expenditure for targeted analysis of f…
View article: Predmoter - Cross-species prediction of plant promoter and enhancer regions
Predmoter - Cross-species prediction of plant promoter and enhancer regions Open
Motivation The identification of cis -regulatory elements (CREs) is crucial for the analysis of gene regulatory networks in plants. Several next generation sequencing (NGS)-based methods were developed to identify CREs. However, these meth…
View article: A transcription factor module mediating C<sub>2</sub>photosynthesis
A transcription factor module mediating C<sub>2</sub>photosynthesis Open
C 4 photosynthesis has arisen from the ancestral C 3 state in over sixty lineages of angio-sperms. It is widely accepted that an early step in C 4 evolution is restriction of glycine decarboxylase activity to bundle sheath cells to generat…
View article: A genomic panel for studying C3‐C4 intermediate photosynthesis in the Brassiceae tribe
A genomic panel for studying C3‐C4 intermediate photosynthesis in the Brassiceae tribe Open
Research on C4 and C3‐C4 photosynthesis has attracted significant attention because the understanding of the genetic underpinnings of these traits will support the introduction of its characteristics into commercially relevant crop species…
View article: A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803
A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803 Open
Cyanobacteria are a promising platform for the production of the triterpene squalene (C30), a precursor for all plant and animal sterols, and a highly attractive intermediate towards triterpenoids, a large group of secondary plant metaboli…
View article: Helixer–<i>de novo</i>Prediction of Primary Eukaryotic Gene Models Combining Deep Learning and a Hidden Markov Model
Helixer–<i>de novo</i>Prediction of Primary Eukaryotic Gene Models Combining Deep Learning and a Hidden Markov Model Open
A bstract Gene structural annotation is a critical step in obtaining biological knowledge from genome sequences yet remains a major challenge in genomics projects. Current de novo Hidden Markov Models are limited in their capacity to model…
View article: A genomic panel for studying C3-C4 intermediate photosynthesis in the Brassiceae tribe
A genomic panel for studying C3-C4 intermediate photosynthesis in the Brassiceae tribe Open
Research on C4 and C3-C4 photosynthesis has attracted significant attention because the understanding of the genetic underpinnings of this trait will support the introduction of its characteristics into commercially relevant crop species. …
View article: Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species
Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species Open
C 3 -C 4 intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking bona fide C 4 species. The establishment of this carbon concentrating mechanism is known to require a comple…
View article: Squalene Quantification using Nile Red Staining (Developmental) v1
Squalene Quantification using Nile Red Staining (Developmental) v1 Open
This protocol is under development! Nile Red is a fluorescent dye that stains selectively hydrophobic substances. We assume that squalene accumulates in the cell membrane or in lipid vesicles where it can be stained by the dye.
View article: First strand cDNA synthesis (ThermoScientific RevertAid) v1
First strand cDNA synthesis (ThermoScientific RevertAid) v1 Open
The following protocol is optimized to generate first-strand cDNA for use in two step-PCR.
View article: Squalene Quantification using Nile Red Staining (M4455 Version) v1
Squalene Quantification using Nile Red Staining (M4455 Version) v1 Open
This protocol is under development and for teaching purposes only! Nile Red is a fluorescent dye that stains selectively hydrophobic substances. We assume that squalene accumulates in the cell membrane or in lipid vesicles where it can be …
View article: Extraction and Lowry-Assay for determination of Synechocystis total protein v1
Extraction and Lowry-Assay for determination of Synechocystis total protein v1 Open
A quick Lowry.Assay for the extraction and determination of total protein from Synechocystis. Photo credit: Miriam Dreesbach, Institute for Synthetic Microbiology, HHU Düsseldorf
View article: Phycocyanin Extraction from Synechocystis v1
Phycocyanin Extraction from Synechocystis v1 Open
Extraction of Phycocyanin from Synechocystis liquid culture.