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Enhancer-driven random gene overexpression (ERGO): a method to study gene function in Chlamydomonas Open
Gene overexpression can be used to study gene function and is more suitable to characterize essential and redundant genes than gene knockout. A forward genetic approach based on random gene overexpression, also known as activation tagging …
Extensive remodeling during <i>Chlamydomonas reinhardtii</i> zygote maturation leads to highly resistant zygospores Open
SUMMARY The unicellular soil alga Chlamydomonas reinhardtii forms diploid zygotes during its sexual cycle. The process of a zygote maturing into a highly resistant zygospore remains poorly understood despite its importance for survival und…
Characterization of activating <i>cis</i>‐regulatory elements from the histone genes of <i>Chlamydomonas reinhardtii</i> Open
SUMMARY Regulation of gene expression in eukaryotes is controlled by cis ‐regulatory modules (CRMs). A major class of CRMs are enhancers which are composed of activating cis ‐regulatory elements (CREs) responsible for upregulating transcri…
View article: A mutualistic bacterium rescues a green alga from an antagonist
A mutualistic bacterium rescues a green alga from an antagonist Open
Photosynthetic protists, known as microalgae, are key contributors to primary production on Earth. Since early in evolution, they coexist with bacteria in nature, and their mode of interaction shapes ecosystems. We have recently shown that…
Reciprocal modulation of responses to nitrate starvation and hypoxia in roots and leaves of <i>Arabidopsis thaliana</i> Open
The flooding of agricultural land leads to hypoxia and nitrate leaching. While understanding the plant's response to these conditions is essential for crop improvement, the effect of extended nitrate limitation on subsequent hypoxia has no…
Reciprocal modulation of responses to nitrate starvation and hypoxia in roots and leaves of <i>Arabidopsis thaliana</i> Open
The flooding of agricultural land leads to hypoxia and nitrate leaching. While understanding the plant’s response to these conditions is essential for crop improvement, the effect of extended nitrate limitation on subsequent hypoxia has no…
Front Cover: Characterization of an Unusual α‐Oxoamine Synthase Off‐Loading Domain from a Cyanobacterial Type I Fatty Acid Synthase (ChemBioChem 18/2023) Open
A new fatty acid synthase off-loading domain. Fatty acid synthase (FAS) usually releases its products by hydrolysis or transesterification. Here, an α-oxoamine synthase off-loading domain is identified in a cyanobacterial type I FAS. This …
Not All Acidovorax Are Created Equal: Gibberellin Biosynthesis in the Turfgrass Pathogen <i>Acidovorax avenae</i> subsp. <i>avenae</i> Open
In recent years Acidovorax avenae subsp. avenae was identified as a major cause of bacterial etiolation and decline (BED) in turfgrasses and has become a growing economical concern for the turfgrass industry. The symptoms of BED resemble t…
Characterization of an Unusual α‐Oxoamine Synthase Off‐Loading Domain from a Cyanobacterial Type I Fatty Acid Synthase Open
Type I fatty acid synthases (FASs) are known from higher eukaryotes and fungi. We report the discovery of FasT, a rare type I FAS from the cyanobacterium Chlorogloea sp. CCALA695. FasT possesses an unusual off‐loading domain, which was het…
<span><i>Chlamydomonas reinhardtii</i></span> mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress Open
Old Yellow Enzymes (OYEs) are flavin‐containing ene‐reductases that have been intensely studied with regard to their biotechnological potential for sustainable chemical syntheses. OYE‐encoding genes are found throughout the domains of life…
The leaf is always greener on the other side of the lab: Optical in-situ indicators for leaf chlorophyll content need improvement for semi-natural grassland areas Open
Leaf chlorophyll content (LCC) is an important indicator of plant health. Earth observation facilitates LCC monitoring on large spatial scales and within short time intervals by retrieving canopy LCC from spectral signals. However, in-situ…
Deciphering Chemical Mediators Regulating Specialized Metabolism in a Symbiotic Cyanobacterium Open
Genomes of cyanobacteria feature a variety of cryptic biosynthetic pathways for complex natural products, but the peculiarities limiting the discovery and exploitation of the metabolic dark matter are not well understood. Here we describe …
Bacterial marginolactones trigger formation of algal gloeocapsoids, protective aggregates on the verge of multicellularity Open
Photosynthetic microorganisms including the green alga Chlamydomonas reinhardtii are essential to terrestrial habitats as they start the carbon cycle by conversion of CO 2 to energy-rich organic carbohydrates. Terrestrial habitats are dens…
A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga Open
Significance Algae live in association with microbes that interact by a variety of chemical mediators, resulting in mutualistic or antagonistic relationships. Although algae are key contributors to carbon fixation and are fundamental for f…
The bacterium <i>Pseudomonas protegens</i> antagonizes the microalga <span> <i>Chlamydomonas reinhardtii</i> </span> using a blend of toxins Open
Summary The unicellular alga Chlamydomonas reinhardtii and the bacterium Pseudomonas protegens serve as a model to study the interactions between photosynthetic and heterotrophic microorganisms. P . protegens secretes the cyclic lipopeptid…
A polyyne toxin produced by an antagonistic bacterium blinds and lyses a green microalga Open
Microalgae are key contributors to global carbon fixation and the basis of many food webs. In nature, their growth is often supported or suppressed by other microorganisms. The bacterium Pseudomonas protegens Pf-5 arrests the growth of the…
Decision letter: Quantitative insights into the cyanobacterial cell economy Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Phototrophic microorganisms are promising …
From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in nature Open
In the mid-20th century, the unicellular and genetically tractable green alga Chlamydomonas reinhardtii was first developed as a model organism to elucidate fundamental cellular processes such as photosynthesis, light perception and the st…
A giant type I polyketide synthase participates in zygospore maturation in <i>Chlamydomonas reinhardtii</i> Open
Summary Polyketide synthases ( PKS s) occur in many bacteria, fungi and plants. They are highly versatile enzymes involved in the biosynthesis of a large variety of compounds including antimicrobial agents, polymers associated with bacteri…
A Plant Cryptochrome Controls Key Features of the <i>Chlamydomonas</i> Circadian Clock and Its Life Cycle Open
Cryptochromes are flavin-binding proteins that act as blue light receptors in bacteria, fungi, plants, and insects and are components of the circadian oscillator in mammals. Animal and plant cryptochromes are evolutionarily divergent, alth…
Use of Fibonacci numbers in lipidomics – Enumerating various classes of fatty acids Open
In lipid biochemistry, a fundamental question is how the potential number of fatty acids increases with their chain length. Here, we show that it grows according to the famous Fibonacci numbers when cis / trans isomerism is neglected. Sinc…
Research data supporting "Cyanobacteria and eukaryotic algae use different chemical variants of vitamin B12" Open
Data supporting the publication "Cyanobacteria and eukaryotic algae use different chemical variants of vitamin B12" A full description of the data in found in each of the datafiles.