Shanjiang Zhao
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View article: The status of timed artificial insemination technology in large-scale Chinese dairy farms
The status of timed artificial insemination technology in large-scale Chinese dairy farms Open
Here, we aim to review the evolution of timed artificial insemination (TAI) technology in large-scale dairy farms in China and provide experience and reference guidelines for the improvement of reproductive efficiency in such farms. We use…
View article: Obesity and oxidative stress: Implications for female fertility
Obesity and oxidative stress: Implications for female fertility Open
Obesity has reached epidemic proportions in most parts of the world, and it is estimated that 1 billion people globally are trapped in an obesity pandemic, which has seriously compromised human health. Recently, there has been a flood of r…
View article: Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells
Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells Open
View article: Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells
Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells Open
Background: Negative energy balance (NEB) typically occurs in dairy cows after delivery, with those in high yielding being more likely to experience significant NEB. Such metabolic imbalance could cause ketosis, which is often accompanied …
View article: Phenotypic and Genetic Analyses of In Vitro Embryo Production Traits in Chinese Holstein Cattle
Phenotypic and Genetic Analyses of In Vitro Embryo Production Traits in Chinese Holstein Cattle Open
Ovum pick up and in vitro embryo production (OPU-IVEP) is an essential technique in the dairy industry. The production efficiency of OPU-IVEP is significantly influenced by various factors, and phenotypic and genetic characteristics are hi…
View article: Unveiling the Transcriptomic Landscape of Holstein Bull Testes Post-Sexual Maturation: Insights into Spermatogenesis-Associated MicroRNAs and mRNA Profiles
Unveiling the Transcriptomic Landscape of Holstein Bull Testes Post-Sexual Maturation: Insights into Spermatogenesis-Associated MicroRNAs and mRNA Profiles Open
Spermatogenesis is a complex, tightly regulated biological process crucial for male fertility in mammals. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) play essential functions in regulating gene expression during spermatogenesis, and thei…
View article: Unveiling the Transcriptomic Landscape of Holstein Bull Testes Post-Sexual Maturation: Insights into Spermatogenesis-Associated MicroRNAs and mRNA Profiles
Unveiling the Transcriptomic Landscape of Holstein Bull Testes Post-Sexual Maturation: Insights into Spermatogenesis-Associated MicroRNAs and mRNA Profiles Open
Spermatogenesis is a complex, tightly regulated biological process crucial for male fertility in mammals. MicroRNAs (miRNAs) and messenger RNAs (mRNAs) play essential functions in regulating gene expression during spermatogenesis, and thei…
View article: Sirtuin 3 regulation: a target to alleviate β-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells
Sirtuin 3 regulation: a target to alleviate β-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells Open
View article: Genetic Polymorphism and mRNA Expression Studies Reveal IL6R and LEPR Gene Associations with Reproductive Traits in Chinese Holsteins
Genetic Polymorphism and mRNA Expression Studies Reveal IL6R and LEPR Gene Associations with Reproductive Traits in Chinese Holsteins Open
Genetic selection of milk yield traits alters the energy distribution of high producing cows, resulting in gene-induced negative energy balance, and consequently, poor body condition scores and reduced reproductive performances. Here, we i…
View article: Bioinformatics Analysis of Circrna Expression and Construction of "Circrna-Mirna-Mrna" Competing Endogenous Rnas Networks of Spermatogenesis in Post-Sexual Maturation Testes of Holstein Bulls
Bioinformatics Analysis of Circrna Expression and Construction of "Circrna-Mirna-Mrna" Competing Endogenous Rnas Networks of Spermatogenesis in Post-Sexual Maturation Testes of Holstein Bulls Open
View article: Uncovering Novel Features of the Pc Locus in Horn Development from Gene-Edited Holstein Cattle by RNA-Sequencing Analysis
Uncovering Novel Features of the Pc Locus in Horn Development from Gene-Edited Holstein Cattle by RNA-Sequencing Analysis Open
The Polled Celtic (Pc) mutation locus is a genetically simple single mutation that is the best choice for breeding polled cattle using gene editing. However, the mechanism of the Pc locus for regulating horn development is unclear, so we u…
View article: Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs
Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs Open
Correct reprogramming of the DLK1-DIO3 imprinted region is critical for the development of cloned animals. However, in pigs, the imprinting and regulation of the DLK1 - DIO3 region has not been systematically analyzed. The objective of thi…
View article: The Dynamic Transcription Profiles of Proliferating Bovine Ovarian Granulosa When Exposed to Increased Levels of β-Hydroxybutyric Acid
The Dynamic Transcription Profiles of Proliferating Bovine Ovarian Granulosa When Exposed to Increased Levels of β-Hydroxybutyric Acid Open
Ketosis is common in high-yield dairy cows. It is a condition that is characterized by the accumulation of serum β-hydroxybutyric acid (BHBA). Both subclinical ketosis and clinical ketosis can compromise the reproductive performance and ca…
View article: Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine
Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine Open
O-linked β-N-acetylglucosamine (O-GlcNAc) modification is a ubiquitous, reversible, and highly dynamic post-translational modification, which takes charge of almost all biological processes examined. However, little information is availabl…
View article: Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System
Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System Open
Background: Insulin-like growth factor 2 is a growth-promoting factor that plays an important role in the growth and development of mammals. A nucleotide substitution in intron 3 of IGF2—which disrupts the ZBED6-binding site—affects muscle…
View article: Mitofusins: from mitochondria to fertility
Mitofusins: from mitochondria to fertility Open
Germ cell formation and embryonic development require ATP synthesized by mitochondria. The dynamic system of the mitochondria, and in particular, the fusion of mitochondria, are essential for the generation of energy. Mitofusin1 and mitofu…
View article: Joint Transcriptome and Metabolome Analysis Prevails the Biological Mechanisms Underlying the Pro-Survival Fight in In Vitro Heat-Stressed Granulosa Cells
Joint Transcriptome and Metabolome Analysis Prevails the Biological Mechanisms Underlying the Pro-Survival Fight in In Vitro Heat-Stressed Granulosa Cells Open
Previous studies reported the physical, transcriptome, and metabolome changes in in vitro acute heat-stressed (38 °C versus 43 °C for 2 h) bovine granulosa cells. Granulosa cells exhibited transient proliferation senescence, oxidative stre…
View article: Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts
Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts Open
The quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate…
View article: Characterization of the Microbial Communities along the Gastrointestinal Tract in Crossbred Cattle
Characterization of the Microbial Communities along the Gastrointestinal Tract in Crossbred Cattle Open
The gastrointestinal microbiota greatly affects the health status and production performance of bovines. Presently, many studies have used high-throughput sequencing methods to investigate the gastrointestinal microbiome in bovines. Howeve…
View article: Investigation of Metabolome Underlying the Biological Mechanisms of Acute Heat Stressed Granulosa Cells
Investigation of Metabolome Underlying the Biological Mechanisms of Acute Heat Stressed Granulosa Cells Open
Heat stress affects granulosa cells and the ovarian follicular microenvironment, ultimately resulting in poor oocyte developmental competence. This study aims to investigate the metabo-lomics response of bovine granulosa cells (bGCs) to in…
View article: Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets
Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets Open
Long noncoding RNAs (lncRNAs) are composed of nucleotides located in the nucleus and cytoplasm; these are transcribed by RNA polymerase II and are greater than 200 nt in length. LncRNAs fulfill important functions in a variety of biologica…
View article: Long noncoding RNAs: new insights in modulating mammalian spermatogenesis
Long noncoding RNAs: new insights in modulating mammalian spermatogenesis Open
View article: Effects of Inhibin A on Apoptosis and Proliferation of Bovine Granulosa Cells
Effects of Inhibin A on Apoptosis and Proliferation of Bovine Granulosa Cells Open
Inhibin A is well known for its inhibitory properties against follicle-stimulating hormone (FSH), released through a pituitary–gonadal negative feedback loop to regulate follicular development. Ovarian folliculogenesis, hormonal biosynthes…
View article: Thioredoxin-interacting protein regulates glucose metabolism and improves the intracellular redox state in bovine oocytes during in vitro maturation
Thioredoxin-interacting protein regulates glucose metabolism and improves the intracellular redox state in bovine oocytes during in vitro maturation Open
The extent of glucose metabolism during oocyte maturation is closely related to oocyte developmental potential. Thioredoxin-interacting protein (TXNIP) is an α-arrestin family protein that negatively regulates glucose uptake into cells. Ho…
View article: Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Open
Anti-Mullerian hormone (AMH) is an important reproductive marker of ovarian reserve produced by granulosa cells (GCs) of pre-antral and early-antral ovarian follicles in several species, including cattle. This hormone plays a vital role du…
View article: Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1 Open
Little information is available regarding the effect of melatonin on the quality and fertilization capability of sex-sorted bull sperm, and even less about the associated mechanism. Sex-sorted sperm from three individual bulls were washed …
View article: No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas
No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas Open
View article: Detrimental effects of lipopolysaccharides on maturation of bovine oocytes
Detrimental effects of lipopolysaccharides on maturation of bovine oocytes Open
Together, these results provide an underlying mechanism by which LPS impairs maturation potential in bovine oocytes.
View article: Beneficial role of melatonin in protecting mammalian gametes and embryos from oxidative damage
Beneficial role of melatonin in protecting mammalian gametes and embryos from oxidative damage Open
View article: Effects of lipopolysaccharide on maturation of bovine oocyte <i>in vitro</i> and its possible mechanisms
Effects of lipopolysaccharide on maturation of bovine oocyte <i>in vitro</i> and its possible mechanisms Open
Lipopolysaccharide disturbs the secretion of gonadotropin, endometrial function and implantation efficiency. However, there is little information regarding the effects of lipopolysaccharide on cyclic ovary activity, especially oocyte matur…