Shaoji Cheng
YOU?
Author Swipe
View article: Emergence of <i>mutH</i> <sup>V76G</sup> among longitudinal carbapenem resistant <i>Klebsiella pneumoniae</i> causing long-term colonization and recurrent infection disrupts DNA mismatch repair and results in a hypermutator phenotype
Emergence of <i>mutH</i> <sup>V76G</sup> among longitudinal carbapenem resistant <i>Klebsiella pneumoniae</i> causing long-term colonization and recurrent infection disrupts DNA mismatch repair and results in a hypermutator phenotype Open
Although hypermutation due to Mut protein mutations that disrupt DNA mismatch repair has been characterized in some bacteria, its mechanisms and consequences in Klebsiella pneumoniae remain poorly defined. We analyzed 11 longitudinal KPC-3…
View article: Medical Cannabis as the Source of <i>Cryptococcus neoformans</i> Infection
Medical Cannabis as the Source of <i>Cryptococcus neoformans</i> Infection Open
Whole-genome sequencing demonstrated that Cryptococcus neoformans serotype A from cerebrospinal fluid and larynx of a patient receiving therapy for refractory multiple myeloma and from the patient's medical cannabis were indistinguishable.…
View article: P-1018. A comprehensive strategy identifies <i>Candida albicans</i> genes and biologic processes that contribute to pathogenesis of intra-abdominal candidiasis (IAC)
P-1018. A comprehensive strategy identifies <i>Candida albicans</i> genes and biologic processes that contribute to pathogenesis of intra-abdominal candidiasis (IAC) Open
Background IAC pathogenesis is poorly understood.Figure 1.C. albicans transcription factor mutants attenuated during IAC. Methods We used RNA-Seq to measure C. albicans SC5314 gene expression during early peritonitis (30-min), late periton…
View article: 96. <i>Candida albicans</i> bloodstream infections are comprised of diverse populations of strains, including antifungal tolerant strains that emerge during treatment failure
96. <i>Candida albicans</i> bloodstream infections are comprised of diverse populations of strains, including antifungal tolerant strains that emerge during treatment failure Open
Background The longstanding model is that most bloodstream infections (BSIs) are caused by a single strain that passes through a bottleneck. C. albicans exhibits genomic and phenotypic plasticity, but it is unknown whether within-patient (…
View article: P-353. <i>Aspergillus</i> Burdens in Outdoor and Indoor Air at Hospitals Housing Patients at High Risk for Invasive Fungal Infections
P-353. <i>Aspergillus</i> Burdens in Outdoor and Indoor Air at Hospitals Housing Patients at High Risk for Invasive Fungal Infections Open
Background Airborne dissemination of conidia is implicated in transmission of pathogenic fungi. There is lack of knowledge on airborne fungal burdens in hospital environments. Methods Air at hospitals A and B (1.5 miles apart) was sampled …
View article: P-249. Diverse populations of viable pathogenic moulds and <i>Candida</i>, including <i>C. auris</i>, are commonly recovered from air samples at two tertiary care hospitals
P-249. Diverse populations of viable pathogenic moulds and <i>Candida</i>, including <i>C. auris</i>, are commonly recovered from air samples at two tertiary care hospitals Open
Background Airborne fungi are important nosocomial pathogens. However, there are scant data on populations of viable fungi in air from hospital environments.Table 1.Hospitals and units included in air sampling Methods We cultured air sampl…
View article: Blood cultures contain populations of genetically diverse<i>Candida albicans</i>strains that may differ in echinocandin tolerance and virulence
Blood cultures contain populations of genetically diverse<i>Candida albicans</i>strains that may differ in echinocandin tolerance and virulence Open
It is unknown whether within-patient Candida albicans diversity is common during bloodstream infections (BSIs). We determined whole genome sequences of 10 C. albicans strains from blood cultures (BCs) in each of 4 patients. BCs in 3 patien…
View article: 1422. Surveillance for fungi in hospital environments housing high-risk hosts using culture and culture-independent methods
1422. Surveillance for fungi in hospital environments housing high-risk hosts using culture and culture-independent methods Open
Background Nosocomial outbreaks of fungal infections are increasingly reported. There are scant data on fungal burdens in hospital environments. Standardized surveillance methods are lacking. Methods We developed culture and culture-indepe…
View article: Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures
Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures Open
The longstanding paradigm is that most bloodstream infections (BSIs) are caused by a single organism. We performed whole genome sequencing of five-to-ten strains from blood culture (BC) bottles in each of ten patients with Candida glabrata…
View article: 1837. Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem Resistant <i>Klebsiella pneumoniae</i> from Blood Cultures of Individual Patients
1837. Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem Resistant <i>Klebsiella pneumoniae</i> from Blood Cultures of Individual Patients Open
Background The longstanding paradigm is that almost all bloodstream infections (BSIs) stem from a single, clonal organism. We hypothesized that carbapenem resistant K. pneumoniae (CRKP) from individual patients (pts) with BSIs are genetica…
View article: Within-Host Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem-Resistant Klebsiella pneumoniae from Blood Cultures of Patients with Bacteremia
Within-Host Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem-Resistant Klebsiella pneumoniae from Blood Cultures of Patients with Bacteremia Open
The long-standing paradigm for pathogenesis of bacteremia is that, in most cases, a single organism passes through a bottleneck and establishes itself in the bloodstream (single-organism hypothesis). In keeping with this paradigm, standard…
View article: Within-host genotypic and phenotypic diversity of contemporaneous carbapenem-resistant <i>Klebsiella pneumoniae</i> from blood cultures of patients with bacteremia
Within-host genotypic and phenotypic diversity of contemporaneous carbapenem-resistant <i>Klebsiella pneumoniae</i> from blood cultures of patients with bacteremia Open
Carbapenem-resistant Klebsiella pneumoniae (CRKP) are major pathogens globally. It is unknown whether bloodstream infections (BSIs) by CRKP and other bacteria are commonly caused by single organisms or mixed microbial populations. We hypot…
View article: Different Conformations Revealed by NMR Underlie Resistance to Ceftazidime/Avibactam and Susceptibility to Meropenem and Imipenem among D179Y Variants of KPC β-Lactamase
Different Conformations Revealed by NMR Underlie Resistance to Ceftazidime/Avibactam and Susceptibility to Meropenem and Imipenem among D179Y Variants of KPC β-Lactamase Open
β-Lactamase-mediated resistance to ceftazidime-avibactam (CZA) is a serious limitation in the treatment of Gram-negative bacteria harboring Klebsiella pneumoniae carbapenemase (KPC). Herein, the basis of susceptibility to carbapenems and r…
View article: Candida glabrata MSH2 deletion increases antifungal tolerance in vitro and during intra-abdominal candidiasis (IAC), it does not impact pathogenesis of peritonitis or intra-abdominal abscesses
Candida glabrata MSH2 deletion increases antifungal tolerance in vitro and during intra-abdominal candidiasis (IAC), it does not impact pathogenesis of peritonitis or intra-abdominal abscesses Open
Background: IAC is the second most common type of invasive Candidiasis, but its pathogenesis is poorly understood. We have shown that Candida albicans DNA damage response genes are strongly induced within intra-abdominal abscesses. Deletio…
View article: 215. <i>Candida glabrata</i> (<i>CG</i>) Bloodstream Infections (BSIs) Are Characterized by Genetically Diverse Populations of Strain Variants Recovered from Positive Blood Cultures (BCs)
215. <i>Candida glabrata</i> (<i>CG</i>) Bloodstream Infections (BSIs) Are Characterized by Genetically Diverse Populations of Strain Variants Recovered from Positive Blood Cultures (BCs) Open
Background The long-standing paradigm is that almost all BSIs stem from a single, clonal organism (“single-organism” hypothesis). We hypothesized that CG-positive BCs were comprised of genetically diverse strain variants. Methods Five to t…
View article: 1007. Hypermutability in Clinical Strains of <i>Klebsiella pneumoniae</i>: Role of the V76G Mutation in MutH
1007. Hypermutability in Clinical Strains of <i>Klebsiella pneumoniae</i>: Role of the V76G Mutation in MutH Open
Background Hypermutator (HM) bacteria exhibit high spontaneous mutation rates due to DNA mismatch repair (MMR) gene mutations, which may facilitate antibiotic resistance. HM is best described for chronic infections or colonization, in part…
View article: 116. Characterization of small colony variants from a patient with bloodstream infection of Candida glabrata
116. Characterization of small colony variants from a patient with bloodstream infection of Candida glabrata Open
Background Bacterial small colony variants (SCVs) that are tolerant to commonly used antibiotics are well recognized. Clinical SCV Candida have been rarely reported. We describe SCV C. glabrata (CG) strains recovered from within a populati…
View article: Evaluation of a high-throughput, cost-effective Illumina library preparation kit
Evaluation of a high-throughput, cost-effective Illumina library preparation kit Open
Library preparation for high-throughput sequencing applications is a critical step in producing representative, unbiased sequencing data. The iGenomX Riptide High Throughput Rapid Library Prep Kit purports to provide high-quality sequencin…
View article: Molecular Epidemiology, Natural History, and Long-Term Outcomes of Multidrug-Resistant Enterobacterales Colonization and Infections Among Solid Organ Transplant Recipients
Molecular Epidemiology, Natural History, and Long-Term Outcomes of Multidrug-Resistant Enterobacterales Colonization and Infections Among Solid Organ Transplant Recipients Open
Background Multidrug-resistant Enterobacterales (MDR-E), including carbapenem-resistant and third-generation cephalosporin-resistant Enterobacterales (CRE, CefR-E), are major pathogens following solid organ transplantation (SOT). Methods W…
View article: Genetic diversity of clinical and environmental Mucorales isolates obtained from an investigation of mucormycosis cases among solid organ transplant recipients
Genetic diversity of clinical and environmental Mucorales isolates obtained from an investigation of mucormycosis cases among solid organ transplant recipients Open
Mucormycoses are invasive infections by Rhizopus species and other Mucorales. Over 10 months, four solid organ transplant (SOT) recipients at our centre developed mucormycosis due to Rhizopus microsporus ( n =2), R. arrhizus ( n =1) or Lic…
View article: 2887. Identifying Candida albicans Transcription Factors (TFs) That Regulate Pathogenesis of Intra-abdominal Candidiasis (IAC) by Screening a Deletion Mutant Library
2887. Identifying Candida albicans Transcription Factors (TFs) That Regulate Pathogenesis of Intra-abdominal Candidiasis (IAC) by Screening a Deletion Mutant Library Open
Background IAC is a common manifestation of invasive candidiasis, but its pathogenesis is poorly understood. We developed a mouse model of C. albicans IAC, in which disease progresses from peritonitis to abscesses (IAA) in a manner that re…
View article: 246. Carbapenem-resistant Klebsiella (CRK) Bloodstream Infections (BSIs) Are Caused by Bacterial Populations That Are Genotypically and Phenotypically Diverse
246. Carbapenem-resistant Klebsiella (CRK) Bloodstream Infections (BSIs) Are Caused by Bacterial Populations That Are Genotypically and Phenotypically Diverse Open
Background The majority of bacterial BSIs are believed to stem from a single, clonal organism. We hypothesized that most CRK BSIs are caused by genetically diverse, clonal strains that exhibit different phenotypes. Methods Blood cultures (…
View article: 1726. Candida albicans Virulence Genes Induced During Intra-abdominal Candidiasis (IAC) in the Absence of Antifungal Exposure Mediate Echinocandin Resistance
1726. Candida albicans Virulence Genes Induced During Intra-abdominal Candidiasis (IAC) in the Absence of Antifungal Exposure Mediate Echinocandin Resistance Open
Background We developed and validated a mouse model of C. albicans IAC that mimics peritonitis and abscesses (IAA) in humans, and that is amenable to temporal–spatial transcriptional profiling and virulence studies. Methods We measured C. …
View article: 383. An Increased Rate of <i>Candida parapsilosis</i> Infective Endocarditis Is Associated With Injection Drug Use
383. An Increased Rate of <i>Candida parapsilosis</i> Infective Endocarditis Is Associated With Injection Drug Use Open
BACKGROUND: Candida parapsilosis fungemia typically occurs in patients with intravascular catheters or prosthetic devices. In 2017, we noted an increase in C. parapsilosis infective endocarditis (IE). METHODS: We retrospectively reviewed C…
View article: Ceftolozane-Tazobactam for the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Infections: Clinical Effectiveness and Evolution of Resistance
Ceftolozane-Tazobactam for the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Infections: Clinical Effectiveness and Evolution of Resistance Open
In this small study, ceftolozane-tazobactam was successful in treating 71% of patients with MDR-P. aeruginosa infections, most of whom had pneumonia. The emergence of ceftolozane-tazobactam resistance in 3 patients is worrisome and may be …
View article: Mutations in<i>bla</i><sub>KPC-3</sub>That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases
Mutations in<i>bla</i><sub>KPC-3</sub>That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases Open
We identified four bla KPC-3 mutations in ceftazidime-avibactam-resistant clinical Klebsiella pneumoniae isolates, corresponding to D179Y, T243M, D179Y/T243M, and EL165-166 KPC-3 variants. Using site-directed mutagenesis and transforming v…
View article: Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne <i>bla</i> <sub>KPC-3</sub> Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections
Emergence of Ceftazidime-Avibactam Resistance Due to Plasmid-Borne <i>bla</i> <sub>KPC-3</sub> Mutations during Treatment of Carbapenem-Resistant Klebsiella pneumoniae Infections Open
Ceftazidime-avibactam is a novel β-lactam/β-lactamase inhibitor with activity against carbapenem-resistant Enterobacteriaceae (CRE) that produce Klebsiella pneumoniae carbapenemase (KPC). We report the first cases of ceftazidime-avibactam …