Sharif Hala
YOU?
Author Swipe
View article: Genomic diversity and antimicrobial resistance of Staphylococcus aureus in Saudi Arabia: a nationwide study using whole-genome sequencing
Genomic diversity and antimicrobial resistance of Staphylococcus aureus in Saudi Arabia: a nationwide study using whole-genome sequencing Open
Methicillin-resistant Staphylococcus aureus (MRSA) surveillance in regions with mass gatherings presents unique challenges for public health systems. Saudi Arabia, hosting millions of pilgrims annually, provides a distinctive setting for s…
View article: Effect of guideline-concordant, appropriate, and effective antimicrobial treatment on patient outcome in multidrug-resistant (MDR) infections
Effect of guideline-concordant, appropriate, and effective antimicrobial treatment on patient outcome in multidrug-resistant (MDR) infections Open
Recently, to achieve cure, physicians have been resorting to overuse or misuse of antimicrobials to treat resistant infections, leading to the emergence of further resistant organisms. To overcome this issue, antimicrobial guidelines have …
View article: Molecular evolutionary insights into the repeated introductions and cryptic transmission of dengue virus in Saudi Arabia
Molecular evolutionary insights into the repeated introductions and cryptic transmission of dengue virus in Saudi Arabia Open
Our findings highlight Saudi Arabia's evolving role as a regional hub for DENV transmission, driven by mass gatherings and labor migration. Strengthening genomic surveillance, enhancing vector control, and fostering regional data sharing a…
View article: Effect of mineral fertilization and native microbial preparations on productivity of Borlotti beans (Phaseolus vulgaris)
Effect of mineral fertilization and native microbial preparations on productivity of Borlotti beans (Phaseolus vulgaris) Open
In order to fully realizing the potential of beneficial microorganisms in the agricultural system, there remains a huge research gap in understanding the factors shaping their functional activities in the rhizosphere. Recently, native micr…
View article: Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity
Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity Open
View article: Prevalence of Peritonitis in Continuous Ambulatory Peritoneal Dialysis Patients: Bacteriology Analysis
Prevalence of Peritonitis in Continuous Ambulatory Peritoneal Dialysis Patients: Bacteriology Analysis Open
View article: Multifocal osteolytic lesions as an initial presentation of mycobacterium riyadhense: Case report and literature review
Multifocal osteolytic lesions as an initial presentation of mycobacterium riyadhense: Case report and literature review Open
Mycobacterium riyadhense is a newly discovered, slow-growing nontuberculous mycobacterium with an emerging global distribution. We report a case of multifocal osteolytic lesions as the first sign of infection in a previously healthy 39-yea…
View article: Genomic surveillance of Methicillin Resistant <i>Staphylococcus aureus</i> in Saudi Arabia
Genomic surveillance of Methicillin Resistant <i>Staphylococcus aureus</i> in Saudi Arabia Open
Methicillin-resistant Staphylococcus aureus (MRSA) surveillance in regions with mass gatherings presents unique challenges for public health systems. Saudi Arabia, hosting millions of pilgrims annually, provides a distinctive setting for s…
View article: Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity
Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity Open
Similarity between candidate drug targets and human proteins is commonly assessed to minimize the occurrence of side effects. Although numerous drugs have been found to disrupt the health of the human microbiome, no comprehensive compariso…
View article: The emergence of highly resistant and hypervirulent Klebsiella pneumoniae CC14 clone in a tertiary hospital over 8 years
The emergence of highly resistant and hypervirulent Klebsiella pneumoniae CC14 clone in a tertiary hospital over 8 years Open
View article: Concurrent Clonal Expansion of Community-Associated Methicillin-resistant Staphylococcus aureus (MRSA) Clones in a Tertiary Hospital
Concurrent Clonal Expansion of Community-Associated Methicillin-resistant Staphylococcus aureus (MRSA) Clones in a Tertiary Hospital Open
Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat due to its ability to cause a range of diseases in humans and its resistance to multiple classes of antimicrobials. Community-associated MRSA (CA-M…
View article: Impact of the SARS-CoV-2 nucleocapsid 203K/204R mutations on the inflammatory immune response in COVID-19 severity
Impact of the SARS-CoV-2 nucleocapsid 203K/204R mutations on the inflammatory immune response in COVID-19 severity Open
Background The excessive inflammatory responses provoked by SARS-CoV-2 infection are critical factors affecting the severity and mortality of COVID-19. Previous work found that two adjacent co-occurring mutations R203K and G204R (KR) on th…
View article: Repurposing the oncolytic virus VSV∆51M as a COVID-19 vaccine
Repurposing the oncolytic virus VSV∆51M as a COVID-19 vaccine Open
The coronavirus disease 2019 (COVID-19) pandemic imposes an urgent and continued need for the development of safe and cost-effective vaccines to induce preventive responses for limiting major outbreaks around the world. To combat severe ac…
View article: Proteome profiling of nasopharynx reveals pathophysiological signature of COVID-19 disease severity
Proteome profiling of nasopharynx reveals pathophysiological signature of COVID-19 disease severity Open
Summary An aberrant innate immune system caused by the beta coronavirus SARS-CoV-2 is a characteristic manifestation of severe coronavirus disease 2019 (COVID-19). Here, we performed proteome profiling of nasopharyngeal (NP) swabs from 273…
View article: SARS-CoV-2 Omicron subvariant spike N405 unlikely to rapidly deamidate
SARS-CoV-2 Omicron subvariant spike N405 unlikely to rapidly deamidate Open
The RGD motif on the SARS-CoV-2 spike protein has been suggested to interact with RGD-binding integrins αVβ3 and α5β1 to enhance viral cell entry and alter downstream signaling cascades. The D405N mutation on the Omicron subvariant spike p…
View article: Effects of the nutrition of different diets and lipid content of the insect host larvae, Galleria mellonella on the efficacy of indigenous entomopathogenic nematodes
Effects of the nutrition of different diets and lipid content of the insect host larvae, Galleria mellonella on the efficacy of indigenous entomopathogenic nematodes Open
Entomopathogenic nematodes (EPNs) are promising as biocontrol agents for the most economically important insect pest attacking a wide range of host plants. Therefore, the aim of this work was to study the impact of four artificial diets an…
View article: The Emergence of Highly Resistant and Hypervirulent Klebsiella Pneumoniae CC14 Clone in a Tertiary Hospital Over Eight Years
The Emergence of Highly Resistant and Hypervirulent Klebsiella Pneumoniae CC14 Clone in a Tertiary Hospital Over Eight Years Open
View article: Additional file 2 of Impact of the SARS-CoV-2 nucleocapsid 203K/204R mutations on the inflammatory immune response in COVID-19 severity
Additional file 2 of Impact of the SARS-CoV-2 nucleocapsid 203K/204R mutations on the inflammatory immune response in COVID-19 severity Open
Additional file 2: Table S1. Demorgraphic and clinical information of all samples. Table S2. All significant DE genes (KR-patients versus Healthy). Table S3. All significant DE genes (RG-patients versus Healthy). Table S4. All DE genes (KR…
View article: SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load
SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load Open
View article: Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate Open
Middle East Respiratory Syndrome coronavirus (MERS-CoV) infects dromedary camels and zoonotically infects humans, causing a respiratory disease with severe pneumonia and death. With no approved antiviral or vaccine interventions for MERS, …
View article: Saudi Arabian SARS-CoV-2 genomes implicate a mutant Nucleocapsid protein in modulating host interactions and increased viral load in COVID-19 patients
Saudi Arabian SARS-CoV-2 genomes implicate a mutant Nucleocapsid protein in modulating host interactions and increased viral load in COVID-19 patients Open
Summary Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. The availability of patient hospital records is crucial for linking the genomic sequence information to virus func…
View article: Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses
Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses Open
Summary Viruses often encode proteins that mimic host proteins in order to facilitate infection. Little work has been done to understand the potential mimicry of the SARS-CoV-2, SARS-CoV, and MERS-CoV spike proteins, particularly the recep…
View article: Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate Open
Middle East Respiratory Syndrome coronavirus (MERS-CoV) infects dromedary camels and zoonotically infects humans, causing a respiratory disease with severe pneumonia and death. With no approved antiviral or vaccine interventions for MERS, …
View article: A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples (Global Challenges 4/2021)
A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples (Global Challenges 4/2021) Open
A critical bottleneck in COVID‐19 diagnosis and surveillance is the shortage of kits for RNA extraction. In article number 2000068, Mo Li and co‐workers develop a DIY, open‐source, safe, and fast method for magnetic‐nanoparticle‐aided vira…
View article: Simultaneous detection and mutation surveillance of SARS-CoV-2 and multiple respiratory viruses by rapid field-deployable sequencing
Simultaneous detection and mutation surveillance of SARS-CoV-2 and multiple respiratory viruses by rapid field-deployable sequencing Open
View article: Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes
Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes Open
One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one-step qRT-PCR is well suited for the diagnosis of COVID-19 and th…
View article: A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples
A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples Open
Molecular diagnosis and surveillance of pathogens such as SARS‐CoV‐2 depend on nucleic acid isolation. Pandemics at the scale of COVID‐19 can cause a global shortage of proprietary commercial reagents and BSL‐2 laboratories to safely perfo…
View article: Co-occurrence of mcr-1 and mcr-8 genes in multi-drug-resistant Klebsiella pneumoniae from a 2015 clinical isolate
Co-occurrence of mcr-1 and mcr-8 genes in multi-drug-resistant Klebsiella pneumoniae from a 2015 clinical isolate Open
View article: Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses
Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses Open
Viruses often encode proteins that mimic host proteins in order to facilitate infection. Little work has been done to understand the potential mimicry of the SARS-CoV-2, SARS-CoV, and MERS-CoV spike proteins, particularly the receptor-bind…
View article: Performance of Commercially Available Rapid Serological Assays for the Detection of SARS-CoV-2 Antibodies
Performance of Commercially Available Rapid Serological Assays for the Detection of SARS-CoV-2 Antibodies Open
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread globally. Although several rapid commercial serological assays have been developed, little is…