Shenwei Li
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View article: Treatment of trigeminal neuralgia by acupuncture combined with Chinese medicine from the perspective of modern medicine: A review
Treatment of trigeminal neuralgia by acupuncture combined with Chinese medicine from the perspective of modern medicine: A review Open
Trigeminal neuralgia (TN) is characterized by recurrent episodes of transient severe pain in its distribution area, with abrupt onset and termination. With the progression of the disease, patients are prone to concurrent psychiatric disord…
View article: Epidemiological and Genomic analysis of Vibrio parahaemolyticus isolated from imported travelers at the port of Shanghai, China (2017-2019)
Epidemiological and Genomic analysis of Vibrio parahaemolyticus isolated from imported travelers at the port of Shanghai, China (2017-2019) Open
Background Vibrio parahaemolyticus is the predominant etiological agent of seafood-associated foodborne illnesses on a global scale. It is essential to elucidate the mechanisms by which this pathogen disseminates. Given the existing resear…
View article: The efficacy of acupuncture combined with other therapies in post stroke cognitive impairment: A network meta-analysis
The efficacy of acupuncture combined with other therapies in post stroke cognitive impairment: A network meta-analysis Open
Background: The network meta-analysis was used to evaluate the efficacy of acupuncture combined with other therapies in the treatment of post stroke cognitive impairment (PSCI). Methods: The China National Knowledge Infrastructure, Wanfang…
View article: Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution Open
Despite the need in various applications, accurate quantification of nucleic acids still remains a challenge. The widely-used qPCR has reduced accuracy at ultralow template concentration and is susceptible to nonspecific amplifications. Th…
View article: Optimal scheduling strategy of the hydrogen-electric coupled system considering source-load uncertainty
Optimal scheduling strategy of the hydrogen-electric coupled system considering source-load uncertainty Open
Hybrid energy storage systems are increasingly envisaged to be used in the construction of microgrids to alleviate the intermittency of renewable energy output and achieve large-scale penetration into the power grid. The hydrogen-electric …
View article: RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern
RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern Open
Timely and accurate detection of SARS-CoV-2 variants of concern (VOCs) is urgently needed for pandemic surveillance and control. Great efforts have been made from a mass of scientists in increasing the detection sensitivity and operability…
View article: A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity
A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity Open
Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB)…
View article: Internet of medical things-enabled CRISPR diagnostics for rapid detection of SARS-CoV-2 variants of concern
Internet of medical things-enabled CRISPR diagnostics for rapid detection of SARS-CoV-2 variants of concern Open
Previous studies have highlighted CRISPR-based nucleic acid detection as rapid and sensitive diagnostic methods for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we reported an optimized CRISPR-Cas12a diagnostic platf…
View article: Increased genetic variation of A(H3N2) virus from influenza surveillance at the end of the 2016/2017 season for Shanghai port, China
Increased genetic variation of A(H3N2) virus from influenza surveillance at the end of the 2016/2017 season for Shanghai port, China Open
Influenza A(H3N2) virus exhibited complex seasonal patterns to evade pre-existing antibodies, resulting in changes in the antigenicity of the viron surface protein hemagglutinin (HA). To monitor the currently imported influenza viruses as …
View article: Research on safety and compliance of imported microbial inoculants using high-throughput sequencing
Research on safety and compliance of imported microbial inoculants using high-throughput sequencing Open
Microbial inoculants are widely used in wastewater treatment, soil remediation, and biological control. Safety and compliance for active constituents are considered to be the most important measures of imported microbial inoculants. Microb…
View article: RT-RPA-Cas12a-based discrimination of SARS-CoV-2 variants of concern
RT-RPA-Cas12a-based discrimination of SARS-CoV-2 variants of concern Open
Timely and accurate detection of SARS-CoV-2 variants of concern (VOCs) is urgently needed for pandemic surveillance and control. However, current methods are limited by the low sensitivity, long turn-around time or high cost. Here, we repo…
View article: Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe
Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe Open
Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressu…
View article: Programmed Exercise Attenuates Familial Hypertrophic Cardiomyopathy in Transgenic E22K Mice via Inhibition of PKC-α/NFAT Pathway
Programmed Exercise Attenuates Familial Hypertrophic Cardiomyopathy in Transgenic E22K Mice via Inhibition of PKC-α/NFAT Pathway Open
Familial hypertrophic cardiomyopathy (FHCM), an autosomal dominant disease, is caused by mutations in genes encoding cardiac sarcomeric proteins. E22K, a mutation in the myosin regulatory light chain sarcomere gene, is associated with the …
View article: Mitochondrial DNA Depletion Syndrome and Its Associated Cardiac Disease
Mitochondrial DNA Depletion Syndrome and Its Associated Cardiac Disease Open
Mitochondria is a ubiquitous, energy-supplying (ATP-based) organelle found in nearly all eukaryotes. It acts as a “power plant” by producing ATP through oxidative phosphorylation, providing energy for the cell. The bioenergetic functions o…
View article: RT-RPA-Cas12a-Based Discrimination of SARS-CoV-2 Variants of Concern
RT-RPA-Cas12a-Based Discrimination of SARS-CoV-2 Variants of Concern Open
View article: Complete genome sequence of GII.9 norovirus
Complete genome sequence of GII.9 norovirus Open
View article: Imported human norovirus in travelers, Shanghai port, China 2018: An epidemiological and whole genome sequencing study
Imported human norovirus in travelers, Shanghai port, China 2018: An epidemiological and whole genome sequencing study Open
Surveillance of the etiology and epidemiology of HuNoV global spread through travelers will improve pre-travel health advice, empirical treatment, and estimates of vaccine-preventable diseases.
View article: Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis
Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis Open
View article: A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses
A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses Open
View article: Comparative evaluation of 19 reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
Comparative evaluation of 19 reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2 Open
View article: Study on Mechanism of γ-PGA and Nattokinase Produced by Bacillus Subtilis Natto Based on RNA-Seq Analysis
Study on Mechanism of γ-PGA and Nattokinase Produced by Bacillus Subtilis Natto Based on RNA-Seq Analysis Open
Poly-γ-glutamic acid (γ-PGA) and nattokinase (NK) are the main substances produced by Bacillus subtilis natto in solid-state fermentation and have wide application prospects. We found that our strains have higher nattokinase activity when …
View article: Comparative Evaluation of 19 Reverse Transcription Loop-Mediated Isothermal Amplification Assays for Detection of SARS-CoV-2
Comparative Evaluation of 19 Reverse Transcription Loop-Mediated Isothermal Amplification Assays for Detection of SARS-CoV-2 Open
Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has caused a global pandemics. To facilitate the detection of SARS-CoV-2 infection, various RT-LAMP assays using 19 sets of primers had been developed, but never been compared. We pe…
View article: A Proposal for the Classification and Nomenclature of Hand, Foot and Mouth Disease-Related Enteroviruses
A Proposal for the Classification and Nomenclature of Hand, Foot and Mouth Disease-Related Enteroviruses Open
Background: Enterovirus has diverged into many types, some of which cause hand, foot and mouth disease (HFMD) in children. The predominant enterovirus types associated with HFMD are EVA71, CVA16, CVA6 and CVA10. Subtyping of these enterovi…
View article: The Phylodynamics of Seasonal Influenza A/H1N1pdm Virus in China Between 2009 and 2019
The Phylodynamics of Seasonal Influenza A/H1N1pdm Virus in China Between 2009 and 2019 Open
Since its first introduction into China in 2009, influenza A/H1N1pdm virus has undergone a rapid expansion and replaced the classical seasonal A(H1N1) virus. To characterize the ongoing evolution and national transmission dynamics of this …