Shinichi Hochi
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View article: Erratum: Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa
Erratum: Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa Open
J Reprod Dev, Vol. 70, No. 4, p. 255 Table 2 have been corrected. For the bottom of Survived (%) row, the Survived value, which read "69", should be replaced with "86".
View article: Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa
Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa Open
Intracytoplasmic sperm injection (ICSI) is clinically used to treat obstructive/nonobstructive azoospermia. This study compared the efficacy of ICSI with cauda epididymal and testicular sperm in Wistar (WI) and Brown-Norway (BN) rats. The …
View article: Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos
Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos Open
Y-box binding protein 1 ( YBX1 ) plays important roles in RNA stabilization, translation, transcriptional regulation, and mitophagy. However, its effects on porcine preimplantation embryos remain unclear. In this study, we knocked down YBX…
View article: Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells Open
View article: Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10.
Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10. Open
Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More …
View article: High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection
High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection Open
Intracytoplasmic sperm injection (ICSI) is an alternative technique to in vitro fertilization (IVF) for producing transferable blastocysts, especially in combination with cryopreserved oocytes, when the IVF system does not work sufficientl…
View article: Cryodevices developed for minimum volume cooling vitrification of bovine oocytes
Cryodevices developed for minimum volume cooling vitrification of bovine oocytes Open
Unfertilized bovine oocytes can be efficiently cryopreserved only when an extremely rapid cooling rate (>20,000°C/min) is applied to oocytes with a very limited amount of surrounding vitrification solution. This protocol is defined as mini…
View article: Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats Open
View article: Transplantation of rat pancreatic islets vitrified-warmed on the nylon mesh device and the silk fibroin sponge disc
Transplantation of rat pancreatic islets vitrified-warmed on the nylon mesh device and the silk fibroin sponge disc Open
We report the adaptability of rat islets vitrified-warmed on nylon mesh (NM) device or silk fibroin (SF) sponge disc for the normalization of the blood glucose level in rat models of diabetes. One-hundred rat islets were cryopreserved acco…
View article: Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats
Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats Open
View article: FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche
FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche Open
Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this stud…
View article: Haploid embryonic stem cell lines derived from androgenetic and parthenogenetic rat blastocysts
Haploid embryonic stem cell lines derived from androgenetic and parthenogenetic rat blastocysts Open
The present study was conducted to establish haploid embryonic stem (ES) cell lines using fluorescent marker-carrying rats. In the first series, 7 ES cell lines were established from 26 androgenetic haploid blastocysts. However, only 1 ES …
View article: Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies
Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies Open
Centrosome integrity and microtubule network are crucial to the events around fertilization, including pronuclear development, migration and fusion, and the first mitotic division. The present review highlights the importance of bull sperm…
View article: High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol
High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol Open
The objective of this study was to investigate whether developmental competence of vitrified–warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l -as…