Explanipedia

Integration of signals inducing reproductive phase transition occurs at the nodal enlarged vascular bundles in sacred lotus, Nelumbo nucifera Open

Ryusei Waizumi, Shizuko Ichinose, Misaki Inoue, Motoyuki Ishimori, Michio Shibata , et al. · 2025

Sacred lotus (Nelumbo nucifera) is a perennial aquatic plant classified into basal eudicots, propagating through both sexual (flowering) and vegetative (clonal) reproduction. In lotus, the transition from sexual to clonal reproductive phas…

Vascular Basement Membrane Fragmentation in Keloids and the Expression of Key Basement Membrane Component Genes Open

Yusaku Saijo, Shizuko Ichinose, Teruyuki Dohi, Rei Ogawa · 2024

Background: Keloids are growing scars that arise from injury to the reticular dermis and subsequent chronic local inflammation. The latter may be promoted by vascular hyperpermeability, which permits the ingress of chronic inflammatory cel…

Treatment with YIGSR peptide ameliorates mouse tail lymphedema by 67 kDa laminin receptor (67LR)-dependent cell-cell adhesion Open

Yuta Sakae, H. Takada, Shizuko Ichinose, Masaaki Nakajima, Atsushi Sakai , et al. · 2023

Impaired microcirculation can cause lymphatic leakage which leads to a chronic swelling in the tissues of the body. However, no successful treatment gives any protection against lymphedema due to the lack of well-revealed pathophysiology o…

Sphingomyelin Is Essential for the Structure and Function of the Double-Membrane Vesicles in Hepatitis C Virus RNA Replication Factories Open

Hossam Gewaid, Haruyo Aoyagi, Minetaro Arita, Koichi Watashi, Ryosuke Suzuki , et al. · 2020

Previous reports assumed that sphingomyelin (SM) is essential for HCV replication, but the mechanism was unclear. In this study, we showed for the first time that SM and ceramide transfer protein (CERT), which is in the SM biosynthesis pat…

Noncontact Phased-Array Ultrasound Facilitates Acute Wound Healing in Mice Open

Nao Wakabayashi, Atsushi Sakai, Hiroya Takada, Takayuki Hoshi, Hitomi Sano , et al. · 2019

Background: The authors developed a noncontact low-frequency ultrasound device that delivers high-intensity mechanical force based on phased-array technology. It may aid wound healing because it is likely to be associated with lower risks …

PB-15 Morphological Analysis of Reticular Dermis of Human Keloid Tissue Open

Shizuko Ichinose, Chiemi Kaku, Rei Ogawa · 2019

KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections Open

Miki Hara‐Yokoyama, Hidetake Kurihara, Shozo Ichinose, Hironori Matsuda, Shizuko Ichinose , et al. · 2019

The arrangement of immature germ cells changes regularly and periodically along the axis of the seminiferous tubule, and is used to describe the progression of spermatogenesis. This description is based primarily on the changes in the acro…

An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia Open

Toshiro Saito, Jihoon Nah, Shinichi Oka, Risa Mukai, Yoshiya Monden , et al. · 2018

Energy stress, such as ischemia, induces mitochondrial damage and death in the heart. Degradation of damaged mitochondria by mitophagy is essential for the maintenance of healthy mitochondria and survival. Here, we show that mitophagy duri…

Petaloid recombinant peptide enhances in vitro cartilage formation by synovial mesenchymal stem cells Open

Mana Naritomi, Mitsuru Mizuno, Hisako Katano, Nobutake Ozeki, Koji Otabe , et al. · 2018

In vitro chondrogenesis of mesenchymal stem cells (MSCs) mimics in vivo chondrogenesis of MSCs. However, the size of the cartilage pellets that can be attained in vitro is limited by current methods; therefore, some modifications are requi…

Specific markers and properties of synovial mesenchymal stem cells in the surface, stromal, and perivascular regions Open

Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose , et al. · 2018

We identified specific markers to isolate synovial cells from the surface, stromal, and perivascular regions of the synovium. Synovial MSCs in the perivascular region had the highest proliferative and chondrogenic potentials among the thre…

Intrinsic Autophagy Is Required for the Maintenance of Intestinal Stem Cells and for Irradiation-Induced Intestinal Regeneration Open

Jumpei Asano, Taku Sato, Shizuko Ichinose, Mihoko Kajita, Nobuyuki Onai , et al. · 2017

Autophagy is a lysosomal degradation pathway with important roles in physiological homeostasis and disease. However, the role of autophagy in intestinal stem cells (ISCs) is unclear. Here, we show that intrinsic autophagy in ISCs is import…

The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system Open

Ayaka Ohashi, Aya Murata, Yuichiro Cho, Shizuko Ichinose, Yuriko Sakamaki , et al. · 2017

RNA may be released from vascular cells including endothelial cells in the event of injury and in vascular disease. Extracellular RNAs have been recognized as novel procoagulant and permeability-increasing factors. Extracellular RNA may fu…

A novel form of necrosis, TRIAD, occurs in human Huntington’s disease Open

Emiko Yamanishi, Kazuko Hasegawa, Kyota Fujita, Shizuko Ichinose, Saburo Yagishita , et al. · 2017

Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs Open

Ryo Nakamichi, Yoshiaki Ito, Masafumi Inui, Naoko Onizuka, Tomohiro Kayama , et al. · 2016

The main pathogenesis of intervertebral disc (IVD) herniation involves disruption of the annulus fibrosus (AF) caused by ageing or excessive mechanical stress and the resulting prolapse of the nucleus pulposus. Owing to the avascular natur…

Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossification of Achilles tendon via failed tenogenesis Open

Hidetsugu Suzuki, Yoshiaki Ito, Masahiro Shinohara, Satoshi Yamashita, Shizuko Ichinose , et al. · 2016

Significance Molecular mechanisms of tendon development and homeostasis are not well understood. Generation and analysis of Mkx −/− rats revealed new functions of Mohawk (Mkx) in mediating cellular responses to mechanical stress. An Mkx-Ch…

Histological analysis of the superficial zone in regenerated cartilage. Open

Yusuke Nakagawa, Muneta Takeshi, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno , et al. · 2016

(A) Sagittal sections stained with Safranin-O/Fast Green. (B) Sagittal sections unstained for polarizing microscope. (C) TEM images for the superficial zone.

Regenerated cartilage immunostained with GFP and lubricin. Open

Yusuke Nakagawa, Muneta Takeshi, Koji Otabe, Nobutake Ozeki, Mizuno Mitsuru , et al. · 2016

(A) Sagittal sections immunostained with GFP. (B) Histological observation under fluorescence for GFP, Hoechst 33342, and lubricin. White arrows indicate both lubricin and GFP positive cells in the superficial zone.

Preparation, appearance and gene expressions of aggregates of human BM MSCs after hanging drop culture. Open

Nakagawa Yusuke, Muneta Takeshi, Koji Otabe, Nobutake Ozeki, Mizuno Mitsuru , et al. · 2016

(A) Schema for the study. 2.5 × 10⁵ human BM MSCs were cultured for 3 days in hanging drops. (B) Drops hanging on the cover of a 15 cm dish. (C) Macroscopic images of aggregates consisting of 2.5×10⁵ MSCs at 10 minute…

Lubricin expression in human BM MSCs after pellet culture. Open

Nakagawa Yusuke, Muneta Takeshi, Koji Otabe, Nobutake Ozeki, Mizuno Mitsuru , et al. · 2016

(A) Schema for the study of in vitro chondrogenesis. BM MSCs were prepared from 5 donors. 2.5 × 10⁵ human BM MSCs at passage 2 were pelleted and cultured in chondrogenic medium for 7 or 21 days. (B) Histological sections …

Cartilage regeneration by transplantation of aggregates of BM MSCs in rats. Open

Yusuke Nakagawa, Muneta Takeshi, Koji Otabe, Nobutake Ozeki, Mizuno Mitsuru , et al. · 2016

(A) Schema for the study. BM MSCs were obtained from GFP transgenic rats. Three days before the surgery, passage 2 GFP positive MSCs were aggregated by hanging drop culture. Osteochondral defect was created in the knee joint. For the MSC g…

Gtf2ird1-Dependent Mohawk Expression Regulates Mechanosensing Properties of the Tendon Open

Tomohiro Kayama, Masaki Mori, Yoshiaki Ito, Takahide Matsushima, Ryo Nakamichi , et al. · 2016

Mechanoforces experienced by an organ are translated into biological information for cellular sensing and response. In mammals, the tendon connective tissue experiences and resists physical forces, with tendon-specific mesenchymal cells ca…

Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo Open

Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno , et al. · 2016

Cartilage derived from MSCs expressed lubricin protein both in vitro and in vivo. Aggregation promoted lubricin expression of MSCs in vitro and transplantation of aggregates of MSCs regenerated cartilage including the superficial zone in a…

Prolactin Regulatory Element Binding Protein Is Involved in Hepatitis C Virus Replication by Interaction with NS4B Open

Lingbao Kong, Akira Fujimoto, Mariko Nakamura, Haruyo Aoyagi, Mami Matsuda , et al. · 2016

It has been proposed that the hepatitis C virus (HCV) NS4B protein triggers the membranous HCV replication compartment, but the underlying molecular mechanism is not fully understood. Here, we screened for NS4B-associated membrane proteins…

Energy output reduction and surface alteration of quartz tips following Er:YAG laser contact irradiation on soft and hard tissues in vitro Open

Taichen Lin, Rie Kawamura, Akira Aoki, Shizuko Ichinose, Koji Mizutani , et al. · 2016

Though the Er:YAG laser (ErL) has been used in periodontal therapy, the irradiated tip damage has not been studied in detail. In this study, the change in the energy output, surface morphology, and temperature of quartz tips was evaluated …

Mohawk transcription factor regulates homeostasis of the periodontal ligament Open

Naoki Koda, Tempei Sato, Masahiro Shinohara, Shizuko Ichinose, Yoshiaki Ito , et al. · 2016

The periodontal ligament (PDL), which connects the teeth to the alveolar bone, is essential for periodontal tissue homeostasis. Although the significance of the PDL is recognized, molecular mechanisms underlying PDL function are not well-k…

Pre-embedding immunoelectron microscopic analysis shows the presence of Mieap and lysosomal proteins within mitochondria. Open

Yuji Miyamoto, Noriaki Kitamura, Yasuyuki Nakamura, Manabu Futamura, Takafumi Miyamoto , et al. · 2015

The Ad-Mieap and Ad-LacZ infected cells of HCT116 were subjected to pre-embedding imunoelectron microscopic analysis with DAB on day 3 after IR. The primary antibody against Mieap (A), cathepsin D (B), or LAMP1 (C) was used for the analysi…

Relationship between MRI T1rho value and histological findings of intact and radially incised menisci in microminipigs Open

Yusuke Nakagawa, Ichiro Sekiya, Shimpei Kondo, Takashi Tabuchi, Shizuko Ichinose , et al. · 2015

Purpose To examine whether the T 1 rho value reflects histological changes in menisci we analyzed the relationship between T 1 rho value and histological findings in intact and radially incised menisci of pigs. Materials and Methods Seven …

Foxc1 is required for early stage telencephalic vascular development Open

Thanit Prasitsak, Nandar Mya, Shigeru Okuhara, Shizuko Ichinose, Masato S. Ota , et al. · 2015

Background : The brain vascular system arises from the perineural vascular plexus (PNVP) which sprouts radially into the neuroepithelium and subsequently branches off laterally to form a secondary plexus in the subventricular zone (SVZ), t…

Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs Open

Yusuke Nakagawa, Takeshi Muneta, Shimpei Kondo, Mitsuru Mizuno, Kazuo Takakuda , et al. · 2015

TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis Open

Yohei Matsubara, Tomoko Kato, Kenichi Kashimada, Hiromitsu Tanaka, Zhi Zhou , et al. · 2015

The Y chromosome plays a critical role in spermatogenesis. Formerly, it had been difficult to generate knockout mice with specific Y chromosome mutations using conventional gene-targeting strategies. Recently, a transcription activator-lik…

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