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View article: Dysplastic lung repair fosters a tuberculosis-promoting microenvironment through maladaptive macrophage polarization
Dysplastic lung repair fosters a tuberculosis-promoting microenvironment through maladaptive macrophage polarization Open
Pulmonary TB that develops in immunocompetent adult humans is responsible for approximately 85% of the disease burden and is central for Mtb transmission. Most humans contain Mtb infection within primary granulomatous lesions, but in certa…
View article: Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates <i>Mycobacterium tuberculosis</i> sensitivity to nitric oxide stress
Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates <i>Mycobacterium tuberculosis</i> sensitivity to nitric oxide stress Open
Successful host colonization by bacterial pathogens requires appropriate response and adaptation to environmental signals encountered during infection, with two-component systems (TCSs) and serine/threonine protein kinases (STPKs) being tw…
View article: Direct visualization of bacterial transcripts in the infected lung illuminates spatiotemporal environmental adaptation of <i>Mycobacterium tuberculosis</i>
Direct visualization of bacterial transcripts in the infected lung illuminates spatiotemporal environmental adaptation of <i>Mycobacterium tuberculosis</i> Open
SUMMARY Spatiotemporal environmental variation results in marked heterogeneity in bacterial infection progression and disease outcome, with vital consequences for treatment success. For the globally important pathogen Mycobacterium tubercu…
View article: Increased proportion of growth-arrested bacilli in acidic pH adaptation promotes <i>Mycobacterium tuberculosis</i> treatment survival
Increased proportion of growth-arrested bacilli in acidic pH adaptation promotes <i>Mycobacterium tuberculosis</i> treatment survival Open
The ability of Mycobacterium tuberculosis (Mtb) to adapt its growth behavior in response to host environments promotes survival against immune and drug stressors. However, how these behaviors shift at the single-cell level remains poorly u…
View article: Differentiating <i>Peromyscus leucopus</i> bone marrow-derived macrophages for characterization of responses to <i>Borrelia burgdorferi</i> and lipopolysaccharide
Differentiating <i>Peromyscus leucopus</i> bone marrow-derived macrophages for characterization of responses to <i>Borrelia burgdorferi</i> and lipopolysaccharide Open
Currently, most tools utilized in host-pathogen interaction studies depend on the use of human or mouse ( Mus musculus ) cells and tissues. While these species have led to countless breakthroughs in our understanding of infectious disease,…
View article: Cholesterol metabolism and intrabacterial potassium homeostasis are intrinsically related in Mycobacterium tuberculosis
Cholesterol metabolism and intrabacterial potassium homeostasis are intrinsically related in Mycobacterium tuberculosis Open
Potassium (K + ) is the most abundant intracellular cation, but much remains unknown regarding how K + homeostasis is integrated with other key bacterial biology aspects. Here, we show that K + homeostasis disruption (CeoBC K + uptake syst…
View article: Weakened Airway Epithelial Junctions and Enhanced Neutrophil Elastase Release Contribute to Age‐Dependent Bacteremia Risk Following Pneumococcal Pneumonia
Weakened Airway Epithelial Junctions and Enhanced Neutrophil Elastase Release Contribute to Age‐Dependent Bacteremia Risk Following Pneumococcal Pneumonia Open
Streptococcus pneumoniae ( Sp ; pneumococcus), the most common agent of community‐acquired pneumonia, can spread systemically, particularly in the elderly, highlighting the need for adjunctive therapies. The airway epithelial barrier defen…
View article: Differentiating <i>Peromyscus leucopus</i> bone marrow-derived macrophages for characterization of responses to <i>Borrelia burgdorferi</i> and lipopolysaccharide
Differentiating <i>Peromyscus leucopus</i> bone marrow-derived macrophages for characterization of responses to <i>Borrelia burgdorferi</i> and lipopolysaccharide Open
Currently, most tools utilized in host-pathogen interaction studies depend on the use of human or mouse ( Mus musculus ) cells and tissues. While these species have led to countless breakthroughs in our understanding of infectious disease,…
View article: Cholesterol metabolism and intrabacterial potassium homeostasis are intrinsically related in<i>Mycobacterium tuberculosis</i>
Cholesterol metabolism and intrabacterial potassium homeostasis are intrinsically related in<i>Mycobacterium tuberculosis</i> Open
SUMMARY Potassium (K + ) is the most abundant intracellular cation, but much remains unknown regarding how K + homeostasis is integrated with other key bacterial biology aspects. Here, we show that K + homeostasis disruption (CeoBC K + upt…
View article: Noisy Dueling Double Deep Q-Network algorithm for autonomous underwater vehicle path planning
Noisy Dueling Double Deep Q-Network algorithm for autonomous underwater vehicle path planning Open
How to improve the success rate of autonomous underwater vehicle (AUV) path planning and reduce travel time as much as possible is a very challenging and crucial problem in the practical applications of AUV in the complex ocean current env…
View article: Weakened airway epithelial junctions and enhanced neutrophil elastase release contribute to age-dependent bacteremia risk following pneumococcal pneumonia
Weakened airway epithelial junctions and enhanced neutrophil elastase release contribute to age-dependent bacteremia risk following pneumococcal pneumonia Open
Streptococcus pneumoniae ( Sp ; pneumococcus), the most common agent of community-acquired pneumonia, can spread systemically, particularly in the elderly, highlighting the need for adjunctive therapies. The airway epithelial barrier defen…
View article: Blocking HXA <sub>3</sub> -mediated neutrophil elastase release during <i>S. pneumoniae</i> lung infection limits pulmonary epithelial barrier disruption and bacteremia
Blocking HXA <sub>3</sub> -mediated neutrophil elastase release during <i>S. pneumoniae</i> lung infection limits pulmonary epithelial barrier disruption and bacteremia Open
Streptococcus pneumoniae ( Sp ), a leading cause of community-acquired pneumonia, can spread from the lung into the bloodstream to cause septicemia and meningitis, with a concomitant threefold increase in mortality. Limitations in vaccine …
View article: Blocking HXA<sub>3</sub>-mediated neutrophil elastase release during<i>S. pneumoniae</i>lung infection limits pulmonary epithelial barrier disruption and bacteremia
Blocking HXA<sub>3</sub>-mediated neutrophil elastase release during<i>S. pneumoniae</i>lung infection limits pulmonary epithelial barrier disruption and bacteremia Open
Streptococcus pneumoniae ( Sp ), a leading cause of community-acquired pneumonia, can spread from the lung into the bloodstream to cause septicemia and meningitis, with a concomitant three-fold increase in mortality. Limitations in vaccine…
View article: Mycobacterium tuberculosis response to cholesterol is integrated with environmental pH and potassium levels via a lipid metabolism regulator
Mycobacterium tuberculosis response to cholesterol is integrated with environmental pH and potassium levels via a lipid metabolism regulator Open
Successful colonization of the host requires Mycobacterium tuberculosis (Mtb) to sense and respond coordinately to disparate environmental cues during infection and adapt its physiology. However, how Mtb response to environmental cues and …
View article: Aberrant macrophage activation and maladaptive lung repair promote tuberculosis progression uniquely in the lung
Aberrant macrophage activation and maladaptive lung repair promote tuberculosis progression uniquely in the lung Open
Pulmonary tuberculosis (PTB) represents 85% of the disease burden caused by Mycobacterium tuberculosis (Mtb) and promotes aerosol transmission infecting about a quarter of people globally. Most Mtb infections are effectively limited within…
View article: <i>Mycobacterium tuberculosis</i>response to cholesterol is integrated with environmental pH and potassium levels via a lipid utilization regulator
<i>Mycobacterium tuberculosis</i>response to cholesterol is integrated with environmental pH and potassium levels via a lipid utilization regulator Open
SUMMARY How bacterial response to environmental cues and nutritional sources may be integrated in enabling host colonization is poorly understood. Exploiting a reporter-based screen, we discovered that overexpression of Mycobacterium tuber…
View article: Pore-forming activity of <i>S. pneumoniae</i> pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin
Pore-forming activity of <i>S. pneumoniae</i> pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin Open
Streptococcus pneumoniae, a common cause of community-acquired bacterial pneumonia, can cross the respiratory epithelial barrier to cause lethal septicemia and meningitis. S. pneumoniae pore-forming toxin pneumolysin (PLY) triggers robust …
View article: Pore-forming activity of<i>S. pneumoniae</i>pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin
Pore-forming activity of<i>S. pneumoniae</i>pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin Open
Streptococcus pneumoniae, a common cause of community-acquired bacterial pneumonia, can cross the respiratory epithelial barrier to cause lethal septicemia and meningitis. S. pneumoniae pore-forming toxin pneumolysin (PLY) triggers robust …
View article: Shining a light on bacterial environmental cue integration and its relation to metabolism
Shining a light on bacterial environmental cue integration and its relation to metabolism Open
The ability of a bacterium to successfully colonize its host is dependent on proper adaptation to its local environment. Environmental cues are diverse in nature, ranging from ions to bacterial‐produced signals, and to host immune response…
View article: Liquid chromatography-mass spectrometry-based protocol to measure drug accumulation in Mycobacterium tuberculosis and its host cell
Liquid chromatography-mass spectrometry-based protocol to measure drug accumulation in Mycobacterium tuberculosis and its host cell Open
The extent to which a drug accumulates in Mycobacterium tuberculosis (Mtb) and its host cell can affect treatment efficacy. We describe protocols measuring drug accumulation in Mtb, macrophages, and Mtb-infected macrophages. The method lev…
View article: Interbank complex network and liquidity creation: Evidence from European banks
Interbank complex network and liquidity creation: Evidence from European banks Open
Liquidity creation, as a core functions of banks, affects the stability of the financial system and economic development significantly. However, the existing literature has largely ignored the impact of complex interbank linkages on liquid…
View article: An <scp>AP</scp>‐3‐dependent pathway directs phagosome fusion with Rab8 and Rab11 vesicles involved in <scp>TLR2</scp> signaling
An <span>AP</span>‐3‐dependent pathway directs phagosome fusion with Rab8 and Rab11 vesicles involved in <span>TLR2</span> signaling Open
Intracellular compartmentalization of ligands, receptors and signaling molecules has been recognized as an important regulator of inflammation. The toll‐like receptor (TLR) 2 pathway utilizes the trafficking molecule adaptor protein 3 (AP‐…
View article: Analysis of the association between <scp>MICA</scp> gene polymorphisms and schizophrenia
Analysis of the association between <span>MICA</span> gene polymorphisms and schizophrenia Open
Background The major histocompatibility complex (MHC) has been implicated in schizophrenia. This study aimed to explore the correlation between the major histocompatibility complex class I polypeptide‐related sequence A (MICA) polymorphism…
View article: PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine/threonine protein kinases
PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine/threonine protein kinases Open
The ability of Mycobacterium tuberculosis (Mtb) to adapt to its surrounding environment is critical for the bacterium to successfully colonize its host. Transcriptional changes are a vital mechanism by which Mtb responds to key environment…
View article: Spatial relationships of intra-lesion heterogeneity in Mycobacterium tuberculosis microenvironment, replication status, and drug efficacy
Spatial relationships of intra-lesion heterogeneity in Mycobacterium tuberculosis microenvironment, replication status, and drug efficacy Open
A hallmark of Mycobacterium tuberculosis (Mtb) infection is the marked heterogeneity that exists, spanning lesion type differences to microenvironment changes as infection progresses. A mechanistic understanding of how this heterogeneity a…
View article: PrrA modulates <i>Mycobacterium tuberculosis</i> response to multiple environmental cues and is critically regulated by serine/threonine protein kinases
PrrA modulates <i>Mycobacterium tuberculosis</i> response to multiple environmental cues and is critically regulated by serine/threonine protein kinases Open
The ability of Mycobacterium tuberculosis (Mtb) to adapt to its surrounding environment is critical for the bacterium to successfully colonize its host. Transcriptional changes are a vital mechanism by which Mtb responds to key environment…
View article: <scp>Rv0500A</scp> is a transcription factor that links <i>Mycobacterium tuberculosis</i> environmental response with division and impacts host colonization
<span>Rv0500A</span> is a transcription factor that links <i>Mycobacterium tuberculosis</i> environmental response with division and impacts host colonization Open
For Mycobacterium tuberculosis (Mtb) to successfully infect a host, it must be able to adapt to changes in its microenvironment, including variations in ionic signals such as pH and chloride (Cl − ), and link these responses to its growth.…
View article: Spatial relationships of intra-lesion heterogeneity in <i>Mycobacterium tuberculosis</i> microenvironment, replication status, and drug efficacy
Spatial relationships of intra-lesion heterogeneity in <i>Mycobacterium tuberculosis</i> microenvironment, replication status, and drug efficacy Open
A hallmark of Mycobacterium tuberculosis (Mtb) infection with critical impact on disease development and outcome is the marked heterogeneity that exists, spanning differences in lesion types to changes in microenvironment as the infection …
View article: <i>Yersinia pseudotuberculosis</i> YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers
<i>Yersinia pseudotuberculosis</i> YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers Open
Many pathogens use M cells to access the underlying Peyer’s patches and spread to systemic sites via the lymph as demonstrated by ligated loop murine intestinal models. However, the study of interactions between M cells and microbial patho…