Shuishu Wang
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View article: Vaccine Elicitation of HIV-1 Neutralizing Antibodies Against Both V2 Apex and Fusion Peptide in Rhesus Macaques
Vaccine Elicitation of HIV-1 Neutralizing Antibodies Against Both V2 Apex and Fusion Peptide in Rhesus Macaques Open
SUMMARY Broadly neutralizing antibodies targeting multiple sites of HIV-1 Env vulnerability can be induced by infection, but simultaneous elicitation of neutralizing antibodies (NAbs) against multiple epitopes has not yet been achieved by …
View article: The N terminus of H3-influenza hemagglutinin as a site-of-vulnerability to neutralizing antibody
The N terminus of H3-influenza hemagglutinin as a site-of-vulnerability to neutralizing antibody Open
The N terminus of the H3 subtype of influenza virus hemagglutinin is ∼10 residues longer than the N termini of most other hemagglutinins. As conserved, exposed, and linear regions may be good vaccine targets, we investigated the vaccine ut…
View article: Weak Supervision Techniques towards Enhanced ASR Models in Industry-level CRM Systems
Weak Supervision Techniques towards Enhanced ASR Models in Industry-level CRM Systems Open
In the design of customer relationship management (CRM) systems, accurately identifying customer types and offering personalized services are key to enhancing customer satisfaction and loyalty. However, this process faces the challenge of …
View article: The N Terminus of H3-Influenza Hemagglutinin as a Site-of-Vulnerability to Neutralizing Antibody
The N Terminus of H3-Influenza Hemagglutinin as a Site-of-Vulnerability to Neutralizing Antibody Open
View article: Design, Synthesis, and Bio-Evaluation of C2-Aryl Galantamine Derivatives
Design, Synthesis, and Bio-Evaluation of C2-Aryl Galantamine Derivatives Open
View article: Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide
Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide Open
Broadly neutralizing antibodies have been proposed as templates for HIV-1 vaccine design, but it has been unclear how similar vaccine-elicited antibodies are to their naturally elicited templates. To provide insight, here we compare the re…
View article: Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide
Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide Open
Broadly neutralizing antibodies have been proposed as templates for HIV-vaccine design, but it has been unclear how similar vaccine-elicited antibodies are to their naturally elicited templates. To provide insight, here we compare the reco…
View article: Cholesterol reduction by immunization with a PCSK9 mimic
Cholesterol reduction by immunization with a PCSK9 mimic Open
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a plasma protein that controls cholesterol homeostasis. Here, we design a human PCSK9 mimic, named HIT01, with no consecutive 9-residue stretch in common with any human protein as a …
View article: Anti-idiotype isolation of a broad and potent influenza A virus-neutralizing human antibody
Anti-idiotype isolation of a broad and potent influenza A virus-neutralizing human antibody Open
The VH6-1 class of antibodies includes some of the broadest and most potent antibodies that neutralize influenza A virus. Here, we elicit and isolate anti-idiotype antibodies against germline versions of VH6-1 antibodies, use these to sort…
View article: Ultrapotent Broadly Neutralizing Human‐llama Bispecific Antibodies against HIV‐1
Ultrapotent Broadly Neutralizing Human‐llama Bispecific Antibodies against HIV‐1 Open
Broadly neutralizing antibodies are proposed as therapeutic and prophylactic agents against HIV‐1, but their potency and breadth are less than optimal. This study describes the immunization of a llama with the prefusion‐stabilized HIV‐1 en…
View article: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base
Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base Open
Soluble 'SOSIP'-stabilized HIV-1 envelope glycoprotein (Env) trimers elicit dominant antibody responses targeting their glycan-free base regions, potentially diminishing neutralizing responses. Previously, using a nonhuman primate model, w…
View article: Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability
Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability Open
Lassa virus (LASV) infection is expanding outside its traditionally endemic areas in West Africa, posing a pandemic biothreat. LASV-neutralizing antibodies, moreover, have proven difficult to elicit. To gain insight into LASV neutralizatio…
View article: Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5
Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5 Open
The receptor-binding domain (RBD) of the SARS-CoV-2 spike is a primary target of neutralizing antibodies and a key component of licensed vaccines. Substantial mutations in RBD, however, enable current variants to escape immunogenicity gene…
View article: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies Open
View article: Structure-based design of a single-chain triple-disulfide-stabilized fusion-glycoprotein trimer that elicits high-titer neutralizing responses against human metapneumovirus
Structure-based design of a single-chain triple-disulfide-stabilized fusion-glycoprotein trimer that elicits high-titer neutralizing responses against human metapneumovirus Open
The Pneumoviridae family of viruses includes human metapneumovirus (HMPV) and respiratory syncytial virus (RSV). The closely related Paramyxoviridae family includes parainfluenza viruses (PIVs). These three viral pathogens cause acute resp…
View article: Enhancing Anti-SARS-CoV-2 Neutralizing Immunity by Genetic Delivery of Enveloped Virus-like Particles Displaying SARS-CoV-2 Spikes
Enhancing Anti-SARS-CoV-2 Neutralizing Immunity by Genetic Delivery of Enveloped Virus-like Particles Displaying SARS-CoV-2 Spikes Open
New vaccine delivery technologies, such as mRNA, have played a critical role in the rapid and efficient control of SARS-CoV-2, helping to end the COVID-19 pandemic. Enveloped virus-like particles (eVLPs) are often more immunogenic than pro…
View article: Soluble prefusion-closed HIV-envelope trimers with glycan-covered bases
Soluble prefusion-closed HIV-envelope trimers with glycan-covered bases Open
View article: HIV-1 neutralizing antibodies elicited in humans by a prefusion-stabilized envelope trimer form a reproducible class targeting fusion peptide
HIV-1 neutralizing antibodies elicited in humans by a prefusion-stabilized envelope trimer form a reproducible class targeting fusion peptide Open
Elicitation of antibodies that neutralize the tier-2 neutralization-resistant isolates that typify HIV-1 transmission has been a long-sought goal. Success with prefusion-stabilized envelope trimers eliciting autologous neutralizing antibod…
View article: Diverse Murine Vaccinations Reveal Distinct Antibody Classes to Target Fusion Peptide and Variation in Peptide Length to Improve HIV Neutralization
Diverse Murine Vaccinations Reveal Distinct Antibody Classes to Target Fusion Peptide and Variation in Peptide Length to Improve HIV Neutralization Open
The HIV-1 fusion peptide has been identified as a site for elicitation of broadly neutralizing antibodies, with prior studies demonstrating that priming with fusion peptide-based immunogens and boosting with soluble envelope (Env) trimers …
View article: Bispecific antibody CAP256.J3LS targets V2-apex and CD4-binding sites with high breadth and potency
Bispecific antibody CAP256.J3LS targets V2-apex and CD4-binding sites with high breadth and potency Open
Antibody CAP256-VRC26.25 targets the second hypervariable region (V2) at the apex of the HIV envelope (Env) trimer with extraordinary neutralization potency, although less than optimal breadth. To improve breadth, we linked the light chain…
View article: Assessment of Crosslinkers between Peptide Antigen and Carrier Protein for Fusion Peptide-Directed Vaccines against HIV-1
Assessment of Crosslinkers between Peptide Antigen and Carrier Protein for Fusion Peptide-Directed Vaccines against HIV-1 Open
Conjugate-vaccine immunogens require three components: a carrier protein, an antigen, and a crosslinker, capable of coupling antigen to carrier protein, while preserving both T-cell responses from carrier protein and B-cell responses from …
View article: Cryo-EM structures of prefusion SIV envelope trimer
Cryo-EM structures of prefusion SIV envelope trimer Open
View article: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability
Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability Open
View article: Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron Open
Since the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some of these variants show increased resistance to vaccine-elicited …
View article: Structure of an influenza group 2-neutralizing antibody targeting the hemagglutinin stem supersite
Structure of an influenza group 2-neutralizing antibody targeting the hemagglutinin stem supersite Open
View article: Prefusion-Stabilized Lassa Virus Trimer Identifies Neutralizing Nanobodies and Reveals an Apex-Situated Site of Vulnerability
Prefusion-Stabilized Lassa Virus Trimer Identifies Neutralizing Nanobodies and Reveals an Apex-Situated Site of Vulnerability Open
Lassa virus (LASV) is responsible for 100,000-300,000 zoonotic infections annually and poses a threat to public health. Development of antibody-based therapeutics or vaccines has been challenging because neutralizing antibodies – even amon…
View article: Structural basis for llama nanobody recognition and neutralization of HIV-1 at the CD4-binding site
Structural basis for llama nanobody recognition and neutralization of HIV-1 at the CD4-binding site Open
View article: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability
Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability Open
Summary Immunization with SARS-CoV-2 spike elicits diverse antibodies, but can any of these neutralize broadly? Here, we report the isolation and characterization of antibody WS6, from a mouse immunized with mRNA encoding the SARS-CoV-2 sp…
View article: Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron Open
Since the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some of these variants show increased resistance to vaccines, convale…
View article: Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies
Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies Open