Silvio O. Rizzoli
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View article: Impaired Atrial Mitochondrial Calcium Handling in Patients With Atrial Fibrillation
Impaired Atrial Mitochondrial Calcium Handling in Patients With Atrial Fibrillation Open
BACKGROUND: Mitochondrial calcium (Ca 2+ ) is a key regulator of cardiac energetics by stimulating the tricarboxylic acid cycle during elevated workload. Atrial fibrillation (AF) is associated with a reduction in cytosolic Ca 2+ transient …
View article: Publisher Correction: Functional synapses between neurons and small cell lung cancer
Publisher Correction: Functional synapses between neurons and small cell lung cancer Open
View article: Functional synapses between neurons and small cell lung cancer
Functional synapses between neurons and small cell lung cancer Open
View article: Neddylation regulates the development and function of glutamatergic neurons
Neddylation regulates the development and function of glutamatergic neurons Open
View article: SynapseNet: Deep learning for automatic synapse reconstruction
SynapseNet: Deep learning for automatic synapse reconstruction Open
Electron microscopy is an important technique for the study of synaptic morphology and its relation to synaptic function. The data analysis for this task requires the segmentation of the relevant synaptic structures, such as synaptic vesic…
View article: Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization
Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization Open
Neuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Re…
View article: Glutamatergic synaptic resilience to overexpressed human alpha-synuclein
Glutamatergic synaptic resilience to overexpressed human alpha-synuclein Open
Alpha synuclein (aSyn) is abundant in the brain and strongly implicated in Parkinson’s disease (PD), genetically and through its accumulation in neuronal pathognomonic inclusions. While mutations or increased expression of wild-type aSyn c…
View article: Geometry effects on protein mobility in a synapse
Geometry effects on protein mobility in a synapse Open
It is generally assumed that synaptic function requires a tight regulation of the mobility and localization of synaptic proteins. Evidence for this hypothesis has been difficult to gather. Protein mobility can be measured via fluorescence …
View article: Long-term potentiation-induced changes in actin dynamics and spine geometry persist on the timescale of the synaptic tag
Long-term potentiation-induced changes in actin dynamics and spine geometry persist on the timescale of the synaptic tag Open
According to the tagging and capture hypothesis, long-lasting long-term potentiation (LTP) requires protein synthesis and a synaptic tag, which is a synapse specific memory of the stimulus with a so far unclear molecular or biophysical ide…
View article: Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle
Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle Open
Synaptic transmission is driven by a complex cycle of vesicle docking, release, and recycling, maintained by distinct vesicle pools. However, the partitioning of vesicle pools and reserve pool recruitment remain poorly understood. We use a…
View article: Endogenous SNAP-Tagging of Munc13-1 for Monitoring Synapse Nanoarchitecture
Endogenous SNAP-Tagging of Munc13-1 for Monitoring Synapse Nanoarchitecture Open
Synaptic function is governed by highly regulated protein machineries, whose abundance and spatial localization change continually. Studies to determine dynamic changes in synaptic protein nanoarchitecture typically rely on immunolabeling …
View article: Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin
Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin Open
The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonethel…
View article: Nanobinders for Synaptotagmin 1 enable the analysis of synaptic vesicle dynamics in rodent and human models
Nanobinders for Synaptotagmin 1 enable the analysis of synaptic vesicle dynamics in rodent and human models Open
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes to be affected in neural diseases. Monitoring this process, and in particular synaptic vesicle recycling, in living cells has been instrumen…
View article: Power-law adaptation in the presynaptic vesicle cycle
Power-law adaptation in the presynaptic vesicle cycle Open
View article: Microtubules as a versatile reference standard for expansion microscopy
Microtubules as a versatile reference standard for expansion microscopy Open
View article: PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis
PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis Open
Neuronal communication relies on synaptic vesicle recycling, which has long been investigated by live imaging approaches. Synapto-pHluorins, genetically encoded reporters that incorporate a pH-sensitive variant of GFP within the lumen of t…
View article: Ca2+-Triggered (de)ubiquitination Events in Synapses
Ca2+-Triggered (de)ubiquitination Events in Synapses Open
Neuronal communication relies on neurotransmitter release from synaptic vesicles (SVs), whose dynamics are controlled by Ca2+-dependent pathways, as many thoroughly studied phosphorylation cascades. However, little is known abou…
View article: A correlative workflow streamlines synaptic imaging by cryo-electron tomography
A correlative workflow streamlines synaptic imaging by cryo-electron tomography Open
Despite decades of intense research, the molecular organization of the synapse is not well understood. To address this issue, we sought to develop a method for systematic imaging of synapses by cryo-electron tomography (cryo-ET), a technol…
View article: Super-resolution Live-cell Fluorescence Lifetime Imaging
Super-resolution Live-cell Fluorescence Lifetime Imaging Open
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM …
View article: Glutamatergic synaptic resilience to overexpressed human alpha-synuclein
Glutamatergic synaptic resilience to overexpressed human alpha-synuclein Open
Alpha synuclein (aSyn) is an abundant protein that, in the brain, is concentrated in neuronal presynaptic boutons and associates with synaptic vesicles. aSyn is strongly linked with Parkinson’s disease (PD) due to its accumulation in patho…
View article: How energy determines spatial localisation and copy number of molecules in neurons
How energy determines spatial localisation and copy number of molecules in neurons Open
In neurons, the quantities of mRNAs and proteins are traditionally assumed to be determined by functional, electrical or genetic factors. Yet, there may also be global, currently unknown computational rules that are valid across different …
View article: Geometry effects on protein mobility in a synapse
Geometry effects on protein mobility in a synapse Open
It is generally assumed that synaptic function requires a tight regulation of the mobility and localisation of synaptic proteins. Evidence for this hypothesis has been difficult to gather. Protein mobility can be measured via fluorescence …
View article: A microscopy-based screen identifies cellular kinases modulating mitochondrial translation
A microscopy-based screen identifies cellular kinases modulating mitochondrial translation Open
Mitochondrial DNA encodes 13 subunits of the oxidative phosphorylation (OXPHOS) system, which are synthesized inside the organelle and essential for cellular energy supply. How mitochondrial gene expression is regulated and integrated into…
View article: SynapseNet: Deep Learning for Automatic Synapse Reconstruction
SynapseNet: Deep Learning for Automatic Synapse Reconstruction Open
Electron microscopy is an important technique for the study of synaptic morphology and its relation to synaptic function. The data analysis for this task requires the segmentation of the relevant synaptic structures, such as synaptic vesic…
View article: MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity
MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity Open
α-Synuclein proximity ligation assay (PLA) has proved a sensitive technique for detection of non-Lewy body α-synuclein aggregate pathology. Here, we describe the MJF-14 PLA, a new PLA towards aggregated α-synuclein with unprecedented speci…
View article: Endogenous SNAP-tagging of Munc13-1 for monitoring synapse nanoarchitecture
Endogenous SNAP-tagging of Munc13-1 for monitoring synapse nanoarchitecture Open
Synaptic function is governed by highly regulated protein machineries, whose abundance and spatial localization change continually. Studies to determine the dynamic changes in synaptic proteins nanoarchitecture typically rely on immunolabe…
View article: NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals Open
Fluorescence microscopy has long been a transformative technique in biological sciences. Nevertheless, most implementations are limited to a few targets, which have been revealed using primary antibodies and fluorescently conjugated second…
View article: One-step nanoscale expansion microscopy reveals individual protein shapes
One-step nanoscale expansion microscopy reveals individual protein shapes Open
View article: The synaptic vesicle cluster as a controller of pre‐ and postsynaptic structure and function
The synaptic vesicle cluster as a controller of pre‐ and postsynaptic structure and function Open
The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which provides neurotransmitter‐loaded vesicles during synaptic activity, at the same time as also controlling the local concentrations of numerous exo‐ and…
View article: Membrane trafficking of synaptic adhesion molecules
Membrane trafficking of synaptic adhesion molecules Open
Synapse formation and stabilization are aided by several families of adhesion molecules, which are generally seen as specialized surface receptors. The function of most surface receptors, including adhesion molecules, is modulated in non‐n…