Šimon Džatko
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DNA i-motif levels are overwhelmingly depleted in living human cells: insights from in-cell NMR. Open
I-Motifs (iM) are non-canonical DNA structures potentially forming in the accessible, single-stranded, cytosine-rich genomic regions with regulatory roles. Chromatin, protein interactions, and intracellular properties seem to govern iM for…
DNA i-motif levels are overwhelmingly depleted in living human cells: insights from in-cell NMR Open
I-Motifs (iM) are non-canonical DNA structures potentially forming in accessible, single-stranded, cytosine-rich genomic regions, with regulatory roles. Chromatin, protein interactions, and intracellular properties seem to govern iM format…
Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy Open
Recently, the 1H-detected in-cell NMR spectroscopy has emerged as a unique tool allowing the characterization of interactions between nucleic acid-based targets and drug-like molecules in living human cells. Here, we assess the application…
In‐Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells Open
We report here the in‐cell NMR‐spectroscopic observation of the binding of the cognate ligand 2′‐deoxyguanosine to the aptamer domain of the bacterial 2′‐deoxyguanosine‐sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes …
In‐Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells Open
We report here the in‐cell NMR‐spectroscopic observation of the binding of the cognate ligand 2′‐deoxyguanosine to the aptamer domain of the bacterial 2′‐deoxyguanosine‐sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes …
Monitoring DNA–Ligand Interactions in Living Human Cells Using NMR Spectroscopy Open
Studies on DNA-ligand interactions in the cellular environment are problematic due to the lack of suitable biophysical tools. To address this need, we developed an in-cell NMR-based approach for monitoring DNA-ligand interactions inside th…
Monitoring DNA-Ligand Interactions in Living Human Cells Using High-Resolution NMR Spectroscopy Open
High-resolution studies of DNA–ligand interactions in the cellular environment are problematic due to the lack of suitable biophysical tools. To address this issue, we developed an in-cell NMR-based approach for monitoring DNA–ligand inter…
Monitoring DNA-Ligand Interactions in Living Human Cells Using High-Resolution NMR Spectroscopy Open
High-resolution studies of DNA–ligand interactions in the cellular environment are problematic due to the lack of suitable biophysical tools. To address this issue, we developed an in-cell NMR-based approach for monitoring DNA–ligand inter…
Evaluation of the Stability of DNA i‐Motifs in the Nuclei of Living Mammalian Cells Open
C‐rich DNA has the capacity to form a tetra‐stranded structure known as an i‐motif. The i‐motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the exis…
Evaluation of the Stability of DNA i‐Motifs in the Nuclei of Living Mammalian Cells Open
C‐rich DNA has the capacity to form a tetra‐stranded structure known as an i‐motif. The i‐motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the exis…