Simon E. Tröder
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View article: Furin-like cleavage at the C1-C2 linker region of the ⍺3 chain is not required for collagen VI assembly
Furin-like cleavage at the C1-C2 linker region of the ⍺3 chain is not required for collagen VI assembly Open
Collagen VI is a heterotrimeric, ubiquitously expressed microfibrillar collagen with a complex intracellular and extracellular assembly process. In addition to a short collagenous region, it is primarily composed of von Willebrand factor A…
View article: A red fluorescent lifeact marker to study actin morphology in podocytes
A red fluorescent lifeact marker to study actin morphology in podocytes Open
F-actin is a major component of the cellular cytoskeleton, responsible for maintaining cell shape, enabling movement and facilitating intracellular transport. In the kidney, glomerular podocytes are highly dependent on their actin cytoskel…
View article: Defective mitochondrial COX1 translation due to loss of COX14 function triggers ROS-induced inflammation in mouse liver
Defective mitochondrial COX1 translation due to loss of COX14 function triggers ROS-induced inflammation in mouse liver Open
Mitochondrial oxidative phosphorylation (OXPHOS) fuels cellular ATP demands. OXPHOS defects lead to severe human disorders with unexplained tissue specific pathologies. Mitochondrial gene expression is essential for OXPHOS biogenesis since…
View article: Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization
Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization Open
Objective: The ever-increasing number of genetically engineered mouse models highlights the need for efficient archiving and distribution of these lines. Sperm cryopreservation has become the preferred technique for the majority of these m…
View article: Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization
Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization Open
Objective: The ever-increasing number of genetically engineered mouse models highlights the need for efficient archiving and distribution of these lines. Sperm cryopreservation has become the preferred technique for the majority of these m…
View article: SARM1 deletion delays cerebellar but not spinal cord degeneration in an enhanced mouse model of <i>SPG7</i> deficiency
SARM1 deletion delays cerebellar but not spinal cord degeneration in an enhanced mouse model of <i>SPG7</i> deficiency Open
Hereditary spastic paraplegia is a neurological condition characterized by predominant axonal degeneration in long spinal tracts, leading to weakness and spasticity in the lower limbs. The nicotinamide adenine dinucleotide (NAD+)-consuming…
View article: Successful use of HTF as basal fertilization medium during SEcuRe mouse in vitro fertilization
Successful use of HTF as basal fertilization medium during SEcuRe mouse in vitro fertilization Open
Objective: The ever-increasing number of genetically engineered mouse models highlights the need for efficient archiving and distribution of these lines. Sperm cryopreservation has become the preferred technique for the majority of these m…
View article: Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathy
Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathy Open
De novo mutations in voltage- and ligand-gated channels have been associated with an increasing number of cases of developmental and epileptic encephalopathies, which often fail to respond to classic antiseizure medications. Here, we exami…
View article: Author response: Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathy
Author response: Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathy Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract De novo mutations in v…
View article: Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v4
Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v4 Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm
A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm Open
The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to em…
View article: A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v2
A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v2 Open
The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to em…
View article: Seizures, behavioral deficits and adverse drug responses in two new genetic mouse models of <i>HCN1</i> epileptic encephalopathy
Seizures, behavioral deficits and adverse drug responses in two new genetic mouse models of <i>HCN1</i> epileptic encephalopathy Open
De novo mutations in voltage- and ligand-gated channels have been associated with an increasing number of cases of developmental and epileptic encephalopathies, which often fail to respond to classic antiseizure medications. Here, we exami…
View article: Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v2
Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v2 Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v3
Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v3 Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v1
A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v1 Open
The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to em…
View article: An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes
An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes
An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v1
Simple electroporation for efficient CRISPR/Cas9 genome editing in murine zygotes v1 Open
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in spec…
View article: Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria
Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria Open
Proteolytic cleavage of the dynamin-like guanosine triphosphatase OPA1 in mitochondria is emerging as a central regulatory hub that determines mitochondrial morphology under stress and in disease. Stress-induced OPA1 processing by OMA1 tri…