Simon Umbach
YOU?
Author Swipe
View article: RIM and RIM-Binding Protein Localize Synaptic CaV2 Channels to Differentially Regulate Transmission in Neuronal Circuits
RIM and RIM-Binding Protein Localize Synaptic CaV2 Channels to Differentially Regulate Transmission in Neuronal Circuits Open
At chemical synapses, voltage-gated Ca 2+ channels (VGCCs) translate electrical signals into a trigger for synaptic vesicle (SV) fusion. VGCCs and the Ca 2+ microdomains they elicit must be located precisely to primed SVs to evoke rapid tr…
View article: Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment
Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment Open
Here we describe the cryo-electron microscopy structure of the human histamine 2 receptor (H 2 R) in an active conformation with bound histamine and in complex with G s heterotrimeric protein at an overall resolution of 3.4 Å. The complex …
View article: Nanodelivery: a novel approach to transfer cell-free expressed GPCRs into live cells
Nanodelivery: a novel approach to transfer cell-free expressed GPCRs into live cells Open
In biochemistry and cell biology, DNA/RNA transfection is one of the most fundamental and essential methods for modifying cellular phenotypes. This technique is critically important because it enables the study of protein function and regu…
View article: Cryo-EM structure of cell-free synthesized human histamine H<sub>2</sub>receptor coupled to heterotrimeric G<sub>s</sub>protein in lipid nanodisc environment
Cryo-EM structure of cell-free synthesized human histamine H<sub>2</sub>receptor coupled to heterotrimeric G<sub>s</sub>protein in lipid nanodisc environment Open
Here we describe the cryo-electron microscopy structure of the human histamine 2 receptor (H 2 R) in an active conformation with bound histamine and in complex with G s heterotrimeric protein at an overall resolution of 3.4 Å. The complex …
View article: Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells
Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells Open
Nanodiscs are emerging to serve as transfer vectors for the insertion of recombinant membrane proteins into membranes of living cells. In combination with cell-free expression technologies, this novel process opens new perspectives to anal…
View article: Ingestion and Toxicity of Polystyrene Microplastics in Freshwater Bivalves
Ingestion and Toxicity of Polystyrene Microplastics in Freshwater Bivalves Open
The ubiquity of microplastics in aquatic ecosystems has raised concerns over their interaction with biota. However, microplastics research on freshwater species, especially mollusks, is still scarce. We, therefore, investigated the factors…
View article: RIM and RIM-binding protein localize synaptic CaV2 channels to differentially regulate transmission in neuronal circuits
RIM and RIM-binding protein localize synaptic CaV2 channels to differentially regulate transmission in neuronal circuits Open
At chemical synapses, voltage-gated Ca 2+ -channels (VGCCs) translate electrical signals into a trigger for synaptic vesicle (SV) fusion. VGCCs and the Ca 2+ microdomains they elicit must be located precisely to primed SVs, to evoke rapid …