Simona Ranallo
YOU?
Author Swipe
View article: MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands
MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands Open
Here we report on the development of a CRISPR-based assay for the sensitive and specific detection of antibodies and antigens directly in complex sample matrices. The assay, called Molecular Assay based on antibody-Induced Guide-RNA Enzyma…
View article: MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands
MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands Open
Here we report on the development of a CRISPR-based assay for the sensitive and specific detection of antibodies and antigens directly in complex sample matrices. The assay, called MAIGRET (Molecular Assay based on antibody-Induced Guide-R…
View article: Developmental assembly of multi-component polymer systems through interconnected gene networks<i>in vitro</i>
Developmental assembly of multi-component polymer systems through interconnected gene networks<i>in vitro</i> Open
Living cells regulate the dynamics of developmental events through interconnected signaling systems that activate and deactivate inert precursors. This suggests that similarly, synthetic biomaterials could be designed to develop over time …
View article: Enzyme‐Linked DNA Displacement (ELIDIS) Assay for Ultrasensitive Electrochemical Detection of Antibodies**
Enzyme‐Linked DNA Displacement (ELIDIS) Assay for Ultrasensitive Electrochemical Detection of Antibodies** Open
Here we report the development of a method for the electrochemical ultrasensitive detection of antibodies that couples the programmability and versatility of DNA‐based systems with the sensitivity provided by enzymatic amplification. The p…
View article: Enzyme-Linked DNA Displacement (ELIDIS) Assay for Ultrasensitive Electrochemical Detection of Antibodies
Enzyme-Linked DNA Displacement (ELIDIS) Assay for Ultrasensitive Electrochemical Detection of Antibodies Open
Here we report the development of a method for the electrochemical ultrasensitive detection of antibodies that couples the programmability and versatility of DNA-based systems with the sensitivity provided by enzymatic amplification. The p…
View article: Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands Open
We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor…
View article: Co-transcriptional allosteric regulation of synthetic nucleic acid receptors
Co-transcriptional allosteric regulation of synthetic nucleic acid receptors Open
We present a strategy to control allosterically the loading and release of molecular ligands from synthetic nucleic acid receptors using in vitro transcription. We demonstrate this by engineering three model synthetic DNA-based receptors: …
View article: Synthetic Antigen-Conjugated DNA Systems for Antibody Detection and Characterization
Synthetic Antigen-Conjugated DNA Systems for Antibody Detection and Characterization Open
Antibodies are among the most relevant biomolecular targets for diagnostic and clinical applications. In this Perspective, we provide a critical overview of recent research efforts focused on the development and characterization of devices…
View article: Enzyme-DNA hybrids for ultrasensitive electrochemical detection of antibodies
Enzyme-DNA hybrids for ultrasensitive electrochemical detection of antibodies Open
Here we report a strategy for the electrochemical ultrasensitive detection of antibodies that couples the programmability and versatility of DNA-based systems with the sensitivity provided by enzymatic amplification. The platform is based …
View article: Cover Picture: Electrochemical Cell‐Free Biosensors for Antibody Detection (Angew. Chem. Int. Ed. 8/2023)
Cover Picture: Electrochemical Cell‐Free Biosensors for Antibody Detection (Angew. Chem. Int. Ed. 8/2023) Open
Programmable antigen-conjugated gene circuits, which, upon recognition of a target antibody, trigger the cell-free transcription of an RNA sequence that can be consequently detected with a disposable electrochemical sensor, were designed b…
View article: Using Spectroscopy to Guide the Adaptation of Aptamers into Electrochemical Aptamer-Based Sensors
Using Spectroscopy to Guide the Adaptation of Aptamers into Electrochemical Aptamer-Based Sensors Open
Electrochemical aptamer-based (EAB) sensors utilize the binding-induced conformational change of an electrode-attached, redox-reporter-modified aptamer to transduce target recognition into an easily measurable electrochemical output. Becau…
View article: Programmable Cell-Free Transcriptional Switches for Antibody Detection
Programmable Cell-Free Transcriptional Switches for Antibody Detection Open
We report here the development of a cell-free in vitro transcription system for the detection of specific target antibodies. The approach is based on the use of programmable antigen-conjugated DNA-based conformational switches that, upon b…
View article: Rücktitelbild: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice (Angew. Chem. 12/2022)
Rücktitelbild: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice (Angew. Chem. 12/2022) Open
Die Informationsverarbeitung in lebenden Zellen beruht auf der Kommunikation zwischen DNA, RNA und Proteinen. Inspiriert von diesen molekularen Kommunikationssystemen demonstrieren Simona Ranallo, Francesco Ricci et al. in ihrem Forschungs…
View article: Back Cover: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice (Angew. Chem. Int. Ed. 12/2022)
Back Cover: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice (Angew. Chem. Int. Ed. 12/2022) Open
Information processing in living cells results from the communication between DNA, RNA, and proteins. Drawing inspiration from these molecular communication systems, Simona Ranallo, Francesco Ricci, and co-workers describe in their Communi…
View article: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice**
Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice** Open
We report here the rational design and optimization of an antibody‐responsive, DNA‐based device that enables communication between pairs of otherwise non‐interacting proteins. The device is designed to recognize and bind a specific antibod…
View article: Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice**
Protein–Protein Communication Mediated by an Antibody‐Responsive DNA Nanodevice** Open
We report here the rational design and optimization of an antibody‐responsive, DNA‐based device that enables communication between pairs of otherwise non‐interacting proteins. The device is designed to recognize and bind a specific antibod…
View article: Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device
Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device Open
We report here the rational design and optimization of an antibody responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibod…
View article: Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device
Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device Open
We report here the rational design and optimization of an antibody responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibod…
View article: Programmable cell-free transcriptional switches for antibodies detection
Programmable cell-free transcriptional switches for antibodies detection Open
We report here the development of a cell-free in-vitro transcription system for the detection of specific target antibodies. The approach is based on the use of programmable antigen-conjugated DNA-based conformational switches that, upon b…
View article: Programmable cell-free transcriptional switches for antibodies detection
Programmable cell-free transcriptional switches for antibodies detection Open
We report here the development of a cell-free in-vitro transcription system for the detection of specific target antibodies. The approach is based on the use of programmable antigen-conjugated DNA-based conformational switches that, upon b…
View article: Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection
Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection Open
Current health emergencies have highlighted the need to have rapid, sensitive, and convenient platforms for the detection of specific antibodies. In response, we report here the design of an electrochemical DNA circuit that responds quanti…
View article: Rational Control of the Activity of a Cu<sup>2+</sup>-Dependent DNAzyme by Re-engineering Purely Entropic Intrinsically Disordered Domains
Rational Control of the Activity of a Cu<sup>2+</sup>-Dependent DNAzyme by Re-engineering Purely Entropic Intrinsically Disordered Domains Open
The function and activity of many proteins is finely controlled by the modulation of the entropic contribution of intrinsically disordered domains that are not directly involved in any recognition event. Inspired by this mechanism, we demo…
View article: DNA-Based Scaffolds for Sensing Applications
DNA-Based Scaffolds for Sensing Applications Open
DNA nanotechnology employs synthetic nucleic acid strands to design and engineer nanoscale structural and functional systems of increasing complexity that may find applications in sensing,1-7 computing,8-10 molecular transport,11-13 inform…