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View article: Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory
Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory Open
CRISPR/Cas9 genome editing is a rapidly advancing technology that has the potential to accelerate research and development in a variety of fields. However, manual genome editing processes suffer from limitations in scalability, efficiency,…
View article: Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell–derived sensory neurons
Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell–derived sensory neurons Open
Induced pluripotent stem cells (iPSCs) have enabled the generation of various difficult-to-access cell types such as human nociceptors. A key challenge associated with human iPSC-derived nociceptors (hiPSCdNs) is their prolonged functional…
View article: Dynamics of Metabolic Pathways and Stress Response Patterns during Human Neural Stem Cell Proliferation and Differentiation
Dynamics of Metabolic Pathways and Stress Response Patterns during Human Neural Stem Cell Proliferation and Differentiation Open
Understanding early nervous system stress response mechanisms is crucial for studying developmental neurotoxicity and devising neuroprotective treatments. We used hiPSC-derived long-term self-renewing neuroepithelial stem (lt-NES) cells di…
View article: Machine learning-aided multidimensional phenotyping of Parkinson’s disease patient stem cell-derived midbrain dopaminergic neurons
Machine learning-aided multidimensional phenotyping of Parkinson’s disease patient stem cell-derived midbrain dopaminergic neurons Open
Summary Combining multiple Parkinson’s disease (PD) relevant cellular phenotypes might increase the accuracy of midbrain dopaminergic (mDA) in vitro models. We differentiated patient-derived induced pluripotent stem cells (iPSCs) with a LR…
View article: Energy Metabolism Disturbances in Cell Models of PARK2 CNV Carriers with ADHD
Energy Metabolism Disturbances in Cell Models of PARK2 CNV Carriers with ADHD Open
The main goal of the present study was the identification of cellular phenotypes in attention-deficit-/hyperactivity disorder (ADHD) patient-derived cellular models from carriers of rare copy number variants (CNVs) in the PARK2 locus that …
View article: Deep-learning-based multi-class segmentation for automated, non-invasive routine assessment of human pluripotent stem cell culture status
Deep-learning-based multi-class segmentation for automated, non-invasive routine assessment of human pluripotent stem cell culture status Open
Human induced pluripotent stem cells (hiPSCs) are capable of differentiating into a variety of human tissue cells. They offer new opportunities for personalized medicine and drug screening. This requires large quantities of high quality hi…
View article: The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells
The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells Open
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic…
View article: Induced pluripotent stem cell‐based modeling of mutant <scp>LRRK</scp>2‐associated Parkinson's disease
Induced pluripotent stem cell‐based modeling of mutant <span>LRRK</span>2‐associated Parkinson's disease Open
Recent advances in cell reprogramming have enabled assessment of disease‐related cellular traits in patient‐derived somatic cells, thus providing a versatile platform for disease modeling and drug development. Given the limited access to v…
View article: Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays
Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays Open
In today's neurodevelopment and -disease research, human neural stem/progenitor cell-derived networks represent the sole accessible in vitro model possessing a primary phenotype. However, cultivation and moreover, differentiation as well a…