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View article: Front Cover: Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins (ChemBioChem 14/2025)
Front Cover: Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins (ChemBioChem 14/2025) Open
View article: In vivo haemopoietic stem cell gene therapy enabled by postnatal trafficking
In vivo haemopoietic stem cell gene therapy enabled by postnatal trafficking Open
Lentiviral vector (LV)-mediated ex vivo gene therapy for haematopoietic stem and progenitor cells (HSPCs) has delivered on the promise of a 'one-and-done' treatment for several genetic diseases1. However, ex vivo manipulation an…
View article: Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins
Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins Open
Designing artificial metal binding sites within a protein is challenging since amino acid residues need to be placed in desired positions in the final construct and the use of non‐natural amino acids is difficult. The alternative approach …
View article: Small molecules targeting the eubacterial β-sliding clamp discovered by combined <i>in silico</i> and <i>in vitro</i> screening approaches
Small molecules targeting the eubacterial β-sliding clamp discovered by combined <i>in silico</i> and <i>in vitro</i> screening approaches Open
Antibiotic resistance stands as the foremost post-pandemic threat to public health. The urgent need for new, effective antibacterial treatments is evident. Protein-protein interactions (PPIs), owing to their pivotal role in microbial physi…
View article: A safe and potentiated multi-type HPV L2-E7 nanoparticle vaccine with combined prophylactic and therapeutic activity
A safe and potentiated multi-type HPV L2-E7 nanoparticle vaccine with combined prophylactic and therapeutic activity Open
Persistent infection with high-risk human papillomavirus (HPV) is widely recognized as the primary cause of cervical and other malignant cancers. There are six licensed prophylactic vaccines available against HPV, but none of them shows an…
View article: Inter-epitope spacer variation within polytopic L2-based human papillomavirus antigens affects immunogenicity
Inter-epitope spacer variation within polytopic L2-based human papillomavirus antigens affects immunogenicity Open
View article: A dry powder formulation for peripheral lung delivery and absorption of an anti-SARS-CoV-2 ACE2 decoy polypeptide
A dry powder formulation for peripheral lung delivery and absorption of an anti-SARS-CoV-2 ACE2 decoy polypeptide Open
One of the strategies proposed for the neutralization of SARS-CoV-2 has been to synthetize small proteins able to act as a decoy towards the virus spike protein, preventing it from entering the host cells. In this work, the incorporation o…
View article: Time-course transcriptome analysis of a double challenge bleomycin-induced lung fibrosis rat model uncovers ECM homoeostasis-related translationally relevant genes
Time-course transcriptome analysis of a double challenge bleomycin-induced lung fibrosis rat model uncovers ECM homoeostasis-related translationally relevant genes Open
Background Idiopathic pulmonary fibrosis (IPF) is an irreversible disorder with a poor prognosis. The incomplete understanding of IPF pathogenesis and the lack of accurate animal models is limiting the development of effective treatments. …
View article: Natural heteroclitic-like peptides are generated by SARS-CoV-2 mutations
Natural heteroclitic-like peptides are generated by SARS-CoV-2 mutations Open
Humoral immunity is sensitive to evasion by SARS-CoV-2 mutants, but CD8 T cells seem to be more resistant to mutational inactivation. By a systematic analysis of 30 spike variant peptides containing the most relevant VOC and VOI mutations …
View article: Natural heteroclitic-like peptides are generated by SARS-CoV-2 mutations
Natural heteroclitic-like peptides are generated by SARS-CoV-2 mutations Open
Mutations carried by SARS-CoV-2 spike protein variants may promote viral escape from immune protection. Humoral immunity is sensitive to evasion by SARS-CoV-2 mutants, but the impact of viral evolution on the interplay between virus and ho…
View article: An Optimized Workflow for the Discovery of New Antimicrobial Compounds Targeting Bacterial RNA Polymerase Complex Formation
An Optimized Workflow for the Discovery of New Antimicrobial Compounds Targeting Bacterial RNA Polymerase Complex Formation Open
Bacterial resistance represents a major health problem worldwide and there is an urgent need to develop first-in-class compounds directed against new therapeutic targets. We previously developed a drug-discovery platform to identify new an…
View article: A broadly protective vaccine against cutaneous human papillomaviruses
A broadly protective vaccine against cutaneous human papillomaviruses Open
Skin colonization by human papillomavirus (HPV) is typically related to inconspicuous cutaneous infections without major disease or complications in immunocompetent individuals. However, in immunosuppressed patients, especially organ trans…
View article: Enhanced immunogenicity of a positively supercharged archaeon thioredoxin scaffold as a cell-penetrating antigen carrier for peptide vaccines
Enhanced immunogenicity of a positively supercharged archaeon thioredoxin scaffold as a cell-penetrating antigen carrier for peptide vaccines Open
Polycationic resurfaced proteins hold great promise as cell-penetrating bioreagents but their use as carriers for the intracellular delivery of peptide immuno-epitopes has not thus far been explored. Here, we report on the construction and…
View article: Degenerate CD8 Epitopes Mapping to Structurally Constrained Regions of the Spike Protein: A T Cell-Based Way-Out From the SARS-CoV-2 Variants Storm
Degenerate CD8 Epitopes Mapping to Structurally Constrained Regions of the Spike Protein: A T Cell-Based Way-Out From the SARS-CoV-2 Variants Storm Open
There is an urgent need for new generation anti-SARS-Cov-2 vaccines in order to increase the efficacy of immunization and its broadness of protection against viral variants that are continuously arising and spreading. The effect of variant…
View article: Broad Neutralization Responses Against Oncogenic Human Papillomaviruses Induced by a Minor Capsid L2 Polytope Genetically Incorporated Into Bacterial Ferritin Nanoparticles
Broad Neutralization Responses Against Oncogenic Human Papillomaviruses Induced by a Minor Capsid L2 Polytope Genetically Incorporated Into Bacterial Ferritin Nanoparticles Open
Cervical cancer remains a global health burden despite the introduction of highly effective vaccines for the prophylaxis of causative human papillomavirus infection (HPV). Current efforts to eradicate cervical cancer focus on the developme…
View article: Combined prophylactic and therapeutic immune responses against human papillomaviruses induced by a thioredoxin-based L2-E7 nanoparticle vaccine
Combined prophylactic and therapeutic immune responses against human papillomaviruses induced by a thioredoxin-based L2-E7 nanoparticle vaccine Open
Global burden of cervical cancer, the most common cause of mortality caused by human papillomavirus (HPV), is expected to increase during the next decade, mainly because current alternatives for HPV vaccination and cervical cancer screenin…
View article: Targeting p53 and histone methyltransferases to restore fully exhausted CD8+ T cells in human HCV infection before and after DAA-therapy
Targeting p53 and histone methyltransferases to restore fully exhausted CD8+ T cells in human HCV infection before and after DAA-therapy Open
View article: Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection
Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection Open
View article: New Antimicrobials Targeting Bacterial RNA Polymerase Holoenzyme Assembly Identified with an <i>in Vivo</i> BRET-Based Discovery Platform
New Antimicrobials Targeting Bacterial RNA Polymerase Holoenzyme Assembly Identified with an <i>in Vivo</i> BRET-Based Discovery Platform Open
Bacterial resistance represents a major health threat worldwide, and the development of new therapeutics, including innovative antibiotics, is urgently needed. We describe a discovery platform, centered on in silico screening and
View article: Pezizomycetes genomes reveal the molecular basis of ectomycorrhizal truffle lifestyle
Pezizomycetes genomes reveal the molecular basis of ectomycorrhizal truffle lifestyle Open
View article: Author Correction: A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mycorrhizal fungus Tuber melanosporum
Author Correction: A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mycorrhizal fungus Tuber melanosporum Open
View article: Broadly neutralizing antiviral responses induced by a single-molecule HPV vaccine based on thermostable thioredoxin-L2 multiepitope nanoparticles
Broadly neutralizing antiviral responses induced by a single-molecule HPV vaccine based on thermostable thioredoxin-L2 multiepitope nanoparticles Open
Vaccines targeting the human papillomavirus (HPV) minor capsid protein L2 are emerging as chemico-physically robust and broadly protective alternatives to the current HPV (L1-VLP) vaccines. We have previously developed a trivalent L2 vacci…
View article: Minor Capsid Protein L2 Polytope Induces Broad Protection against Oncogenic and Mucosal Human Papillomaviruses
Minor Capsid Protein L2 Polytope Induces Broad Protection against Oncogenic and Mucosal Human Papillomaviruses Open
The amino terminus of the human papillomavirus (HPV) minor capsid protein L2 contains a major cross-neutralization epitope which provides the basis for the development of a broadly protecting HPV vaccine. A wide range of protection against…
View article: Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor Open
View article: Correction: In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse
Correction: In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse Open
View article: A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mycorrhizal fungus Tuber melanosporum
A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mycorrhizal fungus Tuber melanosporum Open
View article: Additional file 6: Table S6. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Additional file 6: Table S6. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor Open
List of L. bicolor “core” TF orthologues genes in ECM fungi (A. muscaria; H. cylindrosporum; P. involutus, P. croceum, S. luteus, C. geophilum and T. melanospsorum). The shaded rows indicate orthologues (using Reciprocal Blast Hit) of L. b…
View article: Additional file 1: Table S1. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Additional file 1: Table S1. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor Open
List of L. bicolor TFs identified in silico displaying similarities with characterized TFs (183 proteins); sharing similarities with predicted or hypothetical proteins with no proved role as transcriptional regulators (94 proteins); sharin…
View article: Additional file 3: Table S3. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Additional file 3: Table S3. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor Open
Gene expression of L. bicolor TF-encoding genes that are significantly regulated during ECM development with Populus trichocarpa at 2, 4, 6 and/or 12Â weeks. Data used to generate Fig. 2b. (XLSX 26Â kb)
View article: Additional file 8: Table S8. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Additional file 8: Table S8. of Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor Open
List and sequences of primers used for qPCR. (XLSX 9Â kb)