Simone Reber
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View article: Adaptations in <i>Plasmodium</i> tubulin determine unique microtubule architectures, mechanics and drug susceptibility
Adaptations in <i>Plasmodium</i> tubulin determine unique microtubule architectures, mechanics and drug susceptibility Open
Microtubules are ubiquitous yet diverse cytoskeleton filaments. However, tubulin conservation presents challenges in understanding the origins of diverse microtubule architectures. The mechanisms by which microtubule architecture varies th…
View article: Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes
Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes Open
The confinement of macromolecules has profound implications for cellular biochemistry. It generates environments with specific physical properties affecting diffusion, macromolecular crowding, and reaction rates. Yet, it remains unknown ho…
View article: Hierarchical Heterogeneities in Spatio-Temporal Dynamics of the Cytoplasm
Hierarchical Heterogeneities in Spatio-Temporal Dynamics of the Cytoplasm Open
Understanding of the dynamics inherent to biological matter is crucial for illuminating the physical mechanisms underlying cellular processes. In this study, we employ bright-field differential dynamic microscopy (DDM) to investigate densi…
View article: Mechanistic basis of temperature adaptation in microtubule dynamics across frog species
Mechanistic basis of temperature adaptation in microtubule dynamics across frog species Open
Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities, this i…
View article: Mechanistic basis of temperature-adaptation in microtubule dynamics across frog species
Mechanistic basis of temperature-adaptation in microtubule dynamics across frog species Open
SUMMARY Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities…
View article: Cell State-Specific Cytoplasmic Material Properties Control Spindle Architecture and Scaling
Cell State-Specific Cytoplasmic Material Properties Control Spindle Architecture and Scaling Open
Mitotic spindles are dynamically intertwined with the cytoplasm they assemble in. How the physicochemical properties of the cytoplasm affect spindle architecture and size remains largely unknown. Using quantitative biochemistry in combinat…
View article: Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes
Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes Open
The packing and confinement of macromolecules in the cytoplasm and nucleoplasm has profound implications for cellular biochemistry. How intracellular density distributions vary and affect cellular physiology remains largely unknown. Here, …
View article: Calcitic Prisms of The Giant Seashell <i>Pinna Nobilis</i> Form Light Guide Arrays
Calcitic Prisms of The Giant Seashell <i>Pinna Nobilis</i> Form Light Guide Arrays Open
The shells of the Pinnidae family are based on a double layer of single‐crystal‐like calcitic prisms and inner aragonitic nacre, a structure known for its outstanding mechanical performance. However, on the posterior side, shells are missi…
View article: Predicting disordered regions driving phase separation of proteins under variable salt concentration
Predicting disordered regions driving phase separation of proteins under variable salt concentration Open
We investigate intrinsically disordered regions (IDRs) of phase separating proteins regarding their impact on liquid-liquid phase separation (LLPS) of the full protein. Our theoretical approach uses a mean-field theory that accounts for se…
View article: Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length
Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length Open
Nup98 FG repeat domains comprise hydrophobic FG motifs linked through uncharged spacers. FG motifs capture nuclear transport receptors (NTRs) during nuclear pore complex (NPC) passage, confer inter-repeat cohesion, and condense the domains…
View article: Predicting disordered regions driving phase separation of proteins under variable salt concentration
Predicting disordered regions driving phase separation of proteins under variable salt concentration Open
We investigate intrinsically disordered regions (IDRs) of phase separating proteins regarding their impact on liquid-liquid phase separation (LLPS) of the full protein. Our theoretical approach uses a mean-field theory that accounts for se…
View article: Calcitic prisms of the giant seashell<i>Pinna nobilis</i>form light guide arrays
Calcitic prisms of the giant seashell<i>Pinna nobilis</i>form light guide arrays Open
The shells of the Pinnidae family are based on a double layer of single-crystal-like calcitic prisms and inner aragonitic nacre, a structure known for its outstanding mechanical performance. However, on the posterior side, shells are missi…
View article: Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length
Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length Open
Nup98 FG repeat domains comprise hydrophobic FG motifs linked through uncharged spacers. FG motifs capture nuclear transport receptors (NTRs) during nuclear pore complex (NPC) passage, confer inter-repeat cohesion, and condense the domains…
View article: Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling
Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling Open
The function of cellular structures at the mesoscale is dependent on their geometry and proportionality to cell size. The mitotic spindle is a good example why length and shape of intracellular organelles matter. Spindle length determines …
View article: Purification of functional <i>Plasmodium falciparum</i> tubulin allows for the identification of parasite-specific microtubule inhibitors
Purification of functional <i>Plasmodium falciparum</i> tubulin allows for the identification of parasite-specific microtubule inhibitors Open
Cytoskeletal proteins are essential for parasite proliferation, growth, and transmission, and therefore represent promising drug targets. While αβ-tubulin, the molecular building block of microtubules, is an established drug target in a va…
View article: Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling
Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling Open
The function of cellular structures at the mesoscale is dependent on their geometry and proportionality to cell size. The mitotic spindle is a good example why length and shape of intracellular organelles matter. Spindle length determines …
View article: The effects of proliferation status and cell cycle phase on the responses of single cells to chemotherapy
The effects of proliferation status and cell cycle phase on the responses of single cells to chemotherapy Open
DNA-damaging chemotherapy often leaves residual tumor cells. Combining single-cell long-term live imaging with information theory, we found an unexpected effect: highly proliferative cells were more likely to arrest than to die, whereas mo…
View article: MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules
MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules Open
Microtubules are polar, dynamic filaments fundamental to many cellular processes. In vitro reconstitution approaches with purified tubulin are essential to elucidate different aspects of microtubule behavior. To date, deriving data from fl…