Sheila K. Singh
YOU?
Author Swipe
View article: TMIC-51. D-serine facilitates aggressive migration and stemness of recurrent glioblastoma cells by interacting with host endothelial cells
TMIC-51. D-serine facilitates aggressive migration and stemness of recurrent glioblastoma cells by interacting with host endothelial cells Open
INTRODUCTION Glioblastoma (GBM) cells infiltrate deep brain structures by exploiting perivascular pathways, utilizing stem-like properties and malignant traits to access nutrient-rich environments that support tumor expansion. Our findings…
View article: TMIC-15. Reactive Oligodendrocytes Promote Glioblastoma Progression via CCL5/CCR5-Mediated Glioma Stem Cell Maintenance
TMIC-15. Reactive Oligodendrocytes Promote Glioblastoma Progression via CCL5/CCR5-Mediated Glioma Stem Cell Maintenance Open
BACKGROUND Glioblastoma (GBM) progression is tightly linked to microenvironmental interactions that remain poorly defined. Oligodendrocyte-lineage cells (OLs), traditionally viewed as passive bystanders, are increasingly recognized in the …
View article: Investigating the cytotoxicity of expanded NK and CD70-targeting CAR-NK cells against brain tumor cells 3722
Investigating the cytotoxicity of expanded NK and CD70-targeting CAR-NK cells against brain tumor cells 3722 Open
Description Glioblastoma multiforme (GBM) and medulloblastoma (MB) are aggressive brain cancers that demonstrate poor patient survival despite intense interventions. Natural Killer (NK) cells are promising in cancer immunotherapy due to th…
View article: BIOM-13. A GENOME-WIDE IN VIVO CRISPR ACTIVATION SCREEN IDENTIFIES BACE1 AS A THERAPEUTIC VULNERABILITY OF LUNG CANCER BRAIN METASTASIS
BIOM-13. A GENOME-WIDE IN VIVO CRISPR ACTIVATION SCREEN IDENTIFIES BACE1 AS A THERAPEUTIC VULNERABILITY OF LUNG CANCER BRAIN METASTASIS Open
Brain metastasis occurs in up to 40% of patients with non-small cell lung cancer (NSCLC). Considerable genomic heterogeneity exists between the primary lung tumor and respective brain metastasis; however, the identity of the genes capable …
View article: EXTH-142. Quantitative surfaceome profiling of high-risk medulloblastoma prioritizes the oncofetal antigen GPC2 for potent CAR-T cell therapy
EXTH-142. Quantitative surfaceome profiling of high-risk medulloblastoma prioritizes the oncofetal antigen GPC2 for potent CAR-T cell therapy Open
Recurrent medulloblastoma (MB) remains a devastating pediatric brain tumor with <10% survival. CAR-T cells have shown promise in aggressive childhood brain tumors, but success in MB is limited by suitable cell-surface targets. Requireme…
View article: Advancing Cancer Biology: Highlights from the 2025 FASEB SRC on Cellular Plasticity in Cancer
Advancing Cancer Biology: Highlights from the 2025 FASEB SRC on Cellular Plasticity in Cancer Open
The inaugural FASEB Science Research Conference (SRC) on Cellular Plasticity in Cancer was held in May 2025 in Hong Kong SAR, China. This event brought together leading experts to discuss cutting-edge research centered on cancer cell plast…
View article: P10.24.A TARGETING NON-GENETIC KINASE-DEPENDENT SIGNALING TO PREVENT GROUP 3 MEDULLOBLASTOMA RECURRENCE
P10.24.A TARGETING NON-GENETIC KINASE-DEPENDENT SIGNALING TO PREVENT GROUP 3 MEDULLOBLASTOMA RECURRENCE Open
BACKGROUND Brain tumors are the leading cause of cancer-related death in children, with medulloblastoma (MB) comprising 20-25% of cases. Proteogenomic profiling categorizes MB into four subgroups: Wingless (WNT), Sonic Hedgehog (SHH), Grou…
View article: 29 SNAT2 AS A NOVEL TARGETABLE VULNERABILITY IN RECURRENT GLIOBLASTOMA
29 SNAT2 AS A NOVEL TARGETABLE VULNERABILITY IN RECURRENT GLIOBLASTOMA Open
Glioblastoma (GBM) is the most fatal primary brain tumour. Standard-of-care consists of surgical resection followed by chemo-radiotherapy, but the tumour almost always recurs. There are currently no effective treatments at recurrence, and …
View article: 27 GENETIC SCREENING REVEALS FEN1 AS A DRIVER OF STEMNESS AND TEMOZOLOMIDE RESISTANCE IN GLIOBLASTOMA
27 GENETIC SCREENING REVEALS FEN1 AS A DRIVER OF STEMNESS AND TEMOZOLOMIDE RESISTANCE IN GLIOBLASTOMA Open
Glioblastoma (GBM) is a highly aggressive brain tumor with limited response to standard of care chemoradiotherapy. In this study, we conducted genome-wide CRISPR knockout screening in patient-derived glioblastoma stem cells (GSCs) to ident…
View article: 9 D-SERINE FACILITATES AGGRESSIVE MIGRATION AND STEMNESS OF RECURRENT GLIOBLASTOMA CELLS BY INTERACTING WITH HOST ENDOTHELIAL CELLS
9 D-SERINE FACILITATES AGGRESSIVE MIGRATION AND STEMNESS OF RECURRENT GLIOBLASTOMA CELLS BY INTERACTING WITH HOST ENDOTHELIAL CELLS Open
Sponsored by BC Cancer Foundation Introduction: Glioblastoma (GBM) cells infiltrate deep brain structures by exploiting perivascular pathways, utilizing stem-like properties and malignant traits to access nutrient-rich environments that su…
View article: PRCA-06 GENOME-WIDE IN VIVO CRISPR ACTIVATION SCREEN IDENTIFIES BACE1 AS A THERAPEUTIC VULNERABILITY OF LUNG CANCER BRAIN METASTASIS
PRCA-06 GENOME-WIDE IN VIVO CRISPR ACTIVATION SCREEN IDENTIFIES BACE1 AS A THERAPEUTIC VULNERABILITY OF LUNG CANCER BRAIN METASTASIS Open
Brain metastasis occurs in up to 40% of patients with non-small cell lung cancer (NSCLC). Considerable genomic heterogeneity exists between the primary lung tumor and respective brain metastasis; however, the identity of the genes capable …
View article: A genome-wide in vivo CRISPR activation screen identifies BACE1 as a therapeutic vulnerability of lung cancer brain metastasis
A genome-wide in vivo CRISPR activation screen identifies BACE1 as a therapeutic vulnerability of lung cancer brain metastasis Open
Brain metastasis occurs in up to 40% of patients with non–small cell lung cancer (NSCLC). Considerable genomic heterogeneity exists between the primary lung tumor and respective brain metastasis; however, the identity of the genes capable …
View article: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity
Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity Open
Factors influencing the macrophage surfaceome define macrophage identity and behavior. Here, we use genome-wide phenotypic screens to identify genes affecting the accessibility and surface expression of macrophage signal regulatory protein…
View article: Integrated genetic screening reveals FEN1 as a driver of stemness and temozolomide resistance in glioblastoma
Integrated genetic screening reveals FEN1 as a driver of stemness and temozolomide resistance in glioblastoma Open
Glioblastoma (GBM) is a lethal brain tumor with limited response to standard of care chemoradiotherapy. In this study, we conducted genome-wide CRISPR knockout screening in patient-derived glioblastoma stem cells (GSCs) to identify genetic…
View article: DAP12-associated synthetic antigen receptors enable multi-targeting of T cells with independent chimeric receptors in a small genetic payload
DAP12-associated synthetic antigen receptors enable multi-targeting of T cells with independent chimeric receptors in a small genetic payload Open
We describe a series of DAP12-associated receptors that can be used to achieve multi-targeting within a small genetic payload. Empirical evaluation of scaffold/binder combinations is required to define the optimal synthetic receptor config…
View article: Disease stage-specific role of the mitochondrial pyruvate carrier suppresses differentiation in temozolomide and radiation-treated glioblastoma
Disease stage-specific role of the mitochondrial pyruvate carrier suppresses differentiation in temozolomide and radiation-treated glioblastoma Open
Background The mitochondrial pyruvate carrier (MPC), a central metabolic conduit linking glycolysis and mitochondrial metabolism, is instrumental in energy production. However, the role of the MPC in cancer is controversial. In particular,…
View article: Pan-cancer<i>N</i>-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as a therapeutic target for head and neck cancers
Pan-cancer<i>N</i>-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as a therapeutic target for head and neck cancers Open
SUMMARY Cell surface proteins offer significant cancer therapeutic potential attributable to their accessible membrane localization and central role in cellular signaling. Despite this, their promise remains largely untapped due to the tec…
View article: TMET-24. MULTI-OMIC CHARACTERIZATION OF THE TEMPORAL METABOLIC ADAPTATIONS UNDERPINNING THERAPY RESISTANCE AND TUMOR RECURRENCE IN GLIOBLASTOMA
TMET-24. MULTI-OMIC CHARACTERIZATION OF THE TEMPORAL METABOLIC ADAPTATIONS UNDERPINNING THERAPY RESISTANCE AND TUMOR RECURRENCE IN GLIOBLASTOMA Open
Therapy resistance and tumor recurrence are barriers to achieving long-term survival in cancer patients, particularly evident in glioblastoma (GBM), known for its dismal 5-year survival rate below 10%. Despite aggressive treatment regimens…
View article: EXTH-51. ENHANCING GLIOBLASTOMA TREATMENT: ALLOGENEIC CAR-T CELLS OVERCOMES FUNCTIONAL DEFICITS OF PATIENT-DERIVED AUTOLOGOUS PRODUCTS
EXTH-51. ENHANCING GLIOBLASTOMA TREATMENT: ALLOGENEIC CAR-T CELLS OVERCOMES FUNCTIONAL DEFICITS OF PATIENT-DERIVED AUTOLOGOUS PRODUCTS Open
Glioblastoma (GBM) is the most common primary brain malignancy in adults, with a dismal prognosis despite an intensive standard of care. Recently, chimeric antigen receptor T-cell (CAR-T) therapy has shown promising outcomes in treating li…
View article: DDDR-42. LEVERAGING GENOME-WIDE CRISPR/CAS9 KNOCKOUT DRUG SCREENS TO IDENTIFY SENSITIZERS FOR PROTEOSOME INHIBITORS IN GLIOBLASTOMA
DDDR-42. LEVERAGING GENOME-WIDE CRISPR/CAS9 KNOCKOUT DRUG SCREENS TO IDENTIFY SENSITIZERS FOR PROTEOSOME INHIBITORS IN GLIOBLASTOMA Open
Glioblastoma (GBM) is the most common primary brain tumor with a poor prognosis despite aggressive treatment. Patient outcomes remain abysmal with 95% of patients relapsing and a median overall survival of 15 months. This necessitates the …
View article: IMMU-65. TARGETING AXONAL GUIDANCE DEPENDENCIES IN GLIOBLASTOMA WITH ROBO1 CAR T CELLS
IMMU-65. TARGETING AXONAL GUIDANCE DEPENDENCIES IN GLIOBLASTOMA WITH ROBO1 CAR T CELLS Open
Resistance to genotoxic therapies and subsequent disease recurrence are hallmarks of aggressive cancers, including glioblastoma (GBM). Here, we uncover functional drivers of post-treatment recurrent GBM using genome scale CRISPR-Cas9 scree…
View article: TMET-34. PYROPTOSIS IS AN ACQUIRED VULNERABILITY OF BRAIN METASTASES
TMET-34. PYROPTOSIS IS AN ACQUIRED VULNERABILITY OF BRAIN METASTASES Open
Metastasis, the spread of cancer cells from one part of the body to another, is a leading cause of death among cancer patients. Three particularly dangerous forms of metastases are the spread of lung, skin, and breast cancers to the brain.…