Siwei Chu
YOU?
Author Swipe
View article: Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to Detect Newly Synthesized Proteins in Cells and their Secretome v1
Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to Detect Newly Synthesized Proteins in Cells and their Secretome v1 Open
Cells respond to physiological or pathological stimuli by altering the composition of the proteins they produce. This adaptation includes changes to newly translated polypeptides that are destined for intracellular compartments or secretio…
View article: Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome.
Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome. Open
Our work demonstrates the power of a modified BONCAT protocol to study newly produced proteins in growing cells and their secretome. This method will be useful to examine the proteome and secretome changes that are linked to the altered pe…
View article: Silica-coated LiYF<sub>4</sub>:Yb<sup>3+</sup>, Tm<sup>3+</sup> upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells
Silica-coated LiYF<sub>4</sub>:Yb<sup>3+</sup>, Tm<sup>3+</sup> upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells Open
Silica-coated lanthanide-doped upconverting nanoparticles (UCNPs) are internalized by mammalian cells. The bipyramidal UCNPs are non-toxic and elicit minor stress responses in a cell type-dependent fashion.
View article: Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1
Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1 Open
Nuclear protein trafficking requires the soluble transport factor RanBP1. The subcellular distribution of RanBP1 is dynamic, as the protein shuttles between the nucleus and cytoplasm. To date, the signaling pathways regulating RanBP1 subce…
View article: Curcumin and butyrate induce fibroblast senescence without the emergence of fibrosis biomarkers
Curcumin and butyrate induce fibroblast senescence without the emergence of fibrosis biomarkers Open
Background: Small molecules have emerged as valuable tools to modulate cellular homeostasis and the changes associated with aging. In particular, the phytochemical curcumin elicits cytoprotective effects that promote human health and longe…
View article: Additional file 2 of Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases
Additional file 2 of Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases Open
Additional file 2: Supplemental Table 5. Clinical trials relevant to VCP disease.
View article: Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells
Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells Open
Intestinal epithelial cells are critical for gastrointestinal homeostasis. However, their function declines during aging. The aging-related loss of organ performance is largely driven by the increase in senescent cells. To date, the hallma…
View article: A <i>lacZ</i> reporter with high activity in the human fungal pathogen <i>Candida albicans</i>
A <i>lacZ</i> reporter with high activity in the human fungal pathogen <i>Candida albicans</i> Open
Reporter genes are useful tools to study gene transcription in various organisms. For example, the lacZ gene encoding β-galactosidase has been extensively used as a reporter in bacteria, budding yeast, fruit fly, mouse etc. However, use of…
View article: Exploring near-infrared absorbing nanocarriers to overcome cancer drug resistance
Exploring near-infrared absorbing nanocarriers to overcome cancer drug resistance Open
One of the major obstacles of successful cancer therapy is cancer drug resistance. The unique tools and applications developed by nanomedicine provide new approaches to surmount this common limitation of current treatment regimens. Nanocar…