Sjoerd Hak
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Rapid 64Cu radiolabeling and in vivo evaluation of DSPE–NODAGA liposomes in a murine mammary tumor model Open
Understanding the in vivo behavior of nanomedicines is critical for optimizing their therapeutic efficacy and facilitating personalized treatment strategies. In the quest to develop positron emission tomography (PET) methodology for liposo…
Liposomal Nω-hydroxy-l-norarginine, a proof-of-concept: Arginase inhibitors can be incorporated in liposomes while retaining their therapeutic activity ex vivo Open
Cancer immunotherapy has evolved significantly over the last decade, with therapeutics targeting the adaptive immune system showing exciting effects in clinics. Yet, the modulation of the innate immune system, particularly the tumor-associ…
Implementation of mRNA–Lipid Nanoparticle Technology in Atlantic Salmon (Salmo salar) Open
Background: This study was conducted to investigate whether mRNA vaccine technology could be adapted for the ectothermic vertebrate Atlantic salmon (Salmo salar). Lipid nanoparticle (LNP) technology has been developed and optimized for mRN…
Implementation of the mRNA-Lipid Nanoparticle Technology in Atlantic Salmon (Salmo salar) Open
Background: The study was conducted to investigate if the mRNA vaccine technology could be adapted for the ectothermic vertebrate Atlantic salmon (Salmo salar). The lipid nanoparticle (LNP) technology has been developed and optimized for m…
Rapid and scalable detection of synthetic mRNA byproducts using polynucleotide phosphorylase and polythymidine oligonucleotides Open
Production and storage of synthetic mRNA can introduce a variety of byproducts which reduce the overall integrity and functionality of mRNA vaccines and therapeutics. mRNA integrity is therefore designated as a critical quality attribute w…
Liposomes - Human phagocytes interplay in whole blood: effect of liposome design Open
Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, in vitro studies often fail to accurately translate to the complex in vivo environment. To address this gap, we employed an ex vivo huma…
Cyclic Arginine–Glycine–Aspartate‐Decorated Lipid Nanoparticle Targeting toward Inflammatory Lesions Involves Hitchhiking with Phagocytes Open
Active‐targeting nanomedicine formulations have an intricate in vivo behavior. Nanomedicines developed to target endothelial α v β 3 ‐integrin are recently demonstrated to display extensive uptake by circulating phagocytes. These phagocyte…
Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery Open
Lipid-based nanoparticles for RNA delivery (LNP-RNA) are revolutionizing the nanomedicine field, with one approved gene therapy formulation and two approved vaccines against COVID-19, as well as multiple ongoing clinical trials. As for oth…
Tumor Targeting by α<sub>v</sub>β<sub>3</sub>-Integrin-Specific Lipid Nanoparticles Occurs <i>via</i> Phagocyte Hitchhiking Open
Although the first nanomedicine was clinically approved more than two decades ago, nanoparticles' (NP) in vivo behavior is complex and the immune system's role in their application remains elusive. At present, only passive-targeting nanofo…
Mononuclear but Not Polymorphonuclear Phagocyte Depletion Increases Circulation Times and Improves Mammary Tumor-Homing Efficiency of Donor Bone Marrow-Derived Monocytes Open
Tumor associated macrophages are an essential part of the tumor microenvironment. Consequently, bone marrow-derived monocytes (BMDMs) are continuously recruited to tumors and are therefore seen as ideal delivery vehicles with tumor-targeti…
Decision letter: Optimization-by-design of hepatotropic lipid nanoparticles targeting the sodium-taurocholate cotransporting polypeptide Open
Article Figures and data Abstract Introduction Results and discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Active targeting and specific drug del…
Integrating nanomedicine and imaging Open
Biomedical engineering and its associated disciplines play a pivotal role in improving our understanding and management of disease. Motivated by past accomplishments, such as the clinical implementation of coronary stents, pacemakers or re…
View article: Real‐Time Monitoring of Nanoparticle Formation by FRET Imaging
Real‐Time Monitoring of Nanoparticle Formation by FRET Imaging Open
Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self‐assembled nanoparticles whose formation processes have been largely overlooked. He…
View article: Labeling nanoparticles: Dye leakage and altered cellular uptake
Labeling nanoparticles: Dye leakage and altered cellular uptake Open
In vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution…