Sofia Lamas
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View article: A global survey of taxa-metabolic associations across mouse microbiome communities
A global survey of taxa-metabolic associations across mouse microbiome communities Open
Host-microbiota mutualism is rooted in the exchange of dietary and metabolic molecules. Microbial diversity broadens the metabolite pool, with each taxon contributing distinct compounds in varying proportions. In the human microbiome, high…
View article: Metformin reverses infertility in a mouse model of endometriosis: unveiling disease pathways and implications for future clinical approaches
Metformin reverses infertility in a mouse model of endometriosis: unveiling disease pathways and implications for future clinical approaches Open
These results indicate that application of metformin can alleviate oxidative stress and mitigate fibrosis in endometriosis lesions in a murine model of endometriosis, which highlights metformin's potential as a pharmacological intervention…
View article: Proper micro-environment alleviates mortality in laboratory mouse breeding induced by litter overlap and older dams
Proper micro-environment alleviates mortality in laboratory mouse breeding induced by litter overlap and older dams Open
The ongoing worldwide effort to reduce animal numbers in research often omits the issue of pre-weaning mortality in mouse breeding. A conservative estimate of 20% mortality would mean approximately 1.1 M mice die annually in the EU before …
View article: Optimized Depilation Method and Comparative Analysis of Hair Growth Cycle in Mouse Strains
Optimized Depilation Method and Comparative Analysis of Hair Growth Cycle in Mouse Strains Open
In mice, hair growth follows a mosaic or wavy patterning. Therefore, synchronization of the hair growth cycle is required to adequately evaluate any trichogenic interventions pre-clinically. Depilation is the established method for synchro…
View article: Monensin as potential drug for treatment of SLeX-positive tumors
Monensin as potential drug for treatment of SLeX-positive tumors Open
Colorectal (CRC) and gastric (GC) cancers remain the top lethal cancers and targeted therapies in this setting are still very limited. Sialyl Lewis X (SLeX), a cancer-associated glycan highly expressed in both CRC and GC, plays a crucial r…
View article: Nanoparticles targeting Sialyl-Tn for efficient tyrosine kinase inhibitor delivery in gastric cancer
Nanoparticles targeting Sialyl-Tn for efficient tyrosine kinase inhibitor delivery in gastric cancer Open
Gastric cancer (GC) is the fourth leading cause of cancer-related deaths worldwide and, therefore, it is urgent to develop new and more efficient therapeutic approaches. Foretinib (FRT) is an oral multikinase inhibitor targeting MET (hepat…
View article: Protocol for infecting and monitoring susceptible k18-hACE2 mice with SARS-CoV-2
Protocol for infecting and monitoring susceptible k18-hACE2 mice with SARS-CoV-2 Open
Intranasal infection of k18-hACE2 mice with SARS-CoV-2 recapitulates the clinical characteristics present in severe COVID-19. Here, we present a protocol for intranasal administration of SARS-CoV-2 to k18-hACE2 mice and their subsequent da…
View article: SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice
SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice Open
View article: Nanoparticles Targeting Sialyl-Tn for Tyrosine Kinase Inhibitor Efficient Delivery in Gastric Cancer
Nanoparticles Targeting Sialyl-Tn for Tyrosine Kinase Inhibitor Efficient Delivery in Gastric Cancer Open
View article: The assessment of researchers’ competence in experimental procedures with laboratory animals: A three-step methodology to develop a global rating scale
The assessment of researchers’ competence in experimental procedures with laboratory animals: A three-step methodology to develop a global rating scale Open
To conduct animal experiments, researchers must be competent to handle and perform interventions on living animals in compliance with regulations. Laboratory animal science training programmes and licensing bodies therefore need to be able…
View article: Global rating scales to assess the competence of researchers in handling and performing substance administrations in laboratory rodents
Global rating scales to assess the competence of researchers in handling and performing substance administrations in laboratory rodents Open
View article: Necropsy protocol for newborn mice
Necropsy protocol for newborn mice Open
Neonatal mortality is high in laboratory mouse breeding, and causes are poorly understood. Post-mortem analysis of pups is an often overlooked source of information and insight. We present a necropsy protocol for neonatal mice designed for…
View article: C57BL/6J and B6129F1 Embryo Transfer: Unilateral and Bilateral Transfer, Embryo Number and Recipient Female Background Control for the Optimization of Embryo Survival and Litter Size
C57BL/6J and B6129F1 Embryo Transfer: Unilateral and Bilateral Transfer, Embryo Number and Recipient Female Background Control for the Optimization of Embryo Survival and Litter Size Open
Embryo transfer (ET) is a common procedure in rodent facilities. Optimizing this technique may help to reduce the number of animals, but little information is available regarding wild type strains and the conditions that affect embryo tran…
View article: Causes of death in newborn C57BL/6J mice
Causes of death in newborn C57BL/6J mice Open
Neonatal mortality in wild-type laboratory mice is an overlooked welfare and financial problem in animal facilities around the world. Causes of death are often not reported and its causes remain unknown. In this study, 324 newborn pups fro…
View article: Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains
Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains Open
Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo devel…
View article: Systemic Delivery of Bone Marrow Mesenchymal Stem Cells for In Situ Intervertebral Disc Regeneration
Systemic Delivery of Bone Marrow Mesenchymal Stem Cells for In Situ Intervertebral Disc Regeneration Open
Cell therapies for intervertebral disc (IVD) regeneration presently rely on transplantation of IVD cells or stem cells directly to the lesion site. Still, the harsh IVD environment, with low irrigation and high mechanical stress, challenge…
View article: Joint analysis of IVD herniation and degeneration by rat caudal needle puncture model
Joint analysis of IVD herniation and degeneration by rat caudal needle puncture model Open
Intervertebral disc (IVD) degeneration is responsible for various spine pathologies and present clinical treatments are insufficient. Concurrently, the mechanisms behind IVD degeneration are still not completely understood, so as to allow …