Explanipedia

Bone morphogenetic protein-2-independent bone formation by human iPSC-derived megakaryocytes and platelets Open

Norichika Mizuki, Michiaki Mukai, Yasuhiro Shiga, Naoya Takayama, Sou Nakamura , et al. · 2025

Bone regeneration (BR) is an ongoing challenge in biomedical research. While recombinant human bone morphogenetic protein-2 (rhBMP-2) is available for clinical use, its side effects remain a concern. Patient-derived platelet-rich plasma ha…

Turbulence orchestrates actin-mitochondria dynamics to preserve GPIbα and support iPSC-derived platelet biogenesis Open

Emiri Nakamura, Yasuo Harada, Sou Nakamura, Kosuke Fujio, Koenraad De Wispelaere , et al. · 2025

The in vitro manufacturing of induced pluripotent stem cell-derived platelets (iPSC-platelets) remains limited by low yield and poor quality, thus impeding practical clinical application. To address this, we developed a turbulence-assisted…

Defective flow space limits the scaling up of turbulence bioreactors for platelet generation Open

Haruki Okamoto, Kosuke Fujio, Sou Nakamura, Yasuo Harada, Hideki Hayashi , et al. · 2024

Osteogenic effects and safety of human induced pluripotent stem cell-derived megakaryocytes and platelets produced on a clinical scale Open

Takahito Arai, Yasuhiro Shiga, Michiaki Mukai, Naoya Takayama, Susumu Tashiro , et al. · 2024

A let-7 microRNA-RALB axis links the immune properties of iPSC-derived megakaryocytes with platelet producibility Open

Si Jing Chen, Kazuya Hashimoto, Kosuke Fujio, Karin Hayashi, Sudip Kumar Paul , et al. · 2024

We recently achieved the first-in-human transfusion of induced pluripotent stem cell-derived platelets (iPSC-PLTs) as an alternative to standard transfusions, which are dependent on donors and therefore variable in supply. However, heterog…

Multi-Target Regression Via Target Combinations Using Pca with Application to Quality Prediction in Oled Manufacturing Process Open

Takafumi Yamaguchi, Sou Nakamura, Yuta Yamaguchi, Yoshiyuki Yamashita · 2024

Production and nonclinical evaluation of an autologous iPSC–derived platelet product for the iPLAT1 clinical trial Open

Naoshi Sugimoto, Sou Nakamura, Shin Shimizu, Akiko Shigemasa, Junya Kanda , et al. · 2022

Donor-derived platelets are used to treat or prevent hemorrhage in patients with thrombocytopenia. However, ∼5% or more of these patients are complicated with alloimmune platelet transfusion refractoriness (allo-PTR) due to alloantibodies …

A novel coculture system for assessing respiratory sensitizing potential by IL-4 in T cells Open

Izuru Mizoguchi, Yasuhiro Katahira, Shinya Inoué, Eri Sakamoto, Aruma Watanabe , et al. · 2022

Although several in vitro assays that predict the sensitizing potential of chemicals have been developed, none can distinguish between chemical respiratory and skin sensitizers. Recently, we established a new three-dimensional dendritic ce…

Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs Open

Masamitsu Sone, Sou Nakamura, Sachiko Umeda, Harumi Ginya, Motohiko Oshima , et al. · 2021

Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production Open

H Kumon, Shinya Sakuma, Sou Nakamura, Hisataka Maruyama, Koji Eto , et al. · 2021

We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography…

Extracellular laminin regulates hematopoietic potential of pluripotent stem cells through integrin β1-ILK-β-catenin-JUN axis Open

Akinori Yuzuriha, Sou Nakamura, Naoshi Sugimoto, S. Kihara, Masato Nakagawa , et al. · 2021

Development of platelet replacement therapy using human induced pluripotent stem cells Open

Sou Nakamura, Naoshi Sugimoto, Koji Eto · 2021

In the body, platelets mainly work as a hemostatic agent, and the lack of platelets can cause serious bleeding. Induced pluripotent stem (iPS) cells potentially allow for a stable supply of platelets that are independent of donors and elim…

Ex vivo generation of platelet products from human iPS cells Open

Sou Nakamura, Naoshi Sugimoto, Koji Eto · 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity Open

Daisuke Suzuki, Charlotte Flahou, Norihide Yoshikawa, Ieva Stirblytė, Yoshikazu Hayashi , et al. · 2019

The ex vivo production of platelets depleted of human leukocyte antigen class I (HLA-I) could serve as a universal measure to overcome platelet transfusion refractoriness caused by HLA-I incompatibility. Here, we developed human induced pl…

Suppressive effects of anagrelide on cell cycle progression and the maturation of megakaryocyte progenitor cell lines in human induced pluripotent stem cells Open

Koji Takaishi, Masahiro Takeuchi, Shokichi Tsukamoto, Naoya Takayama, Motohiko Oshima , et al. · 2019

De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome Open

Tsutomu Toki, Kenichi Yoshida, RuNan Wang, Sou Nakamura, Takanobu Maekawa , et al. · 2018

Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production Open

Yukitaka Ito, Sou Nakamura, Naoshi Sugimoto, Tomohiro Shigemori, Yoshikazu Kato , et al. · 2018

Skewed megakaryopoiesis in human induced pluripotent stem cell‐derived haematopoietic progenitor cells harbouring calreticulin mutations Open

Hiraku Takei, Yoko Edahiro, Shuichi Mano, Nami Masubuchi, Yoshihisa Mizukami , et al. · 2018

Summary Somatic mutations in the calreticulin ( CALR ) gene have been found in most patients with JAK 2 ‐ and MPL ‐unmutated Philadelphia chromosome‐negative myeloproliferative neoplasms ( MPN s). It has recently been shown that mutant CAL…

Issue Information Open

Anthony Atala, Paolo De Coppi, Geoffrey C. Gurtner, Douglas W. Losordo, Alan Trounson , et al. · 2017

A representative photomicrograph showing the cell morphology and pro-platelet-forming (left inset) and a Wright-Giemsa staining (right inset) of differentiated megakaryocytes via two-stage culture system.See Guan et al. beginning on page 8…

Novel TPO receptor agonist TA-316 contributes to platelet biogenesis from human iPS cells Open

Ayako Aihara, Tomo Koike, Natsuki Abe, Sou Nakamura, Akira Sawaguchi , et al. · 2017

Key Points TA-316, a novel CMA, enhances human platelet ex vivo generation from iPS cells. TA-316 exhibited biased megakaryopoiesis at levels comparable to rhTPO and superior to eltrombopag.

A design strategy of a bioreactor for platelet generation Open

H Kumon, Shinya Sakuma, Keitaro Ito, Sou Nakamura, Koji Eto , et al. · 2017

In this study, we showed a design strategy of a bioreactor to produce platelets (PLTs) from megakaryocytes (MKs) using microfluidic chip. PLTs are generated from MKs by applying fluid force. In conventional study, slit structures have prop…

Selective Inhibition of ADAM17 Efficiently Mediates Glycoprotein Ibα Retention During Ex Vivo Generation of Human Induced Pluripotent Stem Cell-Derived Platelets Open

Shinji Hirata, Tatsunori Murata, Daisuke Suzuki, Sou Nakamura, Ryoko Jono-Ohnishi , et al. · 2016

Donor-independent platelet concentrates for transfusion can be produced in vitro from induced pluripotent stem cells (iPSCs). However, culture at 37°C induces ectodomain shedding on platelets of glycoprotein Ibα (GPIbα), the von Willebrand…

1P1-M04 On-chip monitoring of the megakaryocyte in shear flow environment Open

Kazuya Tashiro, Shinya Sakuma, Keitaro ITO, Takuya KARIWA, Sou Nakamura , et al. · 2015

Platelet transfusions are expected to be short in the future due to the donor decrease and its 4-days short storage life. Therefore it is necessary to show the process of the production of platelets from megakaryocyte (MKs) in order to pro…

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