Sripriya Ravindra Kumar
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View article: Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo Open
Cell-type-specific regulatory elements such as enhancers can direct expression of recombinant adeno-associated viruses (AAVs) to specific cell types, but this approach is limited by the relatively small packaging capacity of AAVs. In this …
View article: Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo
Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo Open
Integrating cell type-specific regulatory elements (e.g. enhancers) with recombinant adeno-associated viruses (AAVs) can provide broad and efficient genetic access to specific cell types. However, the packaging capacity of AAVs restricts t…
View article: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates Open
View article: AAV DNA library generation v1
AAV DNA library generation v1 Open
This protocol describes how to generate a DNA library of AAV capsid variants diversified by insertion of a randomized sequence encoding 7 amino acids between AA588 and AA589 of AAV9.
View article: Cloning individual AAV capsid variants v1
Cloning individual AAV capsid variants v1 Open
This protocol describes the procedure to clone individual AAV capsid variants for single variant characterization.
View article: Library tissue handling, viral DNA extraction, and NGS sample preparation v1
Library tissue handling, viral DNA extraction, and NGS sample preparation v1 Open
This protocol describes the procedure to isolate viral DNA from AAV-transfected tissue and prepare it for next-generation sequencing.
View article: Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors
Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors Open
The blood-brain barrier (BBB) presents a major challenge for delivering large molecules to study and treat the central nervous system. This is due in part to the scarcity of targets known to mediate BBB crossing. To identify novel targets,…
View article: Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier
Primate-conserved Carbonic Anhydrase IV and murine-restricted Ly6c1 are new targets for crossing the blood-brain barrier Open
The blood-brain barrier (BBB) presents a major challenge to delivering large molecules to study and treat the central nervous system (CNS). This is due in part to the scarcity of effective targets for BBB crossing, the identification of wh…
View article: Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates Open
Delivering genes to and across the brain vasculature efficiently and specifically across species remains a critical challenge for addressing neurological diseases. We have evolved adeno-associated virus (AAV9) capsids into vectors that tra…
View article: Multimodal imaging of capsid and cargo reveals differential brain targeting and liver detargeting of systemically-administered AAVs
Multimodal imaging of capsid and cargo reveals differential brain targeting and liver detargeting of systemically-administered AAVs Open
View article: Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice
Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice Open
Systemic delivery of adeno-associated virus (AAV) vectors transduces the enteric nervous system. However, less is known on the mapping and morphological and neurochemical characterization in the adult mouse colon. We used AAV9-CAG-GFP (AAV…
View article: Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems
Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems Open
Gene therapy offers great promise in addressing neuropathologies associated with the central and peripheral nervous systems (CNS and PNS). However, genetic access remains difficult, reflecting the critical need for the development of effec…
View article: Adeno-Associated Virus Toolkit to Target Diverse Brain Cells
Adeno-Associated Virus Toolkit to Target Diverse Brain Cells Open
Recombinant adeno-associated viruses (AAVs) are commonly used gene delivery vehicles for neuroscience research. They have two engineerable features: the capsid (outer protein shell) and cargo (encapsulated genome). These features can be mo…
View article: Engineered AAVs for Non-Invasive Functional Transgene Expression in Rodent and Non-Human Primate Central and Peripheral Nervous Systems
Engineered AAVs for Non-Invasive Functional Transgene Expression in Rodent and Non-Human Primate Central and Peripheral Nervous Systems Open
View article: Systemic AAV vectors for widespread and targeted gene delivery in rodents v2
Systemic AAV vectors for widespread and targeted gene delivery in rodents v2 Open
We recently developed adeno-associated virus (AAV) capsids to facilitate efficient and noninvasive gene transfer to the central and peripheral nervous systems. However, a detailed protocol for generating and systemically delivering novel A…
View article: Multicolor sparse viral labeling and 3D digital tracing of enteric plexus in mouse proximal colon using a novel adeno‐associated virus capsid
Multicolor sparse viral labeling and 3D digital tracing of enteric plexus in mouse proximal colon using a novel adeno‐associated virus capsid Open
Background Intravenous administration of adeno‐associated virus (AAV) can be used as a noninvasive approach to trace neuronal morphology and links. AAV‐PHP.S is a variant of AAV9 that effectively transduces the peripheral nervous system. T…
View article: Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types
Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types Open
View article: Systemic AAV vectors for widespread and targeted gene delivery in rodents v1
Systemic AAV vectors for widespread and targeted gene delivery in rodents v1 Open
We recently developed adeno-associated virus (AAV) capsids to facilitate efficient and noninvasive gene transfer to the central and peripheral nervous systems. However, a detailed protocol for generating and systemically delivering novel A…
View article: Publisher Correction: Systemic AAV vectors for widespread and targeted gene delivery in rodents
Publisher Correction: Systemic AAV vectors for widespread and targeted gene delivery in rodents Open
View article: Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration
Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration Open
We recently developed novel AAV capsids for efficient and noninvasive gene transfer across the central and peripheral nervous systems. In this protocol, we describe how to produce and systemically administer AAV-PHP viruses to label and/or…
View article: Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts
Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts Open
View article: Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling
Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling Open
Physiological resistance to antibiotics confounds the treatment of many chronic bacterial infections, motivating researchers to identify novel therapeutic approaches. To do this effectively, an understanding of how microbes survive in vivo…
View article: 252. Using Cre-Dependent In Vivo Selection to Identify AAV Variants That Enable Efficient and Widespread Gene Transfer to the Adult Central Nervous System
252. Using Cre-Dependent In Vivo Selection to Identify AAV Variants That Enable Efficient and Widespread Gene Transfer to the Adult Central Nervous System Open
Recombinant adeno-associated viruses (rAAVs) are commonly
\nused vehicles for in vivo gene transfer. However, the tropism
\nrepertoire of naturally occurring AAVs is limited, prompting the
\ndevelopment of novel AAV capsids with more desir…
View article: Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain Open
View article: Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo
Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo Open