Sruti Rayaprolu
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View article: Development and Clinical Validation of Blood-Based Multibiomarker Models for the Evaluation of Brain Amyloid Pathology
Development and Clinical Validation of Blood-Based Multibiomarker Models for the Evaluation of Brain Amyloid Pathology Open
Combining plasma biomarkers Aβ42/40, ptau-217, and APOE4 allele count is a scalable approach for evaluating patients with MCI for suspected AD pathology.
View article: Moderating effects of plasma glial fibrillary acidic protein along the Alzheimer's disease continuum
Moderating effects of plasma glial fibrillary acidic protein along the Alzheimer's disease continuum Open
INTRODUCTION Glial fibrillary acidic protein (GFAP) may contribute to Alzheimer's pathology at early disease stages. GFAP moderation of Alzheimer's disease (AD)‐related neurodegeneration and cognition is unclear. METHODS We examined plasma…
View article: Interrogating the plasma proteome of repetitive head impact exposure and chronic traumatic encephalopathy
Interrogating the plasma proteome of repetitive head impact exposure and chronic traumatic encephalopathy Open
View article: Development and clinical validation of blood-based multibiomarker models for the evaluation of brain amyloid pathology
Development and clinical validation of blood-based multibiomarker models for the evaluation of brain amyloid pathology Open
Background and Objectives Plasma biomarkers provide new tools to evaluate patients with mild cognitive impairment (MCI) for Alzheimer’s disease (AD) pathology. Such tools are needed for anti-amyloid therapies that require efficient and acc…
View article: Matched Proteomics of Cerebrospinal Fluid and Brain Reveals Conserved and Discordant Mechanisms of Progressive Aβ Pathology in Mice
Matched Proteomics of Cerebrospinal Fluid and Brain Reveals Conserved and Discordant Mechanisms of Progressive Aβ Pathology in Mice Open
Background Cerebrospinal fluid (CSF) is an important source of protein biomarkers for diagnosis, risk stratification, and predicting treatment response in Alzheimer's disease (AD). Proximity to brain parenchyma suggests that CSF proteomic …
View article: Age‐Dependent Changes in Perineuronal Nets and Associated Parvalbumin Interneurons in the 5xFAD Amyloidosis Mouse Model
Age‐Dependent Changes in Perineuronal Nets and Associated Parvalbumin Interneurons in the 5xFAD Amyloidosis Mouse Model Open
Background Perineuronal nets (PNNs) are extracellular matrix structures that surround neurons and proximal dendrites and regulate synaptic transmission, plasticity, and several facets of memory. PNNs are commonly associated with parvalbumi…
View article: In vivo TurboID proximity labeling to capture astrocyte proteomic and transcriptomic signatures under homeostatic and neuroinflammatory conditions
In vivo TurboID proximity labeling to capture astrocyte proteomic and transcriptomic signatures under homeostatic and neuroinflammatory conditions Open
Background Despite decades of research into the cellular and molecular contributions of brain cell types to Alzheimer’s disease (AD) pathogenesis, there are no glial‐specific disease‐modifying therapies. To address this, our group has deve…
View article: Identification of State-Specific Proteomic and Transcriptomic Signatures of Microglia-Derived Extracellular Vesicles
Identification of State-Specific Proteomic and Transcriptomic Signatures of Microglia-Derived Extracellular Vesicles Open
View article: Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles
Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles Open
Microglia are resident immune cells of the brain that play important roles in mediating inflammatory responses in several neurological diseases via direct and indirect mechanisms. One indirect mechanism may involve extracellular vesicle (E…
View article: APOE expression and secretion are modulated by mitochondrial dysfunction
APOE expression and secretion are modulated by mitochondrial dysfunction Open
Mitochondria influence cellular function through both cell-autonomous and non-cell autonomous mechanisms, such as production of paracrine and endocrine factors. Here, we demonstrate that mitochondrial regulation of the secretome is more ex…
View article: Author response: APOE expression and secretion are modulated by mitochondrial dysfunction
Author response: APOE expression and secretion are modulated by mitochondrial dysfunction Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Mitocho…
View article: Cellular Proteomic Profiling Using Proximity Labeling by TurboID-NES in Microglial and Neuronal Cell Lines
Cellular Proteomic Profiling Using Proximity Labeling by TurboID-NES in Microglial and Neuronal Cell Lines Open
Different brain cell types play distinct roles in brain development and disease. Molecular characterization of cell-specific mechanisms using cell type-specific approaches at the protein (proteomic) level can provide biological and therape…
View article: Cellular proteomic profiling using proximity labelling by TurboID-NES in microglial and neuronal cell lines
Cellular proteomic profiling using proximity labelling by TurboID-NES in microglial and neuronal cell lines Open
1.0 ABSTRACT Different brain cell types play distinct roles in brain development and disease. Molecular characterization of cell-specific mechanisms using cell type-specific approaches at the protein (proteomic) level, can provide biologic…
View article: Convergent cerebrospinal fluid proteomes and metabolic ontologies in humans and animal models of Rett syndrome
Convergent cerebrospinal fluid proteomes and metabolic ontologies in humans and animal models of Rett syndrome Open
View article: Biophysical Kv3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s
Biophysical Kv3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s Open
In Alzheimer’s disease (AD), a multitude of genetic risk factors and early biomarkers are known. Nevertheless, the causal factors responsible for initiating cognitive decline in AD remain controversial. Toxic plaques and tangles correlate …
View article: Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain
Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain Open
View article: Author response: Biophysical Kv3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s
Author response: Biophysical Kv3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract In Alzheimer's disease…
View article: APOE Expression and Secretion are Modulated by Mitochondrial Dysfunction
APOE Expression and Secretion are Modulated by Mitochondrial Dysfunction Open
Mitochondria influence cellular function through both cell-autonomous and non-cell autonomous mechanisms, such as production of paracrine and endocrine factors. Here, we demonstrate that mitochondrial regulation of the secretome is more ex…
View article: Simultaneous RNA and protein profiling using a TurboID proximity‐labeling strategy
Simultaneous RNA and protein profiling using a TurboID proximity‐labeling strategy Open
Background Biologically relevant insights into cellular disease mechanisms of neurons and glia can be obtained by complimentary molecular profiling of the transcriptome and proteome of these cells. While mutually exclusive pipelines are av…
View article: Convergent Cerebrospinal Fluid Proteomes and Metabolic Ontologies in Humans and Animal Models of Rett Syndrome
Convergent Cerebrospinal Fluid Proteomes and Metabolic Ontologies in Humans and Animal Models of Rett Syndrome Open
MECP2 loss-of-function mutations cause Rett syndrome, a disorder that results from a disrupted brain transcriptome. How these transcriptional defects are decoded into a disease proteome remains unknown. We studied the proteome in Rett synd…
View article: Cell type-specific biotin labeling <i>in vivo</i> resolves regional neuronal proteomic differences in mouse brain
Cell type-specific biotin labeling <i>in vivo</i> resolves regional neuronal proteomic differences in mouse brain Open
Isolation and proteomic profiling of brain cell types, particularly neurons, pose several technical challenges which limit our ability to resolve distinct cellular phenotypes in neurological diseases. Therefore, we generated a novel mouse …
View article: Unique molecular characteristics and microglial origin of Kv1.3 channel–positive brain myeloid cells in Alzheimer’s disease
Unique molecular characteristics and microglial origin of Kv1.3 channel–positive brain myeloid cells in Alzheimer’s disease Open
Significance The potassium channel Kv1.3 in brain myeloid cells represents a promising therapeutic target for Alzheimer’s disease (AD). The patterns of expression and functional roles of Kv1.3 channels in brain myeloid subpopulations and t…
View article: Novel proteomic molecular signatures of brain endothelial cells and microglia in the aging mouse brain
Novel proteomic molecular signatures of brain endothelial cells and microglia in the aging mouse brain Open
Background Immune and vascular mechanisms of aging and Alzheimer’s disease (AD) are mediated by microglia and endothelial cells, respectively. Transcriptomic studies of endothelial cells and microglia have revealed distinct and overlapping…
View article: Systems-based proteomics to resolve the biology of Alzheimer’s disease beyond amyloid and tau
Systems-based proteomics to resolve the biology of Alzheimer’s disease beyond amyloid and tau Open
The repeated failures of amyloid-targeting therapies have challenged our narrow understanding of Alzheimer’s disease (AD) pathogenesis and inspired wide-ranging investigations into the underlying mechanisms of disease. Increasing evidence …
View article: Flow Cytometry Approach to Characterize Phagocytic Properties of Acutely-Isolated Adult Microglia and Brain Macrophages In Vitro
Flow Cytometry Approach to Characterize Phagocytic Properties of Acutely-Isolated Adult Microglia and Brain Macrophages In Vitro Open
Microglia and central nervous system (CNS)-infiltrating macrophages, collectively called CNS mononuclear phagocytes (CNS-MPs), play central roles in neurological diseases including neurodegeneration and stroke. CNS-MPs are involved in phag…
View article: Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease
Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease Open
View article: Additional file 1 of Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease
Additional file 1 of Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease Open
Additional file 1: Table S1. Quantitative protein expression data from MACS-enriched and FACS-isolated mouse microglia. Log2 transformed protein abundance data and differential expression analysis.
View article: Additional file 2 of Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease
Additional file 2 of Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease Open
Additional file 2: Table S2. GO Elite analysis of 953 significantly differentially expressed proteins between MACS-enriched and FACS-isolated mouse microglia proteomes.
View article: Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease
Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease Open
Background Proteomic characterization of microglia provides the most proximate assessment of functionally relevant molecular mechanisms of neuroinflammation. However, microglial proteomics studies have been limited by low cellular yield an…
View article: Analysis of spinal and muscle pathology in transgenic mice overexpressing wild-type and ALS-linked mutant MATR3
Analysis of spinal and muscle pathology in transgenic mice overexpressing wild-type and ALS-linked mutant MATR3 Open
Mutations in MATR3 have been associated with amyotrophic lateral sclerosis (ALS) as well as a form of distal myopathy termed vocal cord pharyngeal distal myopathy (VCPDM). To begin to understand how mutations in MATR3 may cause disease, he…