Stefan Heckmann
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View article: A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum
A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum Open
View article: The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana
The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana Open
The synaptonemal complex (SC) forms between homologous chromosomes during meiosis. In Arabidopsis thaliana , its central region (CR) is composed of the transverse filament protein ZYP1 and the central element proteins SCEP1 and SCEP2. Here…
View article: The novel synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana
The novel synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana Open
The proteinaceous synaptonemal complex (SC) structure forms between meiotic homologous chromosomes. Its central region (CR) consists of transverse filament and central element proteins, in Arabidopsis ZYP1 and SCEP1/SCEP2, respectively. We…
View article: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: Author response: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Author response: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
View article: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: Author Response: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Author Response: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum
A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum Open
Wild plants can contribute valuable genes to their domesticated relatives. Fertility barriers and a lack of genomic resources have hindered the effective use of crop-wild introgressions. Decades of research into barley’s closest wild relat…
View article: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.)
Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.) Open
Genome editing strategies in barley ( Hordeum vulgare L. ) typically rely on Agrobacterium -mediated genetic transformation for the delivery of required genetic reagents involving tissue culture techniques. These approaches are genotype-de…
View article: Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis Open
Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underl…
View article: TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana
TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana Open
View article: High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis
High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis Open
View article: Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes
Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes Open
Nucleus, chromatin, and chromosome organization studies heavily rely on fluorescence microscopy imaging to elucidate the distribution and abundance of structural and regulatory components. Three-dimensional (3D) image stacks are a source o…
View article: The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.)
The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.) Open
Key message In barley ( Hordeum vulgare ), MTOPVIB is critical for meiotic DSB and accompanied SC and CO formation while dispensable for meiotic bipolar spindle formation. Abstract Homologous recombination during meiosis assures genetic va…
View article: Recombination Landscape Divergence Between Populations is Marked by Larger Low-Recombining Regions in Domesticated Rye
Recombination Landscape Divergence Between Populations is Marked by Larger Low-Recombining Regions in Domesticated Rye Open
The genomic landscape of recombination plays an essential role in evolution. Patterns of recombination are highly variable along chromosomes, between sexes, individuals, populations, and species. In many eukaryotes, recombination rates are…
View article: High temperature increases centromere-mediated genome elimination frequency in Arabidopsis deficient in cenH3 or its assembly factor KNL2
High temperature increases centromere-mediated genome elimination frequency in Arabidopsis deficient in cenH3 or its assembly factor KNL2 Open
Double haploid production is the most effective way of creating true-breeding lines in a single generation. In Arabidopsis , haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when outcrossed to wil…
View article: Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes
Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes Open
Nucleus, chromatin, and chromosome organization studies heavily rely on fluorescence microscopy imaging to elucidate the distribution and abundance of structural and regulatory components. Three-dimensional (3D) image stacks are a source o…
View article: High‐throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCR<sup>TM</sup>
High‐throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCR<sup>TM</sup> Open
Summary Breeding exploits novel allelic combinations assured by meiotic recombination. Barley ( Hordeum vulgare ) single pollen nucleus genotyping enables measurement of meiotic recombination rates in gametes before fertilization without t…
View article: Meiotic chromosome axis remodelling is critical for meiotic recombination in<i>Brassica rapa</i>
Meiotic chromosome axis remodelling is critical for meiotic recombination in<i>Brassica rapa</i> Open
Meiosis generates genetic variation through homologous recombination (HR) that is harnessed during breeding. HR occurs in the context of meiotic chromosome axes and the synaptonemal complex. To study the role of axis remodelling in crossov…
View article: Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering Open
View article: <i>MutS homologue 4</i> and <i>MutS homologue 5</i> Maintain the Obligate Crossover in Wheat Despite Stepwise Gene Loss following Polyploidization
<i>MutS homologue 4</i> and <i>MutS homologue 5</i> Maintain the Obligate Crossover in Wheat Despite Stepwise Gene Loss following Polyploidization Open
Crossovers (COs) ensure accurate chromosome segregation during meiosis while creating novel allelic combinations. Here, we show that allotetraploid (AABB) durum wheat (Triticum turgidum ssp. durum) utilizes two pathways of me…
View article: Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture
Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture Open
Centromeres are essential for proper chromosome segregation to the daughter cells during mitosis and meiosis. Chromosomes of most eukaryotes studied so far have regional centromeres that form primary constrictions on metaphase chromosomes.…
View article: Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy Open
In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is lar…
View article: Author response: Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
Author response: Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy Open
View article: Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy
Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy Open
In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is lar…
View article: The H3 histone chaperone NASP<sup>SIM3</sup> escorts CenH3 in Arabidopsis
The H3 histone chaperone NASP<sup>SIM3</sup> escorts CenH3 in Arabidopsis Open
Summary Centromeres define the chromosomal position where kinetochores form to link the chromosome to microtubules during mitosis and meiosis. Centromere identity is determined by incorporation of a specific histone H3 variant termed CenH3…
View article: Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in Arabidopsis thaliana
Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in Arabidopsis thaliana Open
Several histone variants are posttranslationally phosphorylated. Little is known about phosphorylation of the centromere-specific histone 3 (CENH3) variant in plants. We show that CENH3 of Arabidopsis thaliana is phosphorylated i…
View article: Variation in Recombination Rate Is Shaped by Domestication and Environmental Conditions in Barley
Variation in Recombination Rate Is Shaped by Domestication and Environmental Conditions in Barley Open
Meiotic recombination generates genetic diversity upon which selection can act. Recombination rates are highly variable between species, populations, individuals, sexes, chromosomes, and chromosomal regions. The underlying mechanisms are c…