Stephan Greiner
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View article: Whole-genome resequencing of the wild barley diversity collection: a resource for identifying and exploiting genetic variation for cultivated barley improvement
Whole-genome resequencing of the wild barley diversity collection: a resource for identifying and exploiting genetic variation for cultivated barley improvement Open
To exploit allelic variation in Hordeum vulgare subsp. spontaneum, the Wild Barley Diversity Collection was subjected to paired-end Illumina sequencing at ∼9 × depth and evaluated for several agronomic traits. We discovered 240.2 million s…
View article: Whole-Genome Sequencing of the Wild Barley Diversity Collection: A Resource for Identifying and Exploiting Genetic Variation for Cultivated Barley Improvement
Whole-Genome Sequencing of the Wild Barley Diversity Collection: A Resource for Identifying and Exploiting Genetic Variation for Cultivated Barley Improvement Open
To exploit allelic variation in Hordeum vulgare subsp. spontaneum , the Wild Barley Diversity Collection was evaluated for several agronomic traits and subjected to paired-end Illumina sequencing at ∼9X depth, generating 109.5 million sing…
View article: Graphical Abstract: Angew. Chem. Int. Ed. 35/2023
Graphical Abstract: Angew. Chem. Int. Ed. 35/2023 Open
In their Research Article (e202302003), Daniel Huster et al. analyze the dynamics of the human growth hormone secretagogue receptor.The study reveals insights into the energy landscape of the molecule.
Migration of repetitive DNAs during evolution of the permanent translocation heterozygosity in the oyster plant (Tradescantia section Rhoeo) Open
Due to translocation heterozygosity for all chromosomes in the cell complement, the oyster plant ( Tradescantia spathacea ) forms a complete meiotic ring. It also shows Rabl-arrangement at interphase, featured by polar centromere clusterin…
The evolution of multi-gene families and metabolic pathways in the evening primroses (Oenothera: Onagraceae): A comparative transcriptomics approach Open
The plant genus Oenothera has played an important role in the study of plant evolution of genomes and plant defense and reproduction. Here, we build on the 1kp transcriptomic dataset by creating 44 new transcriptomes and analyzing a total …
Graph-based models of the <i>Oenothera</i> mitochondrial genome capture the enormous complexity of higher plant mitochondrial DNA organization Open
Plant mitochondrial genomes display an enormous structural complexity, as recombining repeat-pairs lead to the generation of various sub-genomic molecules, rendering these genomes extremely challenging to assemble. We present a novel bioin…
A photosynthesis operon in the chloroplast genome drives speciation in evening primroses Open
Genetic incompatibility between the cytoplasm and the nucleus is thought to be a major factor in species formation, but mechanistic understanding of this process is poor. In evening primroses (Oenothera spp.), a model plant for organelle g…
Correction of frameshift mutations in the <i>atpB</i> gene by translational recoding in chloroplasts of <i>Oenothera</i> and tobacco Open
Translational recoding, also known as ribosomal frameshifting, is a process that causes ribosome slippage along the messenger RNA, thereby changing the amino acid sequence of the synthesized protein. Whether the chloroplast employs recodin…
Correction of frameshift mutations in the <i>atpB</i> gene by translational recoding in chloroplasts of <i>Oenothera</i> and tobacco Open
Translational recoding, also known as ribosomal frameshifting, is a process that causes ribosome slippage along the messenger RNA, thereby changing the amino acid sequence of the synthesized protein. Whether the chloroplast employs recodin…
A photosynthesis operon in the chloroplast genome drives speciation in evening primroses Open
Incompatibility between the cytoplasm and the nucleus is considered as major factor in species formation, but mechanistic understanding is poor. In evening primroses, a model plant for organelle genetics and population biology, hybrid offs…
Chloroplast nucleoids are highly dynamic in ploidy, number, and structure during angiosperm leaf development Open
Summary Chloroplast nucleoids are large, compact nucleoprotein structures containing multiple copies of the plastid genome. Studies on structural and quantitative changes of plastid DNA (ptDNA) during leaf development are scarce and have p…
Hybridization and a loss of sex shape genome‐wide diversity and the origin of species in the evening primroses (<i>Oenothera</i>, Onagraceae) Open
Summary Hybridization is thought to promote speciation in at least two ways – by fixation of heterozygosity from diploid progenitors in allopolyploids, and by generation of transgressive phenotypes and shifting fitness optima during homopl…
RNA Isolation from Plant Tissue v1 Open
Methods for RNA isolation These methods were originally included in Appendix S1 of "Evaluating Methods for Isolating Total RNA and Predicting the Success of Sequencing Phylogenetically Diverse Plant Transcriptomes" Marc T. J. Johnson et al…
The effects of losing sex on the molecular evolution of plant defense Open
It is hypothesized that the loss of sexual reproduction and reduced recombination rates decrease the ability for hosts to evolve in response to selection by parasites. Using transcriptomes from 32 species, we test whether repeated losses o…
RNA Isolation from Plant Tissue Protocol 6: pBIOZOL and Qiagen RNeasy Plant Mini Kit Method v1 Open
This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6) and was originally…
OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes Open
Organellar (plastid and mitochondrial) genomes play an important role in resolving phylogenetic relationships, and next-generation sequencing technologies have led to a burst in their availability. The ongoing massive sequencing efforts re…
Chloroplast competition is controlled by lipid biosynthesis in evening primroses Open
In most eukaryotes, organellar genomes are transmitted preferentially by the mother, but molecular mechanisms and evolutionary forces underlying this fundamental biological principle are far from understood. It is believed that biparental …
OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes Open
Key points OGDRAW has become the standard tool for displaying maps of organellar genomes it converts GenBank entries into graphical maps a new version with improved functionality has been released Abstract Organellar (plastid and mitochond…
GB2sequin - A file converter preparing custom GenBank files for database submission Open
The typical wet lab user often annotates smaller sequences in the GenBank format, but resulting files are not accepted for database submission by NCBI. This makes submission of such annotations a cumbersome task. Here we present "GB2sequin…
Thermal plasticity of the circadian clock is under nuclear and cytoplasmic control in wild barley Open
Temperature compensation, expressed as the ability to maintain clock characteristics (mainly period) in face of temperature changes, is considered a key feature of circadian clock systems. In this study, we explore the genetic basis for ci…
GenBank 2 Sequin - a file converter preparing custom GenBank files for database submission Open
The typical wet lab user often annotates smaller sequences in the GenBank format, but resulting files are not accepted for database submission by NCBI. This makes submission of such annotations a cumbersome task. Here we present “GenBank 2…
Spontaneous Chloroplast Mutants Mostly Occur by Replication Slippage and Show a Biased Pattern in the Plastome of <i>Oenothera</i> Open
Spontaneous plastome mutants have been used as a research tool since the beginning of genetics. However, technical restrictions have severely limited their contributions to research in physiology and molecular biology. Here, we used full p…