Stephan Harm
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View article: Does CytoSorb Interfere with Immunosuppression? A Pharmacokinetic and Functional Evaluation
Does CytoSorb Interfere with Immunosuppression? A Pharmacokinetic and Functional Evaluation Open
Background/Objectives: Cytokine release during organ transplantation contributes to primary graft dysfunction and requires careful immunomodulation. CytoSorb, a hemoadsorption device developed to reduce circulating cytokine levels, is incr…
View article: Influence of heparin-based anticoagulants on antibiotic therapy
Influence of heparin-based anticoagulants on antibiotic therapy Open
Background A world without antibiotics is hard to conceive. They have revolutionized the treatment landscape for bacterial infections, reducing mortality rates and enabling complex medical procedures. However, their widespread use has fuel…
View article: Endpoint attachment of unfractionated vs low molecular weight heparin: a comparative study on blood compatibility
Endpoint attachment of unfractionated vs low molecular weight heparin: a comparative study on blood compatibility Open
Introduction Blood compatibility is a critical requirement for materials used in medical devices to prevent adverse reactions such as thrombosis and inflammation. Heparin, a natural anticoagulant, is commonly used to coat medical devices s…
View article: The neutralizing effect of heparin on blood-derived antimicrobial compounds: impact on antibacterial activity and inflammatory response
The neutralizing effect of heparin on blood-derived antimicrobial compounds: impact on antibacterial activity and inflammatory response Open
Acting through a combination of direct and indirect pathogen clearance mechanisms, blood-derived antimicrobial compounds (AMCs) play a pivotal role in innate immunity, safeguarding the host against invading microorganisms. Besides their an…
View article: Heparin enables the reliable detection of endotoxin in human blood samples using the Limulus amebocyte lysate assay
Heparin enables the reliable detection of endotoxin in human blood samples using the Limulus amebocyte lysate assay Open
The determination of lipopolysaccharide (endotoxin) in serum or plasma samples using Limulus amebocyte lysate (LAL)-based assays is currently not sufficiently reliable in clinical diagnostics due to numerous interfering factors that strong…
View article: An in vitro study on factors affecting endotoxin neutralization in human plasma using the Limulus amebocyte lysate test
An in vitro study on factors affecting endotoxin neutralization in human plasma using the Limulus amebocyte lysate test Open
Endotoxin neutralization, caused by plasma components, makes it difficult to detect endotoxins in human blood. In this study, we investigated which factors influence the recovery of endotoxins using limulus ameobocyte lysate (LAL)-based as…
View article: Impact of anion exchange adsorbents on regional citrate anticoagulation
Impact of anion exchange adsorbents on regional citrate anticoagulation Open
Introduction: Heparin and citrate are commonly used anticoagulants in membrane/adsorption based extracorporeal liver support systems. However, anion exchange resins employed for the removal of negatively charged target molecules including …
View article: Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin
Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin Open
This ex vivo proof-of-concept study aimed to investigate the effect of nanosilver particles (AgNP) added to a conventional infiltrant resin (Icon) on external penetration into natural proximal enamel caries exceeding into dentin after inte…
View article: Blood Compatibility—An Important but Often Forgotten Aspect of the Characterization of Antimicrobial Peptides for Clinical Application
Blood Compatibility—An Important but Often Forgotten Aspect of the Characterization of Antimicrobial Peptides for Clinical Application Open
Acylation of antimicrobial peptides mimics the structure of the natural lipopeptide polymyxin B, and increases antimicrobial and endotoxin-neutralizing activities. In this study, the antimicrobial properties of lactoferrin-based LF11 pepti…
Influence of citrate concentration on the activation of blood cells in an in vitro dialysis setup Open
Our data indicate that an increase in the citrate concentration from 3 mM to 4 mM further dampens cellular activation, thereby improving biocompatibility. A concentration of 4 mM citrate might therefore be optimal for use in clinical pract…
View article: Removal of stabilizers from human serum albumin by adsorbents and dialysis used in blood purification
Removal of stabilizers from human serum albumin by adsorbents and dialysis used in blood purification Open
The outcome of this study shows that activated charcoal based adsorbents are more effective in removal of N-acetylthryptophanate, whereas polystyrene based adsorbents are better for the removal of caprylate from HSA solutions. An adsorbent…
View article: Supplementary Material for: Clearance of Selected Plasma Cytokines with Continuous Veno-Venous Hemodialysis Using Ultraflux EMiC2 versus Ultraflux AV1000S
Supplementary Material for: Clearance of Selected Plasma Cytokines with Continuous Veno-Venous Hemodialysis Using Ultraflux EMiC2 versus Ultraflux AV1000S Open
Background: High cutoff hemofilters might support the restoration of immune homeostasis in systemic inflammation by depleting inflammatory mediators from the circulation. Methods: Interleukin (IL)-6, IL-8, IL-10, and tumor necrosis factor-…
View article: Low-dose polymyxin: an option for therapy of Gram-negative sepsis
Low-dose polymyxin: an option for therapy of Gram-negative sepsis Open
Endotoxins are the major components of the outer membrane of most Gram-negative bacteria and are one of the main targets in inflammatory diseases. The presence of endotoxins in blood can provoke septic shock in case of pronounced immune re…