Stéphanie Flament
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View article: Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody
Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody Open
Monoclonal antibodies recognizing nonprotein antigens remain largely underrepresented in our understanding of the molecular repertoire of innate and adaptive immunity. One such antibody is Mannitou, a murine IgM that recognizes paucimannos…
View article: Analysis of Unfolded Protein Response Activation in Colon Adenocarcinoma Epithelial Cells: A Proteomic Study
Analysis of Unfolded Protein Response Activation in Colon Adenocarcinoma Epithelial Cells: A Proteomic Study Open
Purpose High throughput technologies have identified molecular patterns in colorectal cancer (CRC) cells, aiding in modeling responses to anti‐cancer treatments. The different responses observed depend on the type of cancer, the tumour gra…
View article: Comparative Analysis of Laboratory-Scale Immunoglobulin G Purification Methods from Human Serum
Comparative Analysis of Laboratory-Scale Immunoglobulin G Purification Methods from Human Serum Open
Immunoglobulin G (IgG) purification is a critical process for evaluating its role in autoimmune diseases, which are defined by the occurrence of autoantibodies. Affinity chromatography with protein G is widely considered to be the optimal …
View article: Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics
Low-Invasive Sampling Method with Tape-Disc Sampling for the Taxonomic Identification of Archeological and Paleontological Bones by Proteomics Open
Collagen from paleontological bones is an important organic material for isotopic measurement, radiocarbon analysis, and paleoproteomic analysis to provide information on diet, dating, taxonomy, and phylogeny. Current paleoproteomic method…
View article: Low-invasive sampling method for taxonomic for the identification of archaeological and paleontological bones by proteomics of their collagens
Low-invasive sampling method for taxonomic for the identification of archaeological and paleontological bones by proteomics of their collagens Open
Collagen from paleontological bones is an important organic material for isotopic measurement, radiocarbon and paleoproteomic analyzes, to provide information on diet, dating and taxonomy. Current paleoproteomics methods are destructive an…
View article: Characterization of a bovine serum albumin model system for in vitro fortification of N ε -carboxymethyl-lysine
Characterization of a bovine serum albumin model system for in vitro fortification of N ε -carboxymethyl-lysine Open
International audience
View article: Paléoprotéomique appliquée au site de Waziers, le « Bas‑Terroir » (Nord)
Paléoprotéomique appliquée au site de Waziers, le « Bas‑Terroir » (Nord) Open
International audience
View article: The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein Open
Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bact…
View article: Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition
Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition Open
Diazotrophic bacteria isolated from the rhizosphere of a wild wheat ancestor, grown from its refuge area in the Fertile Crescent, were found to be efficient Plant Growth-Promoting Rhizobacteria (PGPR), upon interaction with an elite wheat …
View article: Reliable Approach for Pure Yeast Cell Wall Protein Isolation from <i>Saccharomyces cerevisiae</i> Yeast Cells
Reliable Approach for Pure Yeast Cell Wall Protein Isolation from <i>Saccharomyces cerevisiae</i> Yeast Cells Open
Saccharomyces cerevisiae yeast is a fungus presenting a peripheral organelle called the cell wall. The cell wall protects the yeast cell from stress and provides means for communication with the surrounding environment. It has a com…
View article: Exudates from wild wheat rhizosphere-associated PGPR: effect on root development in modern wheat and composition
Exudates from wild wheat rhizosphere-associated PGPR: effect on root development in modern wheat and composition Open
LCMS raw data from: Two diazotrophic bacteria, BPMP-PU-28 and BPMP-EL-40, isolated from the rhizosphere of a wild wheat ancestor (T. t. dicoccoides) grown in soil from its refuge area in the Fertile Crescent or f…
View article: Extinct species identification from late middle Pleistocene and earlier Upper Pleistocene bone fragments and tools not recognizable from their osteomorphological study by an enhanced proteomics protocol
Extinct species identification from late middle Pleistocene and earlier Upper Pleistocene bone fragments and tools not recognizable from their osteomorphological study by an enhanced proteomics protocol Open
Ancient preserved molecules offer the opportunity of gaining a deeper knowledge on their biological past. However, the development of a proteomic workflow remains a challenge. The analysis of fossils must involve a low quantity of material…
View article: Effect of milk heat treatment on molecular interactions during the process of Qishta, a Lebanese dairy product
Effect of milk heat treatment on molecular interactions during the process of Qishta, a Lebanese dairy product Open
View article: Mitochondrial O-GlcNAc Transferase Interacts with and Modifies Many Proteins and Its Up-Regulation Affects Mitochondrial Function and Cellular Energy Homeostasis
Mitochondrial O-GlcNAc Transferase Interacts with and Modifies Many Proteins and Its Up-Regulation Affects Mitochondrial Function and Cellular Energy Homeostasis Open
O-GlcNAcylation is a cell glucose sensor. The addition of O-GlcNAc moieties to target protein is catalyzed by the O-Linked N-acetylglucosamine transferase (OGT). OGT is encoded by a single gene that yields differentially spliced OGT isofor…
View article: Extinct species identification from Upper Pleistocene bone fragments not identifiable from their osteomorphological studies by proteomics analysis
Extinct species identification from Upper Pleistocene bone fragments not identifiable from their osteomorphological studies by proteomics analysis Open
The ancient preserved molecules offer the opportunity to gain a better knowledge on the biological past. In recent years, bones proteomics has become an attractive method to study the animal biological origin, extinct species and species e…
View article: Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology Open
Starch granule morphology is highly variable depending on the botanical origin. Moreover, all investigated plant species display intra-tissular variability of granule size. In potato tubers, the size distribution of starch granules follows…
View article: Comparaison de l’efficacité d'un traitement médical par sandostatine LAR® avant chirurgie à un traitement chirurgical sans préparation, chez des patients acromégales nouvellement diagnostiqués
Comparaison de l’efficacité d'un traitement médical par sandostatine LAR® avant chirurgie à un traitement chirurgical sans préparation, chez des patients acromégales nouvellement diagnostiqués Open
L'acromegalie est une pathologie secondaire a un adenome hypophysaire dans 98% des cas. Sa prevalence est de 40 a 70 cas par million d'habitants, son incidence de 3 a 4 cas par million d'habitants. Les adenomes somatotropes sont majoritair…