Stephen E. Braun
YOU?
Author Swipe
View article: Plasma circulating extracellular vesicles reveal dysregulation of synaptic signaling in SHIV-infected rhesus macaque
Plasma circulating extracellular vesicles reveal dysregulation of synaptic signaling in SHIV-infected rhesus macaque Open
Background Antiretroviral therapy suppresses human immunodeficiency virus (HIV) replication; however, nearly 50% of HIV infected patients suffer from HIV-associated neurocognitive disorders (HAND). Recently, we reported that proteins in pl…
View article: Clinically approved immunotoxins targeting hematological cancers: “the best of both worlds”
Clinically approved immunotoxins targeting hematological cancers: “the best of both worlds” Open
Hematological malignancies contribute significantly to the overall cancer burden. Certain subtypes, such as hairy cell leukemia (HCL), are chronic and characterized by residual disease after first-line therapy, while others, such as blasti…
View article: Regulation of Collecting Lymphatic Vessel Contractile Function by TRPV4 Channels
Regulation of Collecting Lymphatic Vessel Contractile Function by TRPV4 Channels Open
BACKGROUND: Dysregulation of TRPV4 (transient receptor potential vanilloid type 4)–mediated signaling has been associated with inflammation and tissue fibrosis, both of which are key features in the pathophysiology of lymphatic system dise…
View article: Persistence of CMV-specific anti-HIV CAR T cells after adoptive immunotherapy
Persistence of CMV-specific anti-HIV CAR T cells after adoptive immunotherapy Open
The success of chimeric antigen receptor (CAR)-T cell (Tc) immunotherapy in refractory B-cell acute lymphoblastic leukemia (B-ALL) suggests adaptation of this strategy toward HIV. Because cytomegalovirus (CMV) vaccine vectors generated Tc …
View article: P22.15.B THE DEVELOPMENT OF C-SMART: A NOVEL COGNITIVE REHABILITATION AND MINDFULNESS INTERVENTION FOR PATIENTS WITH BRAIN TUMORS
P22.15.B THE DEVELOPMENT OF C-SMART: A NOVEL COGNITIVE REHABILITATION AND MINDFULNESS INTERVENTION FOR PATIENTS WITH BRAIN TUMORS Open
BACKGROUND Patients diagnosed with brain tumors experience significant cognitive sequelae, yet they remain underrepresented in behavioral intervention research. We present the development and initial results from the pilot study of C-SMART…
View article: Extracellular domain, hinge, and transmembrane determinants affecting surface CD4 expression of a novel anti-HIV chimeric antigen receptor (CAR) construct
Extracellular domain, hinge, and transmembrane determinants affecting surface CD4 expression of a novel anti-HIV chimeric antigen receptor (CAR) construct Open
Chimeric antigen receptor (CAR)-T cells have demonstrated clinical potential, but current receptors still need improvements to be successful against chronic HIV infection. In this study, we address some requirements of CAR motifs for stron…
View article: TRPV4-Expressing Tissue-Resident Macrophages Regulate the Function of Collecting Lymphatic Vessels via Thromboxane A2 Receptors in Lymphatic Muscle Cells
TRPV4-Expressing Tissue-Resident Macrophages Regulate the Function of Collecting Lymphatic Vessels via Thromboxane A2 Receptors in Lymphatic Muscle Cells Open
Rationale TRPV4 channels are critical regulators of blood vascular function and have been shown to be dysregulated in many disease conditions in association with inflammation and tissue fibrosis. These are key features in the pathophysiolo…
View article: Extracellular domain, hinge, and transmembrane determinants affecting surface CD4 expression of a novel anti-HIV chimeric antigen receptor (CAR) construct
Extracellular domain, hinge, and transmembrane determinants affecting surface CD4 expression of a novel anti-HIV chimeric antigen receptor (CAR) construct Open
Chimeric antigen receptor (CAR)-T cells have demonstrated clinical potential, but current receptors still need improvements to be successful against chronic HIV infection. In this study, we address some requirements of CAR motifs for stron…
View article: SMRT Sequencing Enables High-Throughput Identification of Novel AAVs from Capsid Shuffling and Directed Evolution
SMRT Sequencing Enables High-Throughput Identification of Novel AAVs from Capsid Shuffling and Directed Evolution Open
The use of AAV capsid libraries coupled with various selection strategies has proven to be a remarkable approach for generating novel AAVs with enhanced and desired features. The inability to reliably sequence the complete capsid gene in a…
View article: Circulating Plasma Exosomal Proteins of Either SHIV-Infected Rhesus Macaque or HIV-Infected Patient Indicates a Link to Neuropathogenesis
Circulating Plasma Exosomal Proteins of Either SHIV-Infected Rhesus Macaque or HIV-Infected Patient Indicates a Link to Neuropathogenesis Open
Despite the suppression of human immunodeficiency virus (HIV) replication by combined antiretroviral therapy (cART), 50–60% of HIV-infected patients suffer from HIV-associated neurocognitive disorders (HAND). Studies are uncovering the rol…
View article: Targeting TRAF3IP2 inhibits angiogenesis in glioblastoma
Targeting TRAF3IP2 inhibits angiogenesis in glioblastoma Open
Increased vascularization, also known as neoangiogenesis, plays a major role in many cancers, including glioblastoma multiforme (GBM), by contributing to their aggressive growth and metastasis. Although anti-angiogenic therapies provide so…
View article: Isolation and short‐term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study
Isolation and short‐term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study Open
Objective To develop an experimental method for routine isolation and short‐term culture of primary lymphatic endothelial cells from specific collecting vessels. Methods Lymphatic endothelial cell tubes (LECTs) were isolated from micro‐dis…
View article: Biallelic, Selectable, Knock-in Targeting of CCR5 via CRISPR-Cas9 Mediated Homology Directed Repair Inhibits HIV-1 Replication
Biallelic, Selectable, Knock-in Targeting of CCR5 via CRISPR-Cas9 Mediated Homology Directed Repair Inhibits HIV-1 Replication Open
Transplanting HIV-1 positive patients with hematopoietic stem cells homozygous for a 32 bp deletion in the chemokine receptor type 5 (CCR5) gene resulted in a loss of detectable HIV-1, suggesting genetically disrupting CCR5 is a promising …
View article: HIV-based lentiviral vectors: Origin and sequence differences
HIV-based lentiviral vectors: Origin and sequence differences Open
Three gene therapy strategies have received US Food and Drug Administration (FDA) approval; one includes HIV-1-based lentiviral vectors. These vectors incorporate features to provide long-term gene transfer and expression while minimizing …
View article: Increased Efficiency for Biallelic Mutations of the <i>CCR5</i> Gene by CRISPR-Cas9 Using Multiple Guide RNAs As a Novel Therapeutic Option for Human Immunodeficiency Virus
Increased Efficiency for Biallelic Mutations of the <i>CCR5</i> Gene by CRISPR-Cas9 Using Multiple Guide RNAs As a Novel Therapeutic Option for Human Immunodeficiency Virus Open
CCR5 is a coreceptor of human immunodeficiency virus type 1 (HIV-1). Transplantation of hematopoietic stem cells homozygous for a 32-bp deletion in CCR5 resulted in a loss of detectable HIV-1 in two patients, suggesting that genetic strate…
View article: Identification and Targeting of Thomsen–Friedenreich and IL1RAP Antigens on Chronic Myeloid Leukemia Stem Cells Using Bi-Specific Antibodies
Identification and Targeting of Thomsen–Friedenreich and IL1RAP Antigens on Chronic Myeloid Leukemia Stem Cells Using Bi-Specific Antibodies Open
These results show that the bi-specific antibody is capable of targeting IL1RAP+ and CD176+ cell population among CML PBMCs, but not corresponding normal cells in CDC assay. We hereby offer a novel strategy for the depletion of CML stem ce…
View article: A Novel function of Nebivolol: Stimulation of Adipose-derived Stem Cell Proliferation and Inhibition of Differentiation
A Novel function of Nebivolol: Stimulation of Adipose-derived Stem Cell Proliferation and Inhibition of Differentiation Open
Tissue engineering is limited by the time of culture expansion of cells needed for scaffold seeding. Thus, a simple means of accelerated stem cell proliferation could represent a significant advance. Here, Nebivolol was investigated for it…
View article: A New Humanized Mouse Model Mimics Humans in Lacking α-Gal Epitopes and Secreting Anti-Gal Antibodies
A New Humanized Mouse Model Mimics Humans in Lacking α-Gal Epitopes and Secreting Anti-Gal Antibodies Open
Mice have been used as accepted tools for investigating complex human diseases and new drug therapies because of their shared genetics and anatomical characteristics with humans. However, the tissues in mice are different from humans in th…
View article: Abstract 1222A: Identification and targeting of CD34+CD176+IL1RAP+ chronic myeloid leukemia stem cells with bi-specific antibodies
Abstract 1222A: Identification and targeting of CD34+CD176+IL1RAP+ chronic myeloid leukemia stem cells with bi-specific antibodies Open
Leukemia Stem Cells (LSCs) quiescence in Chronic Myeloid Leukemia (CML) plays a major role in therapeutic resistance and disease progression calling for the need for identifying and targeting such cells (1). LSCs belong to the primitive po…
View article: The Membrane-Active Phytopeptide Cycloviolacin O2 Simultaneously Targets HIV-1-infected Cells and Infectious Viral Particles to Potentiate the Efficacy of Antiretroviral Drugs
The Membrane-Active Phytopeptide Cycloviolacin O2 Simultaneously Targets HIV-1-infected Cells and Infectious Viral Particles to Potentiate the Efficacy of Antiretroviral Drugs Open
Background: Novel strategies to increase the efficacy of antiretroviral (ARV) drugs will be of crucial importance. We hypothesize that membranes of HIV-1-infected cells and enveloped HIV-1 particles may be preferentially targeted by the ph…
View article: TRAF3IP2, a novel therapeutic target in glioblastoma multiforme
TRAF3IP2, a novel therapeutic target in glioblastoma multiforme Open
Glioblastoma multiforme (glioblastoma) remains one of the deadliest cancers. Pro-inflammatory and pro-tumorigenic mediators present in tumor microenvironment (TME) facilitate communication between tumor cells and adjacent non-malignant cel…
View article: A novel model to characterize structure and function of BRCA1
A novel model to characterize structure and function of BRCA1 Open
BRCA1 plays a central role in DNA repair. Although N‐terminal RING and C‐terminal BRCT domains are studied well, the functions of the central region of BRCA1 are poorly characterized. Here, we report a structural and functional analysis of…
View article: NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-α
NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-α Open
Natriuretic peptide receptor 3 (NPR3) is a clearance receptor by binding and internalizing natriuretic peptides (NPs) for ultimate degradation. Patients with cardiac failure show elevated NPs. NPs are linked to poor long-term survival beca…
View article: Intratunical Injection of Genetically Modified Adipose Tissue-Derived Stem Cells with Human Interferon α-2b for Treatment of Erectile Dysfunction in a Rat Model of Tunica Albugineal Fibrosis
Intratunical Injection of Genetically Modified Adipose Tissue-Derived Stem Cells with Human Interferon α-2b for Treatment of Erectile Dysfunction in a Rat Model of Tunica Albugineal Fibrosis Open
Introduction Peyronie's disease (PD) has frequently been associated with erectile dysfunction (ED) and may further compromise coitus. Aim To investigate the efficacy of intratunical injection of genetically modified rat adipose tissue-deri…
View article: 459. CAR-Transduced T Cells Demonstrate Increased CTL Function
459. CAR-Transduced T Cells Demonstrate Increased CTL Function Open
Infection with HIV-1 results in CD4+ T cell depletion and the subsequent loss of immune function results in AIDS. Although HAART lowers plasma viremia, it requires life-long drug therapy. However, elite controllers are able to exert contro…