Stephen L. Rogers
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View article: Organ-specific rewiring of mitochondrial integrity through COX7A dictates cellular ploidy control
Organ-specific rewiring of mitochondrial integrity through COX7A dictates cellular ploidy control Open
To achieve proper cell and tissue size, cytoplasmic and nuclear growth must be coordinated. Disrupting this coordination causes birth defects and disease. In nature’s largest cells, nuclear growth occurs through polyploidization (whole-gen…
View article: EB-SUN, a New Microtubule Plus-End Tracking Protein in Drosophila.
EB-SUN, a New Microtubule Plus-End Tracking Protein in Drosophila. Open
Microtubule (MT) regulation is essential for oocyte development. In
View article: EB-SUN, a new microtubule plus-end tracking protein in <i>Drosophila</i>
EB-SUN, a new microtubule plus-end tracking protein in <i>Drosophila</i> Open
Microtubule (MT) regulation is essential for oocyte development. In Drosophila, MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated …
View article: <scp>CryoET</scp> shows cofilactin filaments inside the microtubule lumen
<span>CryoET</span> shows cofilactin filaments inside the microtubule lumen Open
Cytoplasmic microtubules are tubular polymers that can harbor small proteins or filaments inside their lumen. The identities of these objects and mechanisms for their accumulation have not been conclusively established. Here, we used cryog…
View article: CryoET shows cofilactin filaments inside the microtubule lumen
CryoET shows cofilactin filaments inside the microtubule lumen Open
Cytoplasmic microtubules are tubular polymers that can harbor small proteins or filaments inside their lumen. The identity of these objects and what causes their accumulation has not been conclusively established. Here, we used cryogenic e…
View article: Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation
Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation Open
Endothelial cells line all blood vessels, where they coordinate blood vessel formation and the blood-tissue barrier via regulation of cell-cell junctions. The nucleus also regulates endothelial cell behaviors, but it is unclear how the nuc…
View article: Author response: Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation
Author response: Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstract Endothelial…
View article: Nuclear SUN1 Stabilizes Endothelial Cell Junctions via Microtubules to Regulate Blood Vessel Formation
Nuclear SUN1 Stabilizes Endothelial Cell Junctions via Microtubules to Regulate Blood Vessel Formation Open
Endothelial cells line all blood vessels, where they coordinate blood vessel formation and the blood-tissue barrier via regulation of cell-cell junctions. The nucleus also regulates endothelial cell behaviors, but it is unclear how the nuc…
View article: Spindle microtubules in flux
Spindle microtubules in flux Open
Accurate and timely chromosome segregation is a task performed within meiotic and mitotic cells by a specialized force-generating structure - the spindle. This micromachine is constructed from numerous proteins, most notably the filamentou…
View article: Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment
Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment Open
Glia play crucial roles in ensheathing axons, a process that requires an intricate series of glia-neuron interactions. The membrane-anchored protein Wrapper is present in Drosophila midline glia and is required for ensheathment of commissu…
View article: Enabled plays key roles in embryonic epithelial morphogenesis in Drosophila
Enabled plays key roles in embryonic epithelial morphogenesis in Drosophila Open
Studies in cultured cells and in vitro have identified many actin regulators and begun to define their mechanisms of action. Among these are Enabled (Ena)/VASP proteins, anti-Capping proteins that influence fibroblast migration, growth con…
View article: The actin-microtubule cross-linking activity of Drosophila Short stop is regulated by intramolecular inhibition
The actin-microtubule cross-linking activity of Drosophila Short stop is regulated by intramolecular inhibition Open
Actin and microtubule dynamics must be precisely coordinated during cell migration, mitosis, and morphogenesis—much of this coordination is mediated by proteins that physically bridge the two cytoskeletal networks. We have investigated the…
View article: Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.
Serum-free adapted Drosophila S2R+ line is amenable to RNA interference. Open
Drosophila Schneider S2 cell lines are susceptible to RNA interference (RNAi) and this attribute has cemented Drosophila cell lines as an important tool for high throughput functional genomics screening (Rogers and Rogers 2008; Zhou et al.…
View article: The SCF Slimb ubiquitin ligase regulates Plk4/Sak levels to block centriole reduplication
The SCF Slimb ubiquitin ligase regulates Plk4/Sak levels to block centriole reduplication Open
Restricting centriole duplication to once per cell cycle is critical for chromosome segregation and genomic stability, but the mechanisms underlying this block to reduplication are unclear. Genetic analyses have suggested an involvement fo…
View article: Excess centrosomes disrupt endothelial cell migration via centrosome scattering
Excess centrosomes disrupt endothelial cell migration via centrosome scattering Open
Centrosome–microtubule interactions during interphase are important for centrosome clustering and cell polarity.Supernumerary centrosomes contribute to spindle defects and aneuploidy at mitosis, but the effects of excess centrosomes during…
View article: Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis
Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis Open
G-protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G-protein α subunit (EhGα1) signaling from Enta…
View article: Expression Levels of a Kinesin-13 Microtubule Depolymerase Modulates the Effectiveness of Anti-Microtubule Agents
Expression Levels of a Kinesin-13 Microtubule Depolymerase Modulates the Effectiveness of Anti-Microtubule Agents Open
BackgroundChemotheraputic drugs often target the microtubule cytoskeleton as a means to disrupt cancer cell mitosis and proliferation. Anti-microtubule drugs inhibit microtubule dynamics, thereby triggering apoptosis when dividing cells ac…
View article: The Non-Catalytic Domains of Drosophila Katanin Regulate Its Abundance and Microtubule-Disassembly Activity
The Non-Catalytic Domains of Drosophila Katanin Regulate Its Abundance and Microtubule-Disassembly Activity Open
Microtubule severing is a biochemical reaction that generates an internal break in a microtubule and regulation of microtubule severing is critical for cellular processes such as ciliogenesis, morphogenesis, and meiosis and mitosis. Katani…
View article: TOG Proteins Are Spatially Regulated by Rac-GSK3β to Control Interphase Microtubule Dynamics
TOG Proteins Are Spatially Regulated by Rac-GSK3β to Control Interphase Microtubule Dynamics Open
Microtubules are regulated by a diverse set of proteins that localize to microtubule plus ends (+TIPs) where they regulate dynamic instability and mediate interactions with the cell cortex, actin filaments, and organelles. Although individ…
View article: Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility
Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility Open
Cultured Drosophila melanogaster S2 and S2R + cell lines have become important tools for uncovering fundamental aspects of cell biology as well as for gene discovery. Despite their utility, these cell lines are nonmotile and cannot build p…
View article: The Drosophila Dead end Arf-like3 GTPase controls vesicle trafficking during tracheal fusion cell morphogenesis
The Drosophila Dead end Arf-like3 GTPase controls vesicle trafficking during tracheal fusion cell morphogenesis Open
The Drosophila larval tracheal system consists of a highly branched tubular organ that becomes interconnected by migration-fusion events during embryonic development. Fusion cells at the tip of each branch guide migration, adhere, and then…
View article: The Spectraplakin Short Stop Is an Actin-Microtubule Cross-Linker That Contributes to Organization of the Microtubule Network
The Spectraplakin Short Stop Is an Actin-Microtubule Cross-Linker That Contributes to Organization of the Microtubule Network Open
The dynamics of actin and microtubules are coordinated in a variety of cellular and morphogenetic processes; however, little is known about the molecules mediating this cytoskeletal cross-talk. We are studying Short stop (Shot), the sole D…
View article: Drosophila Ric-8 interacts with the G 12/13 subunit, Concertina, during activation of the Folded gastrulation pathway
Drosophila Ric-8 interacts with the G 12/13 subunit, Concertina, during activation of the Folded gastrulation pathway Open
Heterotrimeric G proteins, composed of α, β, and
View article: The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array Open
Structures of Drosophila Msps TOG4 and human ch-TOG TOG4 are presented. TOG4 departs from the other TOG structures and predicts novel α-tubulin engagement. Whereas TOG domains across the array have different tubulin-binding properties, cel…
View article: The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics
The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics Open
An in vivo structure–function analysis of the Drosophila homologue Mini spindles (Msps) identified novel domains that are necessary for the interplay between the conserved TOG domains and inter-TOG microtubule (MT) binding that underlies t…
View article: A Multicomponent Assembly Pathway Contributes to the Formation of Acentrosomal Microtubule Arrays in Interphase Drosophila Cells
A Multicomponent Assembly Pathway Contributes to the Formation of Acentrosomal Microtubule Arrays in Interphase Drosophila Cells Open
In animal cells, centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm. Drosophila presents an interesting paradox however, as centrosome-deficient mutant animals develop into viable adults. To understand t…
View article: The<i>Drosophila melanogaster</i>Rab GAP RN-tre cross-talks with the Rho1 signaling pathway to regulate nonmuscle myosin II localization and function
The<i>Drosophila melanogaster</i>Rab GAP RN-tre cross-talks with the Rho1 signaling pathway to regulate nonmuscle myosin II localization and function Open
The Rab GAP RN-tre regulates the activity, coalescence, and function of nonmuscle myosin II in Drosophila melanogaster cells through cross-talk with the Rho1 signaling pathway. This regulation is partially independent of RN-tre’s GAP activ…
View article: Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development
Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development Open
Axonal growth and targeting are fundamental to the organization of the nervous system, and require active engagement of the cytoskeleton. Polymerization and stabilization of axonal microtubules is central to axonal growth and maturation of…
View article: The Gas2 family protein Pigs is a microtubule +TIP that affects cytoskeleton organisation
The Gas2 family protein Pigs is a microtubule +TIP that affects cytoskeleton organisation Open
Coordination between different cytoskeletal systems is crucial for many cell biological functions, including cell migration and mitosis, and also plays an important role during tissue morphogenesis. Proteins of the class of cytoskeletal cr…
View article: Non-enzymatic Activity of the α-Tubulin Acetyltransferase αTAT Limits Synaptic Bouton Growth in Neurons
Non-enzymatic Activity of the α-Tubulin Acetyltransferase αTAT Limits Synaptic Bouton Growth in Neurons Open