Stephen Lory
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View article: Revealing the impact of Pseudomonas aeruginosa quorum sensing molecule 2’-aminoacetophenone on the human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip
Revealing the impact of Pseudomonas aeruginosa quorum sensing molecule 2’-aminoacetophenone on the human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip Open
Background Pseudomonas aeruginosa (PA) causes severe respiratory infections utilizing multiple virulence functions. Previous findings on the PA secreted quorum sensing (QS)-regulated small molecule, 2’-aminoacetophenone (2-AA), revealed it…
View article: Revealing the impact of <i>Pseudomonas aeruginosa</i> quorum sensing molecule 2’-aminoacetophenone on human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip
Revealing the impact of <i>Pseudomonas aeruginosa</i> quorum sensing molecule 2’-aminoacetophenone on human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip Open
Pseudomonas aeruginosa (PA) causes severe respiratory infections utilizing multiple virulence functions. Our previous findings on PA quorum sensing (QS)-regulated small molecule, 2’-aminoacetophenone (2-AA), secreted by the bacteria in inf…
View article: Cloning and Mutagenesis of AmpC<em> β</em>–lactamase in <em>Pseudomonas aeruginosa </em>for the Identification of Targetable Allosteric Sites
Cloning and Mutagenesis of AmpC<em> β</em>–lactamase in <em>Pseudomonas aeruginosa </em>for the Identification of Targetable Allosteric Sites Open
The opportunistic pathogen Pseudomonas aeruginosa is responsible for numerous chronic infections and has a case fatality rate of 50%. All P. aeruginosa strains express β–lactamase, an enzyme capable of inactivating β–…
View article: Toward a Comprehensive Analysis of Posttranscriptional Regulatory Networks: a New Tool for the Identification of Small RNA Regulators of Specific mRNAs
Toward a Comprehensive Analysis of Posttranscriptional Regulatory Networks: a New Tool for the Identification of Small RNA Regulators of Specific mRNAs Open
With the recognition of the importance of posttranscriptional regulation mediated by bacterial small RNAs (sRNAs), their contribution to global gene expression regulatory networks needs to be addressed in a truly comprehensive manner. Whil…
View article: Transcriptional Responses of Pseudomonas aeruginosa to Inhibition of Lipoprotein Transport by a Small Molecule Inhibitor
Transcriptional Responses of Pseudomonas aeruginosa to Inhibition of Lipoprotein Transport by a Small Molecule Inhibitor Open
A key set of lipoprotein transport components, LolCDE, were inhibited by both a small molecule as well as genetic downregulation of their expression. The data show a unique signature in the Pseudomonas aeruginosa transcriptome in response …
View article: Species-specific recruitment of transcription factors dictates toxin expression
Species-specific recruitment of transcription factors dictates toxin expression Open
Tight and coordinate regulation of virulence determinants is essential for bacterial biology and involves dynamic shaping of transcriptional regulatory networks during evolution. The horizontally transferred two-partner secretion system Ex…
View article: Correct Sorting of Lipoproteins into the Inner and Outer Membranes of Pseudomonas aeruginosa by the Escherichia coli LolCDE Transport System
Correct Sorting of Lipoproteins into the Inner and Outer Membranes of Pseudomonas aeruginosa by the Escherichia coli LolCDE Transport System Open
Gram-negative bacteria build their outer membranes (OM) from components that are initially located in the inner membrane (IM). A fraction of lipoproteins is transferred to the OM by the transport machinery consisting of LolABCDE proteins. …
View article: Insertion sequences drive the emergence of a highly adapted human pathogen
Insertion sequences drive the emergence of a highly adapted human pathogen Open
Pseudomonas aeruginosa is a highly adaptive opportunistic pathogen that can have serious health consequences in patients with lung disorders. Taxonomic outliers of P. aeruginosa of environmental origin have recently emerged as infectious f…
View article: Inhibition of <i>Pseudomonas aeruginosa</i> and <i>Mycobacterium tuberculosis</i> disulfide bond forming enzymes
Inhibition of <i>Pseudomonas aeruginosa</i> and <i>Mycobacterium tuberculosis</i> disulfide bond forming enzymes Open
Summary In bacteria, disulfide bonds confer stability on many proteins exported to the cell envelope or beyond, including bacterial virulence factors. Thus, proteins involved in disulfide bond formation represent good targets for the devel…
View article: Insertion sequences drive the emergence of a highly adapted human pathogen
Insertion sequences drive the emergence of a highly adapted human pathogen Open
Taxonomic outliers of Pseudomonas aeruginosa of environmental origin have recently emerged as infectious for humans. Here we present the first genome-wide analysis of an isolate that caused fatal hemorrhagic pneumonia. We demonstrate that,…
View article: cAMP and Vfr Control Exolysin Expression and Cytotoxicity of Pseudomonas aeruginosa Taxonomic Outliers
cAMP and Vfr Control Exolysin Expression and Cytotoxicity of Pseudomonas aeruginosa Taxonomic Outliers Open
The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are contr…
View article: Probing the sRNA regulatory landscape of <i>P. aeruginosa</i> : post‐transcriptional control of determinants of pathogenicity and antibiotic susceptibility
Probing the sRNA regulatory landscape of <i>P. aeruginosa</i> : post‐transcriptional control of determinants of pathogenicity and antibiotic susceptibility Open
Summary During environmental adaptation bacteria use small regulatory RNAs (sRNAs) to repress or activate expression of a large fraction of their proteome. We extended the use of the in vivo RNA proximity ligation method toward probing glo…
View article: Complexity of Complement Resistance Factors Expressed by <i>Acinetobacter baumannii</i> Needed for Survival in Human Serum
Complexity of Complement Resistance Factors Expressed by <i>Acinetobacter baumannii</i> Needed for Survival in Human Serum Open
Acinetobacter baumannii is a bacterial pathogen with increasing impact in healthcare settings, due in part to this organism’s resistance to many antimicrobial agents, with pneumonia and bacteremia as the most common manifestations of disea…
View article: Conjugative type IVb pilus recognizes lipopolysaccharide of recipient cells to initiate PAPI-1 pathogenicity island transfer in Pseudomonas aeruginosa
Conjugative type IVb pilus recognizes lipopolysaccharide of recipient cells to initiate PAPI-1 pathogenicity island transfer in Pseudomonas aeruginosa Open
These results suggest that a PAPI-1 exclusion mechanism exists in P. aeruginosa that might serve to regulate the avoidance of uncontrolled expansions of the bacterial genome.
View article: <i>Pseudomonas aeruginosa</i> Pore-Forming Exolysin and Type IV Pili Cooperate To Induce Host Cell Lysis
<i>Pseudomonas aeruginosa</i> Pore-Forming Exolysin and Type IV Pili Cooperate To Induce Host Cell Lysis Open
Clinical strains of Pseudomonas aeruginosa lacking the type III secretion system genes employ a toxin, exolysin (ExlA), for host cell membrane disruption. Here, we demonstrated that ExlA export requires a predicted outer membrane protein, …
View article: Transcriptional Responses of Escherichia coli to a Small-Molecule Inhibitor of LolCDE, an Essential Component of the Lipoprotein Transport Pathway
Transcriptional Responses of Escherichia coli to a Small-Molecule Inhibitor of LolCDE, an Essential Component of the Lipoprotein Transport Pathway Open
In Gram-negative bacteria, a dedicated machinery consisting of LolABCDE components targets lipoproteins to the outer membrane. We used a previously identified small-molecule inhibitor of the LolCDE complex of Escherichia coli to assess the…