Stephen Mann
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View article: Bioactive Artificial Cells as Autonomous Metabolic Actuators Enable Bidirectional Communication with Tumor Cells
Bioactive Artificial Cells as Autonomous Metabolic Actuators Enable Bidirectional Communication with Tumor Cells Open
Artificial cells (ACs) offer a powerful platform to reprogram metabolic signaling in complex tissue environments by replicating key biological functions without the full complexity of living cells. However, achieving autonomous metabolite …
View article: Self-assembly of hybrid 3D cultures by integrating living and synthetic cells
Self-assembly of hybrid 3D cultures by integrating living and synthetic cells Open
Self-assembly is a fundamental property of living matter that drives the three-dimensional organization of cell collectives such as tissues and organs. Here, the co-assembly of synthetic and natural cells is leveraged to create hybrid livi…
View article: Preference for Face‐to‐Face Contraceptive Service Delivery Post‐ <scp>COVID</scp> ‐19 Pandemic: A Cross‐Sectional Study
Preference for Face‐to‐Face Contraceptive Service Delivery Post‐ <span>COVID</span> ‐19 Pandemic: A Cross‐Sectional Study Open
Objective To measure the prevalence of, and social positions associated with, preference for solely face‐to‐face contraceptive service delivery among women and people assigned female at birth in post‐COVID‐19 pandemic England. Design Cross…
View article: Continuous Light‐Induced Water Oxidation in Polyoxometalate‐Based Photocatalytic Protocells and Prototissues
Continuous Light‐Induced Water Oxidation in Polyoxometalate‐Based Photocatalytic Protocells and Prototissues Open
The bottom‐up construction of leaf‐like materials capable of using visible light and water to produce oxygen and chemical energy is a major goal of artificial photosynthesis. In this work, we show that robust artificial cells ( protocells …
View article: Rapid and high-yield recovery of plasma-derived extracellular vesicles using modified chromatography with soluble protein depletion for biomarker discovery
Rapid and high-yield recovery of plasma-derived extracellular vesicles using modified chromatography with soluble protein depletion for biomarker discovery Open
Extracellular vesicles (EVs) are critical mediators of intercellular communication by transferring proteins, lipid and nucleic acids between cells. EVs in biofluids, particularly blood, have gathered significant interest as potential bioma…
View article: Cytomimetic calcification in chemically self-regulated prototissues
Cytomimetic calcification in chemically self-regulated prototissues Open
The fabrication of cytomimetic materials capable of orchestrated and adaptive functions remains a significant challenge in bottom-up synthetic biology. Inspired by the cell/matrix integration of living bone, here we covalently tether distr…
View article: Droplet microrobotic structures for compartmentalised biochemistry and organoid engineering
Droplet microrobotic structures for compartmentalised biochemistry and organoid engineering Open
Microrobotic structures formed from liquid materials offer certain advantages of reconfigurability and biochemical compatibility at microscopic scales. Here, we use electrothermally driven flow dynamics to prepare single or multi-unit reco…
View article: Adaptive ATP-induced molecular condensation in membranized protocells
Adaptive ATP-induced molecular condensation in membranized protocells Open
Liquid–liquid phase separation (LLPS) has been achieved in various cytomimetic (protocell) models, but controlling molecular condensation using noninert crowders to systematically alter protocell function remains challenging. Intracellular…
View article: Examining Students’ Perceptions of Equity in Informal STEM Education: A Community-Engaged, Visual Method
Examining Students’ Perceptions of Equity in Informal STEM Education: A Community-Engaged, Visual Method Open
This report from the field presents a community-engaged, visual method for evaluating students’ perceptions of equity in Maker Club, an informal STEM (Science, Technology, Math, and Engineering) education program in Ohio. The purpose of th…
View article: Advancing Technology for Humanity and Earth (+Water+Air)
Advancing Technology for Humanity and Earth (+Water+Air) Open
As technology advances, the integration of physical, virtual, and social worlds has led to a complex landscape of ``Realities'' such as Virtual Reality (VR), Augmented Reality (AR), metaverse, spatial computing, and other emerging paradigm…
View article: Dynamic Co‐Clustering and Self‐Sorting in Interactive Protocell Populations
Dynamic Co‐Clustering and Self‐Sorting in Interactive Protocell Populations Open
The design and implementation of collective actions in model protocell communities is an on‐going challenge in synthetic protobiology. Herein, we covalently graft alginate or chitosan onto the outer surface of semipermeable enzyme‐containi…
View article: Dynamic Co‐Clustering and Self‐Sorting in Interactive Protocell Populations
Dynamic Co‐Clustering and Self‐Sorting in Interactive Protocell Populations Open
The design and implementation of collective actions in model protocell communities is an on‐going challenge in synthetic protobiology. Herein, we covalently graft alginate or chitosan onto the outer surface of semipermeable enzyme‐containi…
View article: The P301L tau mutation alters extracellular vesicle biogenesis in astrocyte and contributes to astrocyte‐mediated tau pathology in a human iPSC‐derived model of tauopathies
The P301L tau mutation alters extracellular vesicle biogenesis in astrocyte and contributes to astrocyte‐mediated tau pathology in a human iPSC‐derived model of tauopathies Open
Background Tauopathies are a group of neurodegenerative disorders which are characterized by the accumulation of abnormal tau protein in the brain. However, the mechanistic understanding of pathogenic tau formation and spread within the br…
View article: Self-assembly of hybrid 3D cultures by integrating living and synthetic cells
Self-assembly of hybrid 3D cultures by integrating living and synthetic cells Open
Self-assembly, a fundamental property of living matter, drives the interconnected cellular organization of tissues. Synthetic cell models have been developed as bionic materials to mimic inherent cellular features such as self-assembly. He…
View article: Reprogramming macrophages with R848-loaded artificial protocells to modulate skin and skeletal wound healing
Reprogramming macrophages with R848-loaded artificial protocells to modulate skin and skeletal wound healing Open
After tissue injury, inflammatory cells are rapidly recruited to the wound where they clear microbes and other debris, and coordinate the behaviour of other cell lineages at the repair site in both positive and negative ways. In this study…
View article: Functional Integration of Synthetic Cells into 3D Microfluidic Devices for Artificial Organ‐On‐Chip Technologies
Functional Integration of Synthetic Cells into 3D Microfluidic Devices for Artificial Organ‐On‐Chip Technologies Open
Microfluidics plays a pivotal role in organ‐on‐chip technologies and in the study of synthetic cells, especially in the development and analysis of artificial cell models. However, approaches that use synthetic cells as integral functional…
View article: Protocell Flow Reactors for Enzyme and Whole‐Cell Mediated Biocatalysis
Protocell Flow Reactors for Enzyme and Whole‐Cell Mediated Biocatalysis Open
The design and construction of continuous flow biochemical reactors comprising immobilized biocatalysts have generated great interest in the efficient synthesis of value‐added chemicals. Living cells use compartmentalization and reaction‐d…
View article: Autonomous Development of Compositional Diversity in Self‐Spreading Flat Protocells
Autonomous Development of Compositional Diversity in Self‐Spreading Flat Protocells Open
An experimental pathway to the spontaneous generation of compositionally diverse synthetic protocells is presented. The pathway is initiated by flat giant unilamellar vesicles (FGUVs) that originate from compositionally different multilame…
View article: DNA as a universal chemical substrate for computing and data storage
DNA as a universal chemical substrate for computing and data storage Open
DNA computing and DNA data storage are emerging fields that are unlocking new possibilities in information technology and diagnostics. These approaches use DNA molecules as a computing substrate or a storage medium, offering nanoscale comp…
View article: Signal Transduction in Artificial Cells (Small Methods 12/2023)
Signal Transduction in Artificial Cells (Small Methods 12/2023) Open
Inside Back Cover In article number 2300231, Stephen Mann, Hongjing Dou, and co-workers discuss recent approaches for the integration of signal transduction modules in model protocells prepared by bottom-up construction. Transducers with i…
View article: Molecular tuning of DNA framework-programmed silicification by cationic silica cluster attachment
Molecular tuning of DNA framework-programmed silicification by cationic silica cluster attachment Open
The organizational complexity of biominerals has long fascinated scientists seeking to understand biological programming and implement new developments in biomimetic materials chemistry. Nonclassical crystallization pathways have been obse…
View article: Autonomous oscillatory movement of sensory protocells in stratified chemical media
Autonomous oscillatory movement of sensory protocells in stratified chemical media Open
The taxis of microscale objects in response to environmental chemical gradients represents a fundamental step toward the construction and programming of motile cytomimetic agents. Here, we demonstrate that various modes of autonomous oscil…