Stephen McIlfatrick
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View article: A micro-fabricated device (microICSI) improves porcine blastocyst development and procedural efficiency for both porcine intracytoplasmic sperm injection and human microinjection
A micro-fabricated device (microICSI) improves porcine blastocyst development and procedural efficiency for both porcine intracytoplasmic sperm injection and human microinjection Open
View article: Characterisation of transgenic pigs expressing a human T cell‐depleting anti‐CD2 monoclonal antibody
Characterisation of transgenic pigs expressing a human T cell‐depleting anti‐CD2 monoclonal antibody Open
Background Pig islet xenotransplantation is a potential treatment for type 1 diabetes. We have shown that maintenance immunosuppression is required to protect genetically modified (GM) porcine islet xenografts from T cell‐mediated rejectio…
View article: Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts
Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts Open
Mitochondrial DNA (mtDNA) deficiency correlates with poor oocyte quality and fertilisation failure. However, the supplementation of mtDNA deficient oocytes with extra copies of mtDNA improves fertilisation rates and embryo development. The…
View article: Does supplementation of oocytes with additional mtDNA influence developmental outcome?
Does supplementation of oocytes with additional mtDNA influence developmental outcome? Open
View article: Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts
Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts Open
View article: Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation
Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation Open
Mitochondrial DNA (mtDNA) methylation in vertebrates has been hotly debated for over 40 years. Most contrasting results have been reported following bisulfite sequencing (BS-seq) analyses. We addressed whether BS-seq experimental and analy…
View article: Additional file 2 of Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts
Additional file 2 of Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts Open
Additional file 2: Table S1. Summary statistics from Sus scrofa oocyte and blastocyst WGBS data analyses. Table S2. A list of DMRs between ICSI- and mICSI-derived blastocysts commonly identified by three DMR callers. Table S3. GO enrichmen…
View article: Targeted insertion of an anti-CD2 monoclonal antibody transgene into the GGTA1 locus in pigs using FokI-dCas9
Targeted insertion of an anti-CD2 monoclonal antibody transgene into the GGTA1 locus in pigs using FokI-dCas9 Open
Xenotransplantation from pigs has been advocated as a solution to the perennial shortage of donated human organs and tissues. CRISPR/Cas9 has facilitated the silencing of genes in donor pigs that contribute to xenograft rejection. However,…