Stephen Perusini
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View article: Supplementary Figures 1-4, Table 1 from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers
Supplementary Figures 1-4, Table 1 from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers Open
PDF file - 1.2MB
View article: Data from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers
Data from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers Open
Excessive signaling from the Wnt pathway is associated with numerous human cancers. Using a high throughput screen designed to detect inhibitors of Wnt/β-catenin signaling, we identified a series of acyl hydrazones that act downstream of t…
View article: Data from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers
Data from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers Open
Excessive signaling from the Wnt pathway is associated with numerous human cancers. Using a high throughput screen designed to detect inhibitors of Wnt/β-catenin signaling, we identified a series of acyl hydrazones that act downstream of t…
View article: Supplementary Figures 1-4, Table 1 from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers
Supplementary Figures 1-4, Table 1 from Wnt Inhibitor Screen Reveals Iron Dependence of β-Catenin Signaling in Cancers Open
PDF file - 1.2MB
View article: Environmental Surface Contamination With Monkeypox Virus in the Ambulatory Setting in Toronto, Canada
Environmental Surface Contamination With Monkeypox Virus in the Ambulatory Setting in Toronto, Canada Open
This article describes the results of environmental sampling in out-patient clinics where adults with human monkeypox were evaluated. Viral DNA was detected at low concentrations on high-touch surfaces. Hospital-grade cleaning wipes reduce…
View article: Monkeypox Virus Detection in Different Clinical Specimen Types
Monkeypox Virus Detection in Different Clinical Specimen Types Open
A global monkeypox outbreak began in May 2022. Limited data exist on specimen type performance in associated molecular diagnostics. Consequently, a diverse range of specimen sources were collected in the initial weeks of the outbreak in On…
View article: Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern
Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern Open
During the coronavirus disease 2019 (COVID-19) pandemic, several variants of concern (VOCs) have been detected, for example, B.1.1.7, B.1.351, P.1, and B.1.617.2. The VOCs pose a threat to public health efforts to control the spread of the…
View article: Impact of coronavirus disease 2019 (COVID-19) pre-test probability on positive predictive value of high cycle threshold severe acute respiratory coronavirus virus 2 (SARS-CoV-2) real-time reverse transcription polymerase chain reaction (RT-PCR) test results
Impact of coronavirus disease 2019 (COVID-19) pre-test probability on positive predictive value of high cycle threshold severe acute respiratory coronavirus virus 2 (SARS-CoV-2) real-time reverse transcription polymerase chain reaction (RT-PCR) test results Open
Objectives: Performance characteristics of SARS-CoV-2 nucleic acid detection assays are understudied within contexts of low pre-test probability, including screening asymptomatic persons without epidemiological links to confirmed cases, or…
View article: Real-time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with Variants of Concern
Real-time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with Variants of Concern Open
The N501Y amino acid mutation caused by a single point substitution A23063T in the spike gene of SARS-CoV2 is possessed by the three most common variants of concern - B.1.1.7, B.1.351, and P.1. A rapid screening tool using this mutation is…
View article: Impact of COVID-19 pre-test probability on positive predictive value of high cycle threshold SARS-CoV-2 real-time reverse transcription PCR test results
Impact of COVID-19 pre-test probability on positive predictive value of high cycle threshold SARS-CoV-2 real-time reverse transcription PCR test results Open
Background Performance characteristics of SARS-CoV-2 nucleic acid detection assays are understudied within contexts of low pre-test probability, including screening asymptomatic persons without epidemiological links to confirmed cases, or …
View article: Presence of Flavivirus Antibodies Does Not Lead to a Greater Number of Symptoms in a Small Cohort of Canadian Travelers Infected with Zika Virus
Presence of Flavivirus Antibodies Does Not Lead to a Greater Number of Symptoms in a Small Cohort of Canadian Travelers Infected with Zika Virus Open
Zika virus (ZIKV) is a mosquito-borne flavivirus associated with a febrile illness as well as severe complications, including microcephaly and Guillain-Barré Syndrome. Antibody cross-reactivity between flaviviruses has been documented, and…
View article: Hepatitis C Core-Antigen Testing from Dried Blood Spots
Hepatitis C Core-Antigen Testing from Dried Blood Spots Open
In order to expand hepatitis C virus (HCV) screening, a change in the diagnostic paradigm is warranted to improve accessibility and decrease costs, such as utilizing dried blood spot (DBS) collection. In our study, blood from 68 patients w…
View article: Use of Genome Sequencing to Define Institutional Influenza Outbreaks, Toronto, Ontario, Canada, 2014–15
Use of Genome Sequencing to Define Institutional Influenza Outbreaks, Toronto, Ontario, Canada, 2014–15 Open
Adequacy of the current clinical definition of institutional influenza outbreaks is unclear. We performed a retrospective genome sequencing and epidemiologic analysis of institutional influenza outbreaks that occurred during the 2014-15 in…
View article: Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing
Evaluation of Altona Diagnostics RealStar Zika Virus Reverse Transcription-PCR Test Kit for Zika Virus PCR Testing Open
With the emerging Zika virus (ZIKV) epidemic, accessible real-time reverse transcription-PCR (rRT-PCR) assays are needed to streamline testing. The commercial Altona Diagnostics RealStar ZIKV rRT-PCR test kit (Altona PCR) has been approved…
View article: Molecular Epidemiological Investigation of Human Parainfluenza 3 Virus Outbreak in a Pediatric Bone Marrow Transplantation Unit
Molecular Epidemiological Investigation of Human Parainfluenza 3 Virus Outbreak in a Pediatric Bone Marrow Transplantation Unit Open
Background Human parainfluenza virus 3 (hPIV3), a common cause of respiratory infections in children, can cause nosocomial outbreaks in patients undergoing hematopoietic stem cell transplantation, resulting in significant morbidity and mor…
View article: The Role of Whole Genome Sequencing in Defining Institutional Influenza Outbreaks
The Role of Whole Genome Sequencing in Defining Institutional Influenza Outbreaks Open
Influenza outbreaks in long-term care facilities are common and a major source of morbidity and mortality in older adults. The adequacy of the current clinical definition for identifying institutional influenza outbreaks is unclear. We per…
View article: Epidemiology of Enterovirus D68 in Ontario
Epidemiology of Enterovirus D68 in Ontario Open
In August 2014, children's hospitals in Kansas City, Missouri and Chicago, Illinois notified the Centers for Disease Control and Prevention (CDC) about increased numbers of pediatric patients hospitalized with severe respiratory illness (S…