Stephen A. Adam
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Expression of vimentin intermediate filaments in epithelial cells promotes cell migration and cell matrix interaction in 3D Open
During a variety of physiological and pathological processes, such as development, wound healing, and tumor progression, epithelial cells collectively invade into their surroundings. Vimentin intermediate filaments (VIFs) are often observe…
View article: Vimentin undergoes liquid–liquid phase separation to form droplets which wet and stabilize actin fibers
Vimentin undergoes liquid–liquid phase separation to form droplets which wet and stabilize actin fibers Open
The cytoskeleton is composed of F-actin, microtubules, and intermediate filaments (IFs). Vimentin is one of the most ubiquitous and well-studied IFs. It is involved in many activities including wound healing, tissue fibrosis, and cancer me…
View article: Roles of the lamin A-specific tail region in the localization to sites of nuclear envelope rupture
Roles of the lamin A-specific tail region in the localization to sites of nuclear envelope rupture Open
The nuclear lamina (NL) lines the nuclear envelope (NE) to maintain nuclear structure in metazoan cells. The major NL components, the nuclear lamins contribute to the protection against NE rupture induced by mechanical stress. Lamin A (LA)…
Phase separated liquid vimentin droplets stabilize actin fibers through wetting Open
The cytoskeleton is composed of F-actin, microtubules, and intermediate filaments (IFs). Vimentin is the most ubiquitous IF. It is involved in wound healing, tissue fibrosis and cancer metastasis, all of which require rapid vimentin filame…
Nuclear lamin A-associated proteins are required for centromere assembly Open
Summary Many Lamin A-associated proteins (LAAP’s) that are key constituents of the nuclear envelope (NE), assemble at the “core” domains of chromosomes during NE reformation and mitotic exit. However, the identity and function of the chrom…
View article: Delayed localization of A-type lamins to the rupture sites in Hutchinson–Gilford progeria syndrome
Delayed localization of A-type lamins to the rupture sites in Hutchinson–Gilford progeria syndrome Open
The nuclear lamina (NL) lines the nuclear envelope (NE) to maintain nuclear structure in metazoan cells. The major NL components, the nuclear lamins contribute to the protection against NE rupture induced by mechanical stress. Lamin A (LA)…
Comment on hess-2022-320 Open
Abstract. The majority of ungauged regions around the world are in protected areas and rivers with non-perennial flow regimes, which are vital to water security and conservation. There is a limited amount of ground data available in such r…
High quality mapping of chromatin at or near the nuclear lamina from small numbers of cells reveals cell cycle and developmental changes of chromatin at the nuclear periphery Open
The chromatin associated with the nuclear lamina (NL) is referred to as lamina-associated domains (LADs). Here, we present an adaptation of the tyramide-signal amplification sequencing (TSA-seq) protocol, which we call chromatin pull down-…
Expression of vimentin alters cell mechanics, cell-cell adhesion, and gene expression profiles suggesting the induction of a hybrid EMT in human mammary epithelial cells Open
Vimentin is a Type III intermediate filament (VIF) cytoskeletal protein that regulates the mechanical and migratory behavior of cells. Its expression is considered to be a marker for the epithelial to mesenchymal transition (EMT) that take…
Comment on hess-2022-320 Open
The majority of ungauged regions around the world are in protected areas and rivers with non-perennial flow regimes, which are vital to water security and conservation. There is a limited amount of ground data available in such regions, ma…
Supplementary material to "Streamflow Estimation in Ungauged Regions using Machine Learning: Quantifying Uncertainties in Geographic Extrapolation" Open
Figure S1.Locations of catchments for (a) raw GSIM dataset; (b) after the first step selection; and (c) the second step selection.
Streamflow Estimation in Ungauged Regions using Machine Learning: Quantifying Uncertainties in Geographic Extrapolation Open
The majority of ungauged regions around the world are in protected areas and rivers with non-perennial flow regimes, which are vital to water security and conservation. There is a limited amount of ground data available in such regions, ma…
Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS Open
In mammalian cell nuclei, the nuclear lamina (NL) underlies the nuclear envelope (NE) to maintain nuclear structure. The nuclear lamins, the major structural components of the NL, are involved in the protection against NE rupture induced b…
Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells Open
Cytoplasmic keratins couple with SUN2 and lamin A at the nuclear envelope and maintain plectin, LINC complex, lamin and lamin-associated protein levels in colonic epithelial cells. Loss of keratin 8 has no major impact on nuclear shape and…
Espin overexpression causes stereocilia defects and provides an anti‐capping effect on actin polymerization Open
Stereocilia are actin‐based projections of hair cells that are arranged in a step like array, in rows of increasing height, and that constitute the mechanosensory organelle used for the senses of hearing and balance. In order to function p…
View article: Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole-cell mechanics
Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole-cell mechanics Open
Significance Interactions between the cell nucleus and cytoskeleton regulate cell mechanics and are facilitated by the interplay between the nuclear lamina and linker of nucleoskeleton and cytoskeleton (LINC) complexes. To date, the specif…
Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex Open
Significance Filamentous actin (F-actin) and vimentin intermediate filaments (VIFs) are two major cytoskeletal components; they are generally thought to be spatially compartmentalized and to have distinctly different and independent functi…
Nuclear lamins: Structure and function in mechanobiology Open
Nuclear lamins are type V intermediate filament proteins that polymerize into complex filamentous meshworks at the nuclear periphery and in less structured forms throughout the nucleoplasm. Lamins interact with a wide range of nuclear prot…
Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS Open
In mammalian cell nuclei, the nuclear lamina (NL) underlies the nuclear envelope (NE) to maintain nuclear structure. The nuclear lamins, the major structural components of the NL, are involved in the protection against NE rupture induced b…
Vimentin Intermediate Filaments and Filamentous Actin Form Unexpected Interpenetrating Networks That Redefine the Cell Cortex Open
Vimentin intermediate filaments (VIFs) and F-actin are filamentous cytoskeletal proteins generally thought to form completely independent networks that have vastly different properties and functions. Here, we show that, unexpectedly, there…
Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole cell mechanics Open
The ability of a cell to regulate its mechanical properties is central to its function. Emerging evidence suggests that interactions between the cell nucleus and cytoskeleton influence cell mechanics through poorly understood mechanisms. H…
Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins Open
Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using datasets prepared from subpixel and segmentation analyses of 3D–structured illumination microscopy images of WT and lamin isoform knockout m…
High quality mapping of chromatin at or near the nuclear lamina for small numbers of cells Open
The chromatin associated with the nuclear lamina (NL) is referred to as Lamina-Associated Domains (LADs). While mapping of this feature has been done using various technologies, technical limitations exist for each of the methods. Here, we…
An APEX2 proximity ligation method for mapping interactions with the nuclear lamina Open
The nuclear lamina (NL) is a meshwork found beneath the inner nuclear membrane. The study of the NL is hindered by the insolubility of the meshwork and has driven the development of proximity ligation methods to identify the NL-associated/…
Keratins couple with the nuclear lamina and regulate proliferation in colonic epithelial cells Open
Keratin intermediate filaments (IFs) convey mechanical stability and protection against stress to epithelial cells, and may participate in nuclear structure and organization. Keratins are important for colon health as observed in keratin 8…
Computational analysis of lamin isoform interactions with nuclear pore complexes Open
Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using data sets prepared from sub-pixel and segmentation analyses of 3D-Structured Illumination Microscopy images of WT and lamin isoform knockout…
The versatility of Ascorbate Peroxidase-aided mapping uncovers insights of the nuclear lamina interactions and function Open
The nuclear lamina (NL) is a proteinaceous network found beneath the inner nuclear membrane. The NL is linked to a number of dynamic cellular activities including chromatin organization, transcription and RNA/protein trafficking through nu…
View article: Vimentin protects cells against nuclear rupture and DNA damage during migration
Vimentin protects cells against nuclear rupture and DNA damage during migration Open
Mammalian cells frequently migrate through tight spaces during normal embryogenesis, wound healing, diapedesis, or in pathological situations such as metastasis. Nuclear size and shape are important factors in regulating the mechanical pro…
Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation Open
The nucleus houses, organizes, and protects chromatin to ensure genome integrity and proper gene expression, but how the nucleus adapts mechanically to changes in the extracellular environment is poorly understood. Recent studies have reve…
Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation Open
The nucleus houses, organizes, and protects chromatin to ensure genome integrity and proper gene expression, but how the nucleus adapts mechanically to changes in the extracellular environment is poorly understood. Recent studies have reve…