Explanipedia

Transcriptomic time course of skeletal muscle disuse and rehabilitation in middle‐aged adults Open

Zachary D. Von Ruff, Sean P. Kilroe, Erik D. Marchant, Emily Arentson‐Lantz, Steven G. Widen , et al. · 2025

Disuse drives rapid muscle atrophy and metabolic dysfunction. This study aimed to characterize phenotypic and transcriptomic skeletal muscle changes in middle‐aged individuals during disuse and rehabilitation. Eleven healthy middle‐aged ad…

Skeletal Muscle Transcriptome Alterations Related to Declining Physical Function in Older Mice Open

Ted G. Graber, Rosario Maroto, Jill K. Thompson, Steven G. Widen, Zhaohui Man , et al. · 2025

One inevitable consequence of aging is the gradual deterioration of physical function and exercise capacity, driven in part by the adverse effect of age on muscle tissue. We hypothesized that relationships exist between age-related differe…

Proactive One-Health surveillance reveals novel orthobunyaviruses and their potential vectors and reservoirs Open

Jean‐Paul Carrera, Carlos Lezcano-Coba, Josefrancisco Galué, William Marciel de Souza, Weng M. Ng , et al. · 2025

Mosquito-borne viruses pose a significant, global threat to human and animal health. Traditionally, arthropod-borne virus (arbovirus) surveillance has been retroactive following an outbreak, which limits the ability to predict and prevent …

Dehydration-induced Ae-Aper50 regulates midgut infection in Aedes aegypti mosquitoes Open

Anastasia Accoti, Margaret Becker, Angel Elma I. Abu, Julia Vulcan, Rao Jun , et al. · 2025

Climate change is predicted to increase the spread of mosquito-borne viruses, but genetic mechanisms underlying the influence of environmental variation on the ability of insect vectors to transmit human pathogens is unknown. In response t…

Cache Valley virus isolation from a horse in Veracruz State, Mexico Open

Gamaliel Ortega-Soriano, Jessica A. Plante, Veronica A. Fabela-Becerril, Ana Luz Gonzalez-Perez, Mario Solís-Hernández , et al. · 2024

Cache Valley virus (CVV) was isolated from a clinically presenting horse in the Mexican Gulf region. CVV is linked to neuroinvasive disease in humans and fetal demise and defects in sheep. This is the first association between CVV and dise…

Parsing the roles of DExD-box proteins DDX39A and DDX39B in alternative RNA splicing Open

Shefali Banerjee, Chloe K. Nagasawa, Steven G. Widen, Mariano A. García-Blanco · 2024

DExD-box RNA proteins DDX39A and DDX39B are highly homologous paralogs that are conserved in vertebrates. They are required for energy-driven reactions involved in RNA processing. Although we have some understanding of how their functions …

Multiple layers of innate immune response antagonism of SARS-CoV-2 Open

Fuchun Zhou, Sivakumar Periasamy, Nathaniel Jackson, Wan Sze Cheng, Ruben Soto Acosta , et al. · 2024

Several SARS-CoV-2 proteins have been shown to counteract the host innate immune response, mostly using in vitro protein expression, which may not fully reflect their role in the context of viral infection. In addition, while each viral pr…

Complete genome sequence of Koutango virus strain DakAnD5443 isolated from Tatera kempi in 1968 Open

Gabriel J. Haila, Jessica A. Plante, Steven G. Widen, David W. C. Beasley · 2023

Koutango virus (KOUV), a close relative of West Nile virus, is highly neuroinvasive in animal models and has been associated with human disease. The complete genome of the KOUV prototype strain DakAnD5443 is reported here and may facilitat…

Dehydration inducedAePer50regulates midgut infection inAe. aegypti Open

Anastasia Accoti, Margaret Becker, Angel Elma I. Abu, Julia Vulcan, Ruimei Yun , et al. · 2023

In the face of climate change, mosquitoes will experience evolving climates including longer periods of drought. An important physiological response to dry environments is the protection against water loss or dehydration, here defined as d…

Skeletal Muscle Transcriptome Alterations Related to Declining Physical Function in Older Mice Open

Ted G. Graber, Rosario Maroto, Jill K. Thompson, Steven G. Widen, Zhaohui Man , et al. · 2023

One inevitable consequence of aging is the gradual deterioration of physical function and exercise capacity, driven in part by the adverse effect of age on muscle tissue. We hypothesized that relationships exist between age-related differe…

The RNA helicase DDX39B activates FOXP3 RNA splicing to control T regulatory cell fate Open

Minato Hirano, Gaddiel Galarza-Muñoz, Chloe K. Nagasawa, Geraldine Schott, Liuyang Wang , et al. · 2023

Genes associated with increased susceptibility to multiple sclerosis (MS) have been identified, but their functions are incompletely understood. One of these genes codes for the RNA helicase DExD/H-Box Polypeptide 39B (DDX39B), which shows…

Author response: The RNA helicase DDX39B activates FOXP3 RNA splicing to control T regulatory cell fate Open

Minato Hirano, Gaddiel Galarza-Muñoz, Chloe K. Nagasawa, Geraldine Schott, Liuyang Wang , et al. · 2023

Differences in Genetic Diversity of Mammalian Tick-Borne Flaviviruses Open

Kassandra L. Carpio, Jill K. Thompson, Steven G. Widen, Jennifer K. Smith, Terry L. Juelich , et al. · 2023

The genetic diversities of mammalian tick-borne flaviviruses are poorly understood. We used next-generation sequencing (NGS) to deep sequence different viruses and strains belonging to this group of flaviviruses, including Central European…

Morphologic and Genetic Characterization of Ilheus Virus, a Potential Emergent Flavivirus in the Americas Open

Jessica A. Plante, Kenneth S. Plante, Vsevolod L. Popov, Divya P. Shinde, Steven G. Widen , et al. · 2023

Ilheus virus (ILHV) is a mosquito-borne flavivirus circulating throughout Central and South America and the Caribbean. It has been detected in several mosquito genera including Aedes and Culex, and birds are thought to be its primary ampli…

Cross-neutralization and viral fitness of SARS-CoV-2 Omicron sublineages Open

Hongjie Xia, Jason Yeung, Birte Kalveram, Cody J. Bills, John Yun-Chung Chen , et al. · 2023

The rapid evolution of SARS-CoV-2 Omicron sublineages mandates a better understanding of viral replication and cross-neutralization among these sublineages. Here we used K18-hACE2 mice and primary human airway cultures to examine the viral…

Skeletal muscle transcriptome is affected by age in severely burned mice Open

Juquan Song, Steven G. Widen, Steven E. Wolf, Amina EI Ayadi · 2022

Cross-neutralization and viral fitness of SARS-CoV-2 Omicron sublineages Open

Hongjie Xia, Jason Yeung, Birte Kalveram, Cody J. Bills, John Yun-Chung Chen , et al. · 2022

The rapid evolution of SARS-CoV-2 Omicron sublineages mandates a better understanding of viral replication and cross-neutralization among these sublineages. Here we used K18-hACE2 mice and primary human airway cultures to examine the viral…

Dual RNA-seq reveals a type 6 secretion system-dependent blockage of TNF-α signaling and BicA as a Burkholderia pseudomallei virulence factor important during gastrointestinal infection Open

Javier I. Sanchez-Villamil, Daniel Tapia, Nittaya Khakhum, Steven G. Widen, Alfredo G. Torres · 2022

Melioidosis is a disease caused by the Gram-negative bacillus Burkholderia pseudomallei (Bpm), commonly found in soil and water of endemic areas. Naturally acquired human melioidosis infections can result from either exposure…

Mechanistic insights gained from cell and molecular analysis of the neuroprotective potential of bioactive natural compounds in an immortalized hippocampal cell line Open

Harris A. Weisz, Deborah R. Boone, William S. Coggins, Gabrielle A. Edwards, Hannah E. Willey , et al. · 2022

Evaluating novel compounds for neuroprotective effects in animal models of traumatic brain injury (TBI) is a protracted, labor-intensive and costly effort. However, the present lack of effective treatment options for TBI, despite decades o…

Characterizing molecular subtypes of high-risk non-muscle-invasive bladder cancer in African American patients Open

Sungyong You, Minhyung Kim, Steven G. Widen, Alexander Yu, Gloria C. Galván , et al. · 2022

Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant Open

Yang Liu, Jianying Liu, Bryan A. Johnson, Hongjie Xia, Zhiqiang Ku , et al. · 2022

We report that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta spike mutation P681R plays a key role in the Alpha-to-Delta variant replacement during the coronavirus disease 2019 (COVID-19) pandemic. Delta SARS-CoV-2 eff…

A single intranasal dose of human parainfluenza virus type 3-vectored vaccine induces effective antibody and memory T cell response in the lungs and protects hamsters against SARS-CoV-2 Open

Philipp A. Ilinykh, Sivakumar Periasamy, Kai Huang, Natalia A. Kuzmina, Palaniappan Ramanathan , et al. · 2022

Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate Open

W. Samuel Fagg, Naiyou Liu, Ulrich Braunschweig, Karen Larissa Pereira de Castro, Xiaoting Chen , et al. · 2022

Alternative splicing is critical for development; however, its role in the specification of the three embryonic germ layers is poorly understood. By performing RNA-Seq on human embryonic stem cells (hESCs) and derived definitive endoderm, …

Dual RNA-seq reveals a type 6 secretion system-dependent blockage of TNF-α signaling and BicA as a Burkholderia pseudomallei virulence factor important during gastrointestinal infection Open

Javier I. Sanchez-Villamil, Daniel Tapia, Nittaya Khakum, Steven G. Widen, Alfredo G. Torres · 2022

Melioidosis is a disease caused by the Gram-negative bacillus Burkholderia pseudomallei ( Bpm ), commonly found in soil and water of endemic areas. Naturally acquired human melioidosis infections can result from either exposure through per…

Genetic Diversity Does Not Contribute to Attenuation of HeLa Passaged Wild-Type Yellow Fever Virus Strain French Viscerotropic Virus Open

Ashley E. Strother, Jill K. Thompson, Steven G. Widen, Alan D.T. Barrett · 2022

The disease yellow fever was prevented by two live attenuated vaccines, strains 17D and French neurotropic vaccine (FNV), derived by serial passage of wild-type (WT) strains Asibi and French Viscerotropic virus (FVV), respectively. Both 17…

The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity Open

Adam Hage, Preeti Bharaj, Sarah van Tol, María I. Giraldo, María González-Orozco , et al. · 2022

The RNA helicase DDX39B activates FOXP3 RNA splicing to control T regulatory cell fate Open

Minato Hirano, Gaddiel Galarza-Muñoz, Geraldine Schott, Liuyang Wang, Chloe K. Nagasawa , et al. · 2022

Summary Genes associated with increased susceptibility to multiple sclerosis (MS) have been identified, but their functions are incompletely understood. One of these genes codes for the RNA helicase DExD/H-Box Polypeptide 39B (DDX39B), whi…

RBFOX2 is required for establishing RNA regulatory networks essential for heart development Open

Sunil K. Verma, Vaibhav Deshmukh, Kaitlyn Thatcher, KarryAnne Belanger, Alexander M. Rhyner , et al. · 2022

Human genetic studies identified a strong association between loss of function mutations in RBFOX2 and hypoplastic left heart syndrome (HLHS). There are currently no Rbfox2 mouse models that recapitulate HLHS. Therefore, it is still unknow…

The N501Y spike substitution enhances SARS-CoV-2 infection and transmission Open

Yang Liu, Jianying Liu, Kenneth S. Plante, Jessica A. Plante, Xuping Xie , et al. · 2021

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants Open

Elizabeth Jaworski, Rose M. Langsjoen, Brooke Mitchell, Barbara M. Judy, Patrick C. Newman , et al. · 2021

High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and to identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving virus a…

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