Steven J. Ludtke
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View article: Engineered exosomes with KrasG12D specific siRNA in pancreatic cancer: a phase I study with immunological correlates
Engineered exosomes with KrasG12D specific siRNA in pancreatic cancer: a phase I study with immunological correlates Open
View article: Primary cortical neurons precipitate and extrude large mitochondria-associated calcium-phosphate sheets with a bone-precursor-like ultrastructure
Primary cortical neurons precipitate and extrude large mitochondria-associated calcium-phosphate sheets with a bone-precursor-like ultrastructure Open
Calcium-phosphate (CaP) is a ubiquitous inorganic compound that plays an important structural role in healthy bone and teeth formation, but its pathologic buildup can occur in dyshomeostatic calcium disorders like Alzheimer’s disease and L…
View article: The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge
The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge Open
Despite the rise of single particle cryo-electron microscopy (cryo-EM) as a premier method for resolving macromolecular structures at atomic resolution, methods to address molecular heterogeneity in vitrified samples have yet to reach matu…
View article: TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET
TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET Open
View article: KRAS<sup>G12D</sup>-Specific Targeting with Engineered Exosomes Reprograms the Immune Microenvironment to Enable Efficacy of Immune Checkpoint Therapy in PDAC Patients
KRAS<sup>G12D</sup>-Specific Targeting with Engineered Exosomes Reprograms the Immune Microenvironment to Enable Efficacy of Immune Checkpoint Therapy in PDAC Patients Open
Oncogenic KRAS drives initiation and maintenance of pancreatic ductal adenocarcinoma (PDAC). Here, we show that engineered exosomes with Kras G12D specific siRNA (iExoKras G12D ) reveal impressive biodistribution in pancreas with negligibl…
View article: TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET
TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET Open
Electron cryo-tomography (cryo-ET) is a powerful imaging tool that allows three-dimensional visualization of subcellular architecture. During morphological analysis, reliable tomogram segmentation can only be achieved through high-quality …
View article: Correction: Jiménez-Ortigosa et al. Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins. J. Fungi 2021, 7, 120
Correction: Jiménez-Ortigosa et al. Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins. J. Fungi 2021, 7, 120 Open
The authors wish to update the article title to “Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins” [...]
View article: Community recommendations on cryoEM data archiving and validation
Community recommendations on cryoEM data archiving and validation Open
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data pr…
View article: HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association
HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association Open
We report our findings on the assembly of the HIV-1 protein Vpu into soluble oligomers. Vpu is a key HIV-1 protein. It has been considered exclusively a single-pass membrane protein. Previous observations show that this protein forms stabl…
View article: Author Correction: Membrane translocation process revealed by in situ structures of type II secretion system secretins
Author Correction: Membrane translocation process revealed by in situ structures of type II secretion system secretins Open
View article: Correlative Cryo-FIB and Cryo-ET of Dendritic Spines and Synaptic Connections
Correlative Cryo-FIB and Cryo-ET of Dendritic Spines and Synaptic Connections Open
Direct electron detectors combined with Cryo-FIB milling have made sub-nanometer resolutions possible from cellular data with CryoET and subtomogram averaging.The major impediment to this technique is target localization within the cell.Th…
View article: Membrane translocation process revealed by in situ structures of type II secretion system secretins
Membrane translocation process revealed by in situ structures of type II secretion system secretins Open
The GspD secretin is the outer membrane channel of the bacterial type II secretion system (T2SS) which secrets diverse toxins that cause severe diseases such as diarrhea and cholera. GspD needs to translocate from the inner to the outer me…
View article: HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association
HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association Open
We report our findings on the assembly of the HIV-1 protein Vpu into soluble oligomers. Vpu is a key to HIV-1 protein. It has been considered exclusively a single-pass membrane protein. However, we revealed that this protein forms stable o…
View article: <i>In situ</i>structures of secretins from bacterial type II secretion system reveal their membrane interactions and translocation process
<i>In situ</i>structures of secretins from bacterial type II secretion system reveal their membrane interactions and translocation process Open
The GspD secretin is the outer membrane channel of the bacterial type II secretion system (T2SS) which secrets diverse effector proteins or toxins that cause severe diseases such as diarrhea and cholera. GspD needs to translocate from the …
View article: Conformational motions and ligand-binding underlying gating and regulation in IP3R channel
Conformational motions and ligand-binding underlying gating and regulation in IP3R channel Open
View article: Spatial definition of the human progesterone receptor-B transcriptional complex
Spatial definition of the human progesterone receptor-B transcriptional complex Open
View article: Precision requirements and data compression in CryoEM/CryoET
Precision requirements and data compression in CryoEM/CryoET Open
View article: Structural dynamics underlying gating and regulation in IP<sub>3</sub>R channel
Structural dynamics underlying gating and regulation in IP<sub>3</sub>R channel Open
Inositol-1,4,5-trisphosphate receptors (IP 3 Rs) are activated by IP 3 and Ca 2+ and their gating is regulated by various intracellular messengers that finely tune the channel activity. Here, using single particle cryo-EM analysis we deter…
View article: Cryo-ET of <i>Toxoplasma</i> parasites gives subnanometer insight into tubulin-based structures
Cryo-ET of <i>Toxoplasma</i> parasites gives subnanometer insight into tubulin-based structures Open
Significance Tubulin polymers are essential for a variety of cellular functions. Using cryo-ET, we reveal the 3D organization of the apical complex in Toxoplasma gondii , an intracellular eukaryote with tubulin-based structures, including …
View article: Using Cryo-ET to distinguish platelets during pre-acute myeloid leukemia from steady state hematopoiesis
Using Cryo-ET to distinguish platelets during pre-acute myeloid leukemia from steady state hematopoiesis Open
View article: Comprehensive structure and functional adaptations of the yeast nuclear pore complex
Comprehensive structure and functional adaptations of the yeast nuclear pore complex Open
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible …
View article: CX-MS Datasets for "Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex"
CX-MS Datasets for "Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex" Open
This repository contains chemical cross-linking mass spectrometry data of affinity-purified Yeast nuclear pore complexes. Data Files Description: NPC_XL_Identification_Inter_Crosslinked.csv: Inter-protein cross-links identified by pLink 2.…
View article: CX-MS Datasets for "Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex"
CX-MS Datasets for "Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex" Open
This repository contains chemical cross-linking mass spectrometry data of affinity-purified Yeast nuclear pore complexes. Data Files Description: NPC_XL_Identification_Inter_Crosslinked.csv: Inter-protein cross-links identified by pLink 2.…
View article: Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex
Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex Open
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible …
View article: Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex
Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex Open
Nuclear Pore Complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the yeast NPC in which the inner ring is resolved by cryo-EM at - helical resolution to show how flexible connectors ti…
View article: Cryo-ET distinguishes platelets during pre-acute myeloid leukemia from steady state hematopoiesis
Cryo-ET distinguishes platelets during pre-acute myeloid leukemia from steady state hematopoiesis Open
Early diagnosis of acute myeloid leukemia (AML) in the pre-leukemic stage remains a clinical challenge, as pre-leukemic patients show no symptoms, lacking any known morphological or numerical abnormalities in blood cells. Here, we demonstr…
View article: In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution
In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution Open
View article: Explore the complexity of proteins with an expanded CryoET data processing pipeline
Explore the complexity of proteins with an expanded CryoET data processing pipeline Open
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View article: Cryo-ET reveals two major tubulin-based cytoskeleton structures in<i>Toxoplasma gondii</i>
Cryo-ET reveals two major tubulin-based cytoskeleton structures in<i>Toxoplasma gondii</i> Open
SUMMARY In the obligate intracellular parasite, Toxoplasma gondii , the subpellicular microtubules (SPMTs) help maintain shape, while the apical conoid (also tubulin-based) is implicated in invasion. Here, we use cryo-electron tomography t…
View article: Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins
Cryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins Open
Fungal plasma membrane proteins have long been recognized as targets for the development of antifungal agents. Despite recent progress in experimental approaches and computational structural predictions, our knowledge of the structural dyn…