Steven L. Coon
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View article: Anatomical and Molecular Characterization of the Zebrafish Meninges
Anatomical and Molecular Characterization of the Zebrafish Meninges Open
The meninges are a set of connective tissue layers that surround the central nervous system, protecting the brain from mechanical shock, supporting its buoyancy, guarding it from infection and injury, and maintaining brain homeostasis. Des…
View article: Author Correction: TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A
Author Correction: TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A Open
View article: High-frequency variants in PKA signaling-related genes within a large pediatric cohort with obesity or metabolic abnormalities
High-frequency variants in PKA signaling-related genes within a large pediatric cohort with obesity or metabolic abnormalities Open
Introduction Pediatric obesity has steadily increased in recent decades. Large-scale genome-wide association studies (GWAS) conducted primarily in Eurocentric adult populations have identified approximately 100 loci that predispose to obes…
View article: A reference human induced pluripotent stem cell line for large-scale collaborative studies
A reference human induced pluripotent stem cell line for large-scale collaborative studies Open
View article: Mitigating Diabetic Foot Ulcers: The Effect of Diet and Microbiome
Mitigating Diabetic Foot Ulcers: The Effect of Diet and Microbiome Open
To truly eliminate the possibility of diabetic foot ulcers, the elimination of the symptoms of diabetes is essential. There are many forms of diabetes and there is no one diet that is effective for all patients. It is essential that a prop…
View article: The astroglial and stem cell functions of adult rat folliculostellate cells
The astroglial and stem cell functions of adult rat folliculostellate cells Open
The mammalian pituitary gland is a complex organ consisting of hormone‐producing cells, anterior lobe folliculostellate cells (FSCs), posterior lobe pituicytes, vascular pericytes and endothelial cells, and Sox2 ‐expressing stem cells. We …
View article: Structural basis of 3′-end poly(A) RNA recognition by LARP1
Structural basis of 3′-end poly(A) RNA recognition by LARP1 Open
La-related proteins (LARPs) comprise a family of RNA-binding proteins involved in a wide range of posttranscriptional regulatory activities. LARPs share a unique tandem of two RNA-binding domains, La motif (LaM) and RNA recognition motif (…
View article: Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents
Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents Open
View article: TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A
TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A Open
View article: A reference induced pluripotent stem cell line for large-scale collaborative studies
A reference induced pluripotent stem cell line for large-scale collaborative studies Open
Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across res…
View article: Transcriptomic heterogeneity of <i>Sox2</i>-expressing pituitary cells
Transcriptomic heterogeneity of <i>Sox2</i>-expressing pituitary cells Open
The mammalian pituitary gland is a complex organ consisting of hormone-producing cells (HPC), nonhormonal folliculostellate cells (FSC) and pituicytes, vascular pericytes and endothelial cells, and putative Sox2 -expressing stem cells. Her…
View article: Developing biomarkers of TDP‐43‐related splicing dysfunction in frontotemporal dementia
Developing biomarkers of TDP‐43‐related splicing dysfunction in frontotemporal dementia Open
Background Frontotemporal dementia (FTD) is a rapidly progressive neurodegenerative disorder characterized by prominent atrophy of the frontal and temporal lobes and marked language and/or behavioral disturbances. Mislocalization of the TA…
View article: A fully automated FAIMS-DIA proteomic pipeline for high-throughput characterization of iPSC-derived neurons
A fully automated FAIMS-DIA proteomic pipeline for high-throughput characterization of iPSC-derived neurons Open
Fully automated proteomic pipelines have the potential to achieve deep coverage of cellular proteomes with high throughput and scalability. However, it is important to evaluate performance, including both reproducibility and ability to pro…
View article: Morphologic and Molecular Characterization of Adrenals and Adrenal Rest Affected by Congenital Adrenal Hyperplasia
Morphologic and Molecular Characterization of Adrenals and Adrenal Rest Affected by Congenital Adrenal Hyperplasia Open
Introduction Adrenocortical hyperplasia and adrenal rest tumor (ART) formation are common in congenital adrenal hyperplasia (CAH). Although driven by excessive corticotropin, much is unknown regarding the morphology and transformation of t…
View article: Detection of precisely edited CRISPR/Cas9 alleles through co-introduced restriction-fragment length polymorphisms
Detection of precisely edited CRISPR/Cas9 alleles through co-introduced restriction-fragment length polymorphisms Open
CRISPR/Cas9 is a powerful tool for producing genomic in sertions and del etions (indels) to interrogate gene function. Modified CRISPR/Cas9 protocols can produce targeted genetic changes that are more precise than indels, but founder recov…
View article: The PRKAR1B p.R115K Variant is Associated with Lipoprotein Profile in African American Youth with Metabolic Challenges
The PRKAR1B p.R115K Variant is Associated with Lipoprotein Profile in African American Youth with Metabolic Challenges Open
Context High childhood obesity rates coincide with increased incidence of nonalcoholic fatty liver disease (NAFLD) and other comorbidities. Understanding the genetics of susceptibility to obesity and its comorbidities could guide intervent…
View article: Single‐Cell RNA Sequencing Analysis of the <i>Drosophila</i> Larval Ventral Cord
Single‐Cell RNA Sequencing Analysis of the <i>Drosophila</i> Larval Ventral Cord Open
Drosophila provides a powerful genetic system and an excellent model to study the development and function of the nervous system. The fly's small brain and complex behavior has been instrumental in mapping neuronal circuits and elucidating…
View article: Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis
Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis Open
GRTH/DDX25 is a member of the DEAD-box family of RNA helicases that play an essential role in spermatogenesis. GRTH knock-in (KI) mice with the human mutant GRTH gene (R242H) show loss of the phospho-species from cytoplasm with preservatio…
View article: Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails
Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails Open
La-related protein 4 (LARP4) directly binds both poly(A) and poly(A)-binding protein (PABP). LARP4 was shown to promote poly(A) tail (PAT) lengthening and stabilization of individual mRNAs presumably by protection from deadenylation (Matti…
View article: Author response: Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails
Author response: Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails Open
View article: Resource: A multi‐species multi‐timepoint transcriptome database and webpage for the pineal gland and retina
Resource: A multi‐species multi‐timepoint transcriptome database and webpage for the pineal gland and retina Open
The website and database https://snengs.nichd.nih.gov provides RNA sequencing data from multi‐species analysis of the pineal glands from zebrafish ( Danio rerio ), chicken (White Leghorn), rat ( Rattus nove gicus ), mouse ( Mus musculus ),…
View article: OR22-07 Novel Variants in Protein Kinase a Signaling-Related Genes Identified in Obese Children with and Without NAFLD
OR22-07 Novel Variants in Protein Kinase a Signaling-Related Genes Identified in Obese Children with and Without NAFLD Open
Context: Nonalcoholic fatty liver disease (NAFLD) is estimated to affect nearly 10% of Americans age 2-19 and about 38% of those affected are obesei. NAFLD is characterized by triglyceride accumulation in hepatocytes and can progress to no…
View article: Circadian regulation and molecular role of the <i>Bsx</i> homeobox gene in the adult pineal gland
Circadian regulation and molecular role of the <i>Bsx</i> homeobox gene in the adult pineal gland Open
The pineal gland is a neuroendocrine organ responsible for production of the nocturnal hormone melatonin. A specific set of homeobox gene‐encoded transcription factors govern pineal development, and some are expressed in adulthood. The bra…
View article: The <i>Lhx4</i> homeobox transcript in the rat pineal gland: Adrenergic regulation and impact on transcripts encoding melatonin‐synthesizing enzymes
The <i>Lhx4</i> homeobox transcript in the rat pineal gland: Adrenergic regulation and impact on transcripts encoding melatonin‐synthesizing enzymes Open
Homeobox genes generally encode transcription factors involved in regulating developmental processes. In the pineal gland, a brain structure devoted to nocturnal melatonin synthesis, a number of homeobox genes are also expressed postnatall…
View article: Single Cell Sequencing of the Pineal Gland: The Next Chapter
Single Cell Sequencing of the Pineal Gland: The Next Chapter Open
The analysis of pineal cell biology has undergone remarkable development as techniques have become available which allow for sequencing of entire transcriptomes and, most recently, the sequencing of the transcriptome of individual cells. I…
View article: Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells
Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells Open
Understanding the physiology and pathology of an organ composed of a variety of cell populations depends critically on genome-wide information on each cell type. Here, we report single-cell transcriptome profiling of over 6,800 freshly dis…
View article: Corrigendum to: A new <i>cis</i>-acting regulatory element driving gene expression in the zebrafish pineal gland
Corrigendum to: A new <i>cis</i>-acting regulatory element driving gene expression in the zebrafish pineal gland Open
View article: Single-cell RNA sequencing of the mammalian pineal gland identifies two pinealocyte subtypes and cell type-specific daily patterns of gene expression
Single-cell RNA sequencing of the mammalian pineal gland identifies two pinealocyte subtypes and cell type-specific daily patterns of gene expression Open
The vertebrate pineal gland is dedicated to the production of the hormone melatonin, which increases at night to influence circadian and seasonal rhythms. This increase is associated with dramatic changes in the pineal transcriptome. Here,…
View article: A Comparative Genomic and Transcriptomic Survey Provides Novel Insights into N-Acetylserotonin Methyltransferase (ASMT) in Fish
A Comparative Genomic and Transcriptomic Survey Provides Novel Insights into N-Acetylserotonin Methyltransferase (ASMT) in Fish Open
Melatonin is a multifunctional bioactive molecule that plays comprehensive physiological roles in all living organisms. N-acetylserotonin methyltransferase (ASMT, also known as hydroxyindole O-methyltransferase or HIOMT) is the final enzym…
View article: SIRT1 is a transcriptional enhancer of the glucocorticoid receptor acting independently to its deacetylase activity
SIRT1 is a transcriptional enhancer of the glucocorticoid receptor acting independently to its deacetylase activity Open