Steven M. Pascal
YOU?
Author Swipe
View article: The Importance of Solution Studies for the Structural Characterization of the Enterovirus 5’ Cloverleaf
The Importance of Solution Studies for the Structural Characterization of the Enterovirus 5’ Cloverleaf Open
Enteroviruses initiate genomic replication via a highly conserved mechanism that is controlled by an RNA platform, also known as the 5’ cloverleaf (5’CL). Here, we present a biophysical analysis of the 5’CL conformation of three enteroviru…
View article: Correction: Pandey et al. Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis. Biomolecules 2023, 13, 667
Correction: Pandey et al. Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis. Biomolecules 2023, 13, 667 Open
Andrea M [...]
View article: An <i>E. coli</i> two-hybrid system to investigate human protein-protein interactions
An <i>E. coli</i> two-hybrid system to investigate human protein-protein interactions Open
The LexA-E. coli two-hybrid (LexA-E2H) system was initially developed to study interactions between microbial proteins in an Escherichia coli (E. coli) environment. We here demonstrate its utility for studying mammalian protein interaction…
View article: Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research
Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research Open
Bacterial two-hybrid (B2H) systems offer a versatile platform for detecting protein-protein interactions (PPIs) in vivo . Originally developed to study bacterial transcriptional regulators, these systems have evolved to support a wide rang…
View article: Exploration of Potential Broad-Spectrum Antiviral Targets in the Enterovirus Replication Element: Identification of Six Distinct 5′ Cloverleaves
Exploration of Potential Broad-Spectrum Antiviral Targets in the Enterovirus Replication Element: Identification of Six Distinct 5′ Cloverleaves Open
Enterovirus genomic replication initiates at a predicted RNA cloverleaf (5′CL) at the 5′ end of the RNA genome. The 5′CL contains one stem (SA) and three stem-loops (SLB, SLC, SLD). Here, we present an analysis of 5′CL conservation and div…
View article: Evidence of direct interaction between cisplatin and the caspase‐cleaved prostate apoptosis response‐4 tumor suppressor
Evidence of direct interaction between cisplatin and the caspase‐cleaved prostate apoptosis response‐4 tumor suppressor Open
Prostate apoptosis response‐4 (Par‐4) tumor suppressor protein has gained attention as a potential therapeutic target owing to its unique ability to selectively induce apoptosis in cancer cells, sensitize them to chemotherapy and radiother…
View article: Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis
Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis Open
Intrinsically disordered proteins play important roles in cell signaling, and dysregulation of these proteins is associated with several diseases. Prostate apoptosis response-4 (Par-4), an approximately 40 kilodalton proapoptotic tumor sup…
View article: Influence of Monovalent and Divalent Ions in the Conformational Change of Caspase-Cleaved Par-4 (cl-Par-4) Tumor Suppressor Protein
Influence of Monovalent and Divalent Ions in the Conformational Change of Caspase-Cleaved Par-4 (cl-Par-4) Tumor Suppressor Protein Open
Prostate apoptosis response-4 (Par-4) is a pro-apoptotic tumor suppressor protein. We have shown that this 38 kDa full-length Par-4 (Fl-Par-4) protein is predominantly intrinsically disordered in vitro. In vivo, Par-4 is cleaved by caspase…
View article: Structural Analysis of the cl-Par-4 Tumor Suppressor as a Function of Ionic Environment
Structural Analysis of the cl-Par-4 Tumor Suppressor as a Function of Ionic Environment Open
Prostate apoptosis response-4 (Par-4) is a proapoptotic tumor suppressor protein that has been linked to a large number of cancers. This 38 kilodalton (kDa) protein has been shown to be predominantly intrinsically disordered in vitro. In v…
View article: Methylation of the D2 dopamine receptor affects binding with the human regulatory proteins Par-4 and Calmodulin
Methylation of the D2 dopamine receptor affects binding with the human regulatory proteins Par-4 and Calmodulin Open
Protein arginine methylation is a post-translational modification that contributes to the regulation of many cellular processes, including DNA damage repair, transcriptional regulation and protein-protein interaction (Bedford and Richard 2…
View article: Structural Biology of the Enterovirus Replication-Linked 5′-Cloverleaf RNA and Associated Virus Proteins
Structural Biology of the Enterovirus Replication-Linked 5′-Cloverleaf RNA and Associated Virus Proteins Open
Although enteroviruses are associated with a wide variety of diseases and conditions, their mode of replication is well conserved. Their genome is carried as a single, positive-sense RNA strand. At the 5′ end of the strand is an approximat…
View article: Tetramer formation by the caspase‐activated fragment of the Par‐4 tumor suppressor
Tetramer formation by the caspase‐activated fragment of the Par‐4 tumor suppressor Open
The prostate apoptosis response‐4 (Par‐4) tumor suppressor can selectively kill cancer cells via apoptosis while leaving healthy cells unharmed. Full length Par‐4 has been shown to be predominantly intrinsically disordered in vitro under n…
View article: Conformational flexibility in the enterovirus RNA replication platform
Conformational flexibility in the enterovirus RNA replication platform Open
A presumed RNA cloverleaf (5′CL), located at the 5′-most end of the noncoding region of the enterovirus genome, is the primary established site for initiation of genomic replication. Stem–loop B (SLB) and stem–loop D (SLD), the two largest…
View article: pH-Induced Folding of the Caspase-Cleaved Par-4 Tumor Suppressor: Evidence of Structure Outside of the Coiled Coil Domain
pH-Induced Folding of the Caspase-Cleaved Par-4 Tumor Suppressor: Evidence of Structure Outside of the Coiled Coil Domain Open
Prostate apoptosis response-4 (Par-4) is a 38 kDa largely intrinsically disordered tumor suppressor protein that functions in cancer cell apoptosis. Par-4 down-regulation is often observed in cancer while up-regulation is characteristic of…
View article: Structure of RNA Stem Loop B from the Picornavirus Replication Platform
Structure of RNA Stem Loop B from the Picornavirus Replication Platform Open
The presumptive RNA cloverleaf at the start of the 5'-untranslated region of the picornavirus genome is an essential element in replication. Stem loop B (SLB) of the cloverleaf is a recognition site for the host polyC-binding protein, whic…
View article: CCDC 1443638: Experimental Crystal Structure Determination
CCDC 1443638: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …