Subhas Hajeri
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View article: Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation
Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation Open
This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed by “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those agents remain on regulat…
View article: Virus-induced gene silencing simultaneously exploits ‘attract and kill’ traits in plants and insects to manage huanglongbing
Virus-induced gene silencing simultaneously exploits ‘attract and kill’ traits in plants and insects to manage huanglongbing Open
The vector-borne disease huanglongbing (HLB) causes severe economic losses to citrus production worldwide with no available cure. Herein, we applied virus-induced gene silencing technology to engineer citrus that preferentially attracted a…
View article: Alternative Tissue Sampling for Improved Detection of Candidatus Liberibacter asiaticus
Alternative Tissue Sampling for Improved Detection of Candidatus Liberibacter asiaticus Open
Early detection and prompt response are key factors in the eradication of ‘huanglongbing’ (HLB) in California. Currently, qPCR testing of leaf tissue guides the removal of infected trees. However, because of the uneven distribution of ‘Can…
View article: Survey estimates of the incidence and diversity of Citrus tristeza virus in California
Survey estimates of the incidence and diversity of Citrus tristeza virus in California Open
Surveys were conducted to assess Citrus tristeza virus (CTV) in California. Orchard surveys in central California during 2009 to 2013 estimated CTV incidence from 0.05% to 2.9%. Similar surveys in Ventura, Riverside, San Bernardino, and Sa…
View article: Amino acid, sugar, phenolic, and terpenoid profiles are capable of distinguishing Citrus tristeza virus infection status in citrus cultivars: Grapefruit, lemon, mandarin, and sweet orange
Amino acid, sugar, phenolic, and terpenoid profiles are capable of distinguishing Citrus tristeza virus infection status in citrus cultivars: Grapefruit, lemon, mandarin, and sweet orange Open
Citrus tristeza virus (CTV) is the most severe viral disease for citrus production. Many strains of CTV have been characterized and their symptomology widely varies, ranging from asymptomatic or mild infections to severe symptomology that …
View article: Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Background Spiroplasma citri comprises a bacterial complex that cause diseases in citrus, horseradish, carrot, sesame, and also infects a wide array of ornamental and weed species. S. citri is transmitted in a persistent propagative manner…
View article: Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR
Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR Open
“ Candidatus Liberibacter asiaticus” ( C Las) and Spiroplasma citri are phloem-limited bacteria that infect citrus and are transmitted by insect vectors. S . citri causes citrus stubborn disease (CSD) and is vectored by the beet leafhopper…
View article: Additional file 1 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Additional file 1 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Additional file 1: Supplementary Table S1. BLASTn similarity search of Spiroplasma citri putative plasmid (contigs) against available Spiroplasma sequences deposited in NCBI database.
View article: Additional file 2 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Additional file 2 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Additional file 2: Supplementary Table S2. GenBank accession numbers for the 16S rRNA Spiroplasma sequences used for phylogenetic analyses in Fig. 1.
View article: Additional file 3 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Additional file 3 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Additional file 3: Supplementary Table S3. COG functional categories of Spiroplasma citri LB319
View article: Additional file 4 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Additional file 4 of Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Additional file 4: Supplementary Table S4. Summary of PHAge Search Tool Enhanced Release (PHASTER) analysis of Spiroplasma citri strains
View article: Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors Open
Background Spiroplasma citri comprises a complex of bacteria that cause diseases in citrus, horseradish, carrot, sesame, and also infect a wide array of ornamental and weed species. S. citri is transmitted in a persistent propagative manne…
View article: Multiplex detection of “<i>Candidatus</i>Liberibacter asiaticus” and<i>Spiroplasma citri</i>by qPCR and droplet digital PCR
Multiplex detection of “<i>Candidatus</i>Liberibacter asiaticus” and<i>Spiroplasma citri</i>by qPCR and droplet digital PCR Open
“ Candidatus Liberibacter asiaticus” ( C Las) and Spiroplasma citri are phloem-limited bacteria that infect citrus and are transmitted by insect vectors. S. citri causes citrus stubborn disease (CSD) and is vectored by the beet leafhopper …
View article: Genome Analysis of Spiroplasma Citri Strains From Different Host Plants and Its Leafhopper Vectors
Genome Analysis of Spiroplasma Citri Strains From Different Host Plants and Its Leafhopper Vectors Open
Background Spiroplasma citri comprises a complex of bacteria that cause diseases in citrus, horseradish, carrot, sesame, and also infect a wide array of ornamental and weed species. S. citri is transmitted in a persistent propagative manne…
View article: Spread of Citrus Tristeza Virus in Citrus Orchards in Central California
Spread of Citrus Tristeza Virus in Citrus Orchards in Central California Open
In California, citrus tristeza virus (CTV) is regulated by a State Interior Quarantine. In CTV abatement districts in central California, trees with CTV that react to MCA13 (MCA13-positive [MCA13+]), a strain-discriminating monoclonal anti…
View article: Reliable Sampling Tissue and Seasonality for Consistent Detection of ‘Candidatus Liberibacter asiaticus’ by qPCR
Reliable Sampling Tissue and Seasonality for Consistent Detection of ‘Candidatus Liberibacter asiaticus’ by qPCR Open
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View article: A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture-reverse transcriptase loop-mediated isothermal amplification assay
A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture-reverse transcriptase loop-mediated isothermal amplification assay Open
Severe strains of Citrus tristeza virus (CTV) cause quick decline and stem pitting resulting in significant economic losses in citrus production. A immunocapture reverse-transcriptase loop-mediated amplification (IC-RT-LAMP) assay was deve…
View article: Effects of <i>δ</i>‐aminolevulinic acid dehydratase silencing on the primary and secondary metabolisms of citrus
Effects of <i>δ</i>‐aminolevulinic acid dehydratase silencing on the primary and secondary metabolisms of citrus Open
δ ‐aminolevulinic acid dehydratase (ALAD) is an important enzyme in tetrapyrrole synthesis. ALAD combines two δ ‐aminolevulinic acid ( δ ‐ALA) molecules to form the pyrrole molecule, porphobilinogen, an important precursor for plant pigmen…
View article: Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus"
Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus" Open
Huanglongbing (HLB, citrus greening) is a devastating citrus disease affecting citrus production worldwide. It is associated with the bacterium "Candidatus Liberibacter asiaticus" (CLas) and is vectored by the Asian citrus psyllid (ACP). C…
View article: Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR
Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR Open
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without th…
View article: Identification and Characterization of<i>Citrus tristeza virus</i>Isolates Breaking Resistance in Trifoliate Orange in California
Identification and Characterization of<i>Citrus tristeza virus</i>Isolates Breaking Resistance in Trifoliate Orange in California Open
Most Citrus tristeza virus (CTV) isolates in California are biologically mild and symptomless in commercial cultivars on CTV tolerant rootstocks. However, to better define California CTV isolates showing divergent serological and genetic p…