Suckjoon Jun
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View article: Robust and resource-optimal dynamic pattern formation of Min proteins in vivo
Robust and resource-optimal dynamic pattern formation of Min proteins in vivo Open
The Min protein system prevents abnormal cell division in bacteria by forming oscillatory patterns between cell poles. However, predicting the protein concentrations at which oscillations start and whether cells can maintain them under phy…
View article: Dispensability of extrinsic DnaA regulators in <i>Escherichia coli</i> cell-cycle control
Dispensability of extrinsic DnaA regulators in <i>Escherichia coli</i> cell-cycle control Open
Investigating a long-standing conceptual question in bacterial physiology, we examine why DnaA, the bacterial master replication initiator protein, exists in both ATP and ADP forms, despite only the ATP form being essential for initiation.…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Author Response: Tools and methods for high-throughput single-cell imaging with the mother machine
Author Response: Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Reviewer #1 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine
Reviewer #1 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Reviewer #2 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine
Reviewer #2 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Author Response: Tools and methods for high-throughput single-cell imaging with the mother machine
Author Response: Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Bacterial Replication Initiation as Precision Control by Protein Counting
Bacterial Replication Initiation as Precision Control by Protein Counting Open
Balanced biosynthesis is the hallmark of bacterial cell physiology, where the concentrations of stable proteins remain steady. However, this poses a conceptual challenge to modeling the cell-cycle and cell-size controls in bacteria, as pre…
View article: Robust and resource-optimal dynamic pattern formation of Min proteins<i>in vivo</i>
Robust and resource-optimal dynamic pattern formation of Min proteins<i>in vivo</i> Open
The Min system in Escherichia coli plays a crucial role in cellular reproduction by preventing minicell formation through pole-to-pole oscillations. Despite extensive research, predicting the onset of Min protein concentrations for oscilla…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Reviewer #1 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine
Reviewer #1 (Public Review): Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Replication initiation in bacteria: precision control based on protein counting
Replication initiation in bacteria: precision control based on protein counting Open
Balanced biosynthesis is the hallmark of bacterial cell physiology, where the concentrations of stable proteins remain steady. However, this poses a conceptual challenge to modeling the cell-cycle and cell-size controls in bacteria, as pre…
View article: Tools and methods for high-throughput single-cell imaging with the mother machine
Tools and methods for high-throughput single-cell imaging with the mother machine Open
Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of mi…
View article: Robust control of replication initiation in the absence of DnaA-ATP ↔ DnaA-ADP regulatory elements in<i>Escherichia coli</i>
Robust control of replication initiation in the absence of DnaA-ATP ↔ DnaA-ADP regulatory elements in<i>Escherichia coli</i> Open
Investigating a long-standing conceptual question in bacterial physiology, we examine why DnaA, the bacterial master replication initiator protein, exists in both ATP and ADP forms, despite only the ATP form being essential for initiation.…
View article: Quantitative Examination of Five Stochastic Cell-Cycle and Cell-Size Control Models for Escherichia coli and Bacillus subtilis
Quantitative Examination of Five Stochastic Cell-Cycle and Cell-Size Control Models for Escherichia coli and Bacillus subtilis Open
We examine five quantitative models of the cell-cycle and cell-size control in Escherichia coli and Bacillus subtilis that have been proposed over the last decade to explain single-cell experimental data generated with high-throughput meth…
View article: Quantitative examination of five stochastic cell-cycle and cell-size control models for<i>Escherichia coli</i>and<i>Bacillus subtilis</i>
Quantitative examination of five stochastic cell-cycle and cell-size control models for<i>Escherichia coli</i>and<i>Bacillus subtilis</i> Open
We examine five quantitative models of the cell-cycle and cell-size control in Escherichia coli and Bacillus subtilis that have been proposed over the last decade to explain single-cell experimental data generated with high-throughput meth…
View article: Single-cell data and correlation analysis support the independent double adder model in both<i>Escherichia coli</i>and<i>Bacillus subtilis</i>
Single-cell data and correlation analysis support the independent double adder model in both<i>Escherichia coli</i>and<i>Bacillus subtilis</i> Open
The reference point for cell-size control in the cell cycle is a fundamental biological question. We previously reported that we were unable to reproduce the conclusions of Witz et al. ’s eLife paper (Witz, van Nimwegen, and Julou 2019) en…
View article: Comment on ‘Initiation of chromosome replication controls both division and replication cycles in<i>E. coli</i>through a double-adder mechanism’
Comment on ‘Initiation of chromosome replication controls both division and replication cycles in<i>E. coli</i>through a double-adder mechanism’ Open
Witz et al . recently performed single-cell mother machine experiments to track growth and the replication cycle in E. coli . They analyzed the correlation structure of selected parameters using both their data and published data, and conc…
View article: Control of Bacillus subtilis Replication Initiation during Physiological Transitions and Perturbations
Control of Bacillus subtilis Replication Initiation during Physiological Transitions and Perturbations Open
High-throughput, quantitative approaches have enabled the discovery of fundamental principles describing bacterial physiology. These principles provide a foundation for predicting the behavior of biological systems, a widely held aspiratio…
View article: Mother machine image analysis with MM3
Mother machine image analysis with MM3 Open
The mother machine is a microfluidic device for high-throughput time-lapse imaging of microbes. Here, we present MM3, a complete and modular image analysis pipeline. MM3 turns raw mother machine images, both phase contrast and fluorescence…
View article: Gram-positive and Gram-negative Bacteria Share Common Principles to Coordinate Growth and the Cell Cycle at the Single-cell Level
Gram-positive and Gram-negative Bacteria Share Common Principles to Coordinate Growth and the Cell Cycle at the Single-cell Level Open
Bacillus subtilis and Escherichia coli are evolutionarily divergent model organisms that have elucidated fundamental differences between Gram-positive and Gram-negative bacteria, respectively. Despite their differences in cell cycle contro…
View article: Mechanistic origin of cell-size control and homeostasis in bacteria
Mechanistic origin of cell-size control and homeostasis in bacteria Open
Evolutionarily divergent bacteria share a common phenomenological strategy for cell-size homeostasis under steady-state conditions. In the presence of inherent physiological stochasticity, cells following this “adder” principle gradually r…