Sumio Ohtsuki
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View article: Inhibitory Effect of Insulin-Degrading Enzyme-Selective Inhibitor, Ii1, on the Elimination of Amyloid-<i>β</i>(1-40) from Rat Brain
Inhibitory Effect of Insulin-Degrading Enzyme-Selective Inhibitor, Ii1, on the Elimination of Amyloid-<i>β</i>(1-40) from Rat Brain Open
Amyloid-β peptide (Aβ) is eliminated from the brain across the blood-brain barrier (BBB) by an insulin-sensitive process. To investigate the involvement of insulin-degrading enzyme (IDE) in this process, the present study was implemented t…
View article: Fibroblast‐specific palladin drives kidney fibrosis via <scp>MRTF</scp> – <scp>SRF</scp> signaling
Fibroblast‐specific palladin drives kidney fibrosis via <span>MRTF</span> – <span>SRF</span> signaling Open
Fibrosis is a common end‐stage pathway of progressive chronic kidney diseases. Previously we demonstrated that myocardin‐related transcription factor (MRTF)–serum response factor (SRF) signaling drives the expression of fibrosis‐related mo…
View article: The hook-like adaptor and cargo-binding (HAC) domain in the kinesin-2 tail enables adaptor assembly and cargo recognition
The hook-like adaptor and cargo-binding (HAC) domain in the kinesin-2 tail enables adaptor assembly and cargo recognition Open
Intracellular transport relies on motor proteins such as kinesins to deliver cargo along microtubules, yet how they recognize cargo remains unclear. Here, we present high-resolution cryo–electron microscopy structures of the heterotrimeric…
View article: Ataxin-2 as a candidate blood biomarker for estimating disease status in cases of suspected glioblastoma recurrence
Ataxin-2 as a candidate blood biomarker for estimating disease status in cases of suspected glioblastoma recurrence Open
Differentiating pseudoprogression (PsP) from recurrence in cases of glioblastoma (GBM) after chemoradiotherapy is challenging, with neuroimaging as the only non-invasive method. In this study, we aimed to identify a blood biomarker for pre…
View article: Comparative Proteomic Analysis of Endocytic Cell-Surface Proteins in Vascular and Lymphatic Endothelial Cells
Comparative Proteomic Analysis of Endocytic Cell-Surface Proteins in Vascular and Lymphatic Endothelial Cells Open
Vascular endothelial cells (VECs) and lymphatic endothelial cells (LECs) regulate the homeostasis of fluids, nutrients, and immune cells in the blood and lymphatic systems, respectively. These specialized functions depend on distinct cell-…
View article: Effects of SLC6A8 mutation-induced creatine deficiency on cellular function in fibroblasts
Effects of SLC6A8 mutation-induced creatine deficiency on cellular function in fibroblasts Open
Creatine transporter deficiency (CTD) caused by mutations in SLC6A8 encoding the creatine transporter (CRT), leads to cerebral creatine deficiency syndromes; however, the cellular impact of CRT loss remains unclear. In this study, we inves…
View article: Proteome profile differences among human, monkey, and mouse brain microvessels and cultured brain microvascular endothelial cells
Proteome profile differences among human, monkey, and mouse brain microvessels and cultured brain microvascular endothelial cells Open
Deep proteomic analysis revealed species-specific differences in transport-related proteins in the BBB. Furthermore, in vitro and in vivo differences were observed in the transporter and claudin protein expression. This study provides a BB…
View article: Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growth
Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growth Open
View article: The Hitchdock Domain in Kinesin-2 Tail Enables Adaptor Assembly and Cargo Binding
The Hitchdock Domain in Kinesin-2 Tail Enables Adaptor Assembly and Cargo Binding Open
Intracellular transport relies on motor proteins like kinesins to deliver essential cargoes along microtubules, yet the mechanisms of cargo recognition remain unclear. Here, we present high-resolution cryo-electron microscopy structures of…
View article: HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis
HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis Open
View article: PROTOTYPIC ANTIDEPRESSANTS ACT AS A G-PROTEIN BIASED LPAR1 AGONIST AND CONTRIBUTE TO ANTIDEPRESSANT EFFECTS
PROTOTYPIC ANTIDEPRESSANTS ACT AS A G-PROTEIN BIASED LPAR1 AGONIST AND CONTRIBUTE TO ANTIDEPRESSANT EFFECTS Open
Background Prototypic antidepressants, such as tricyclic/tetracyclic antidepressants (TCAs), have multiple pharmacological properties and have been considered to be more effective than newer antidepressants, such as selective serotonin reu…
View article: Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis
Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis Open
We successfully developed a method for isolation of brain microvessels from a small amount of frozen human brain tissue, facilitating detailed study of BBB proteome in aging or pathological conditions. This technique provides valuable insi…
View article: Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice
Fibrillin-1 G234D mutation in the hybrid1 domain causes tight skin associated with dysregulated elastogenesis and increased collagen cross-linking in mice Open
View article: Increased CSN5 expression enhances the sensitivity to lenalidomide in multiple myeloma cells
Increased CSN5 expression enhances the sensitivity to lenalidomide in multiple myeloma cells Open
View article: Identification of <scp>TPI1</scp> As a potential therapeutic target in pancreatic cancer with dependency of <scp><i>TP53</i></scp> mutation using multi‐omics analysis
Identification of <span>TPI1</span> As a potential therapeutic target in pancreatic cancer with dependency of <span><i>TP53</i></span> mutation using multi‐omics analysis Open
Mutations of KRAS , CDKN2A , TP53 , and SMAD4 are the four major driver genes for pancreatic ductal adenocarcinoma (PDAC), of which mutations of KRAS and TP53 are the most frequently recognized. However, molecular‐targeted therapies for mu…
View article: Isolation Method of Brain Microvessels from Minimal Frozen Human Brain Tissue for Blood-Brain Barrier Protein Expression Analysis
Isolation Method of Brain Microvessels from Minimal Frozen Human Brain Tissue for Blood-Brain Barrier Protein Expression Analysis Open
Background Protein expression analysis of isolated brain microvessels provides valuable insights into the function of the blood-brain barrier (BBB). However, the isolation of brain microvessels from human brain tissue, particularly in smal…
View article: Bakuchiol targets mitochondrial proteins, prohibitins and voltage-dependent anion channels: New insights into developing antiviral agents
Bakuchiol targets mitochondrial proteins, prohibitins and voltage-dependent anion channels: New insights into developing antiviral agents Open
We previously reported that bakuchiol, a phenolic isoprenoid anticancer compound, and its analogs exert anti-influenza activity. However, the proteins targeted by bakuchiol remain unclear. Here, we investigated the chemical structures resp…
View article: Advancement of Water Droplet-in-Oil Digestion Method for High-Throughput Single-Cell Proteomics
Advancement of Water Droplet-in-Oil Digestion Method for High-Throughput Single-Cell Proteomics Open
Protein expression varies between cells, and observing this heterogeneity helps to understand biological phenomena. We have developed a water droplet-in-oil digestion (WinO) method for single-cell proteomics. The WinO method reduces adsorp…
View article: Proteomic alterations in the brain and blood–brain barrier during brain Aβ accumulation in an APP knock-in mouse model of Alzheimer’s disease
Proteomic alterations in the brain and blood–brain barrier during brain Aβ accumulation in an APP knock-in mouse model of Alzheimer’s disease Open
Background Blood–brain barrier (BBB) dysfunction is supposed to be an early event in the development of Alzheimer’s disease (AD). This study aimed to investigate the relationship between BBB alterations and AD progression in terms of amylo…
View article: Development and Functional Evaluation of MDR1-expressing Microvascular Endothelial-like Cells Derived from Human iPS Cells as an In vitro Blood-brain Barrier Model
Development and Functional Evaluation of MDR1-expressing Microvascular Endothelial-like Cells Derived from Human iPS Cells as an In vitro Blood-brain Barrier Model Open
In order to establish an in vitro model of the human blood-brain barrier (BBB), MDR1-overexpressing human induced pluripotent stem cells (hiPSCs) were generated, and they were differentiated to MDR1-expressing brain microvascular endotheli…
View article: G protein-biased LPAR1 agonism of prototypic antidepressants: Implication in the identification of novel therapeutic target for depression
G protein-biased LPAR1 agonism of prototypic antidepressants: Implication in the identification of novel therapeutic target for depression Open
Prototypic antidepressants, such as tricyclic/tetracyclic antidepressants (TCAs), have multiple pharmacological properties and have been considered to be more effective than newer antidepressants, such as selective serotonin reuptake inhib…
View article: <scp>SETD1A</scp> function in leukemia is mediated through interaction with mitotic regulators <scp>BuGZ</scp>/<scp>BUB3</scp>
<span>SETD1A</span> function in leukemia is mediated through interaction with mitotic regulators <span>BuGZ</span>/<span>BUB3</span> Open
The H3K4 methyltransferase SETD1A plays a crucial role in leukemia cell survival through its noncatalytic FLOS domain‐mediated recruitment of cyclin K and regulation of DNA damage response genes. In this study, we identify a functional nuc…
View article: Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries
Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries Open
View article: Supplementary Figure S4 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S4 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S4 shows that KDM5 inhibition of KDM5A knockdown downregulates MYC target genes in MM.
View article: Supplementary Figure S3 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S3 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S3 shows that JQKD82 have good activities against KDM5 in MM cells and in animal models.
View article: Supplementary Figure S3 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S3 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S3 shows that JQKD82 have good activities against KDM5 in MM cells and in animal models.
View article: Supplementary Table S2 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Table S2 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Table S2 shows the ChIP-seq data with the peaks significantly overlap with KDM5A ChIP-seq peaks MM1S
View article: Supplementary Figure S1 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S1 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S1 shows that higher KDM5A expression is associated with poor prognosis in MM.
View article: Supplementary Figure S6 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S6 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S6 shows that genome-wide H3K4me3 and RNAPII changes after treatment with JQKD82 or knockdown of KDM5A in MM.1S cells
View article: Supplementary Figure S6 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma
Supplementary Figure S6 from Lysine Demethylase 5A Is Required for MYC-Driven Transcription in Multiple Myeloma Open
Supplementary Figure S6 shows that genome-wide H3K4me3 and RNAPII changes after treatment with JQKD82 or knockdown of KDM5A in MM.1S cells